Restriction map for
pFN21AE1850
(Linear) (Six-base) MAPSORT of: KIEE1850.seq Check: 457 from: 1 to: 1107
KIEE1850.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 275 1107
Size: 275 832
Acc65I G'GTAC_C
Cuts at: 0 233 1107
Size: 233 874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 686 1107
Size: 491 195 421
Fragments arranged by size:
491 421 195
AflIII A'CryG_T
Cuts at: 0 678 1107
Size: 678 429
AlwNI CAG_nnn'CTG
Cuts at: 0 1021 1107
Size: 1021 86
BanI G'GyrC_C
Cuts at: 0 233 372 1107
Size: 233 139 735
Fragments arranged by size:
735 233 139
BanII G_rGCy'C
Cuts at: 0 25 1058 1107
Size: 25 1033 49
Fragments arranged by size:
1033 49 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1107
Size: 80 1027
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 1107
Size: 27 1080
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 1107
Size: 15 1092
BseYI C'CCAG_C
Cuts at: 0 18 51 1014 1107
Size: 18 33 963 93
Fragments arranged by size:
963 93 33 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 1107
Size: 1056 51
BsiHKAI G_wGCw'C
Cuts at: 0 1058 1107
Size: 1058 49
BsmI GAATG_Cn'
Cuts at: 0 875 923 1107
Size: 875 48 184
Fragments arranged by size:
875 184 48
Bsp1286I G_dGCh'C
Cuts at: 0 25 1058 1107
Size: 25 1033 49
Fragments arranged by size:
1033 49 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 802 1107
Size: 802 305
BsrGI T'GTAC_A
Cuts at: 0 251 1107
Size: 251 856
BstAPI GCAn_nnn'nTGC
Cuts at: 0 985 1107
Size: 985 122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 127 1107
Size: 127 980
Bsu36I CC'TnA_GG
Cuts at: 0 367 1107
Size: 367 740
DraI TTT'AAA
Cuts at: 0 1037 1107
Size: 1037 70
EaeI y'GGCC_r
Cuts at: 0 9 1107
Size: 9 1098
EarI CTCTTCn'nnn_
Cuts at: 0 353 611 623 1046 1107
Size: 353 258 12 423 61
Fragments arranged by size:
423 353 258 61 12
EcoICRI GAG'CTC
Cuts at: 0 1056 1107
Size: 1056 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 491 686 1107
Size: 80 411 195 421
Fragments arranged by size:
421 411 195 80
EcoO109I rG'GnC_Cy
Cuts at: 0 55 1107
Size: 55 1052
HincII GTy'rAC
Cuts at: 0 276 479 1107
Size: 276 203 628
Fragments arranged by size:
628 276 203
HpaI GTT'AAC
Cuts at: 0 479 1107
Size: 479 628
KpnI G_GTAC'C
Cuts at: 0 237 1107
Size: 237 870
MslI CAynn'nnrTG
Cuts at: 0 953 1107
Size: 953 154
MspA1I CmG'CkG
Cuts at: 0 281 1084 1107
Size: 281 803 23
Fragments arranged by size:
803 281 23
NdeI CA'TA_TG
Cuts at: 0 175 1107
Size: 175 932
NspI r_CATG'y
Cuts at: 0 682 1107
Size: 682 425
PciI A'CATG_T
Cuts at: 0 678 1107
Size: 678 429
PfoI T'CCnGG_A
Cuts at: 0 578 1004 1107
Size: 578 426 103
Fragments arranged by size:
578 426 103
PstI C_TGCA'G
Cuts at: 0 639 1107
Size: 639 468
PvuII CAG'CTG
Cuts at: 0 1084 1107
Size: 1084 23
SacI G_AGCT'C
Cuts at: 0 1058 1107
Size: 1058 49
SalI G'TCGA_C
Cuts at: 0 274 1107
Size: 274 833
SapI GCTCTTCn'nnn_
Cuts at: 0 1046 1107
Size: 1046 61
ScaI AGT'ACT
Cuts at: 0 258 1107
Size: 258 849
SfcI C'TryA_G
Cuts at: 0 136 189 635 1107
Size: 136 53 446 472
Fragments arranged by size:
472 446 136 53
StyI C'CwwG_G
Cuts at: 0 238 782 1107
Size: 238 544 325
Fragments arranged by size:
544 325 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1028 1107
Size: 1028 79
TatI w'GTAC_w
Cuts at: 0 251 256 596 1107
Size: 251 5 340 511
Fragments arranged by size:
511 340 251 5
XbaI T'CTAG_A
Cuts at: 0 690 1107
Size: 690 417
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI BanI BanII BpmI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrGI BstAPI BstXI Bsu36I DraI EaeI EarI EcoICRI
Eco57MI EcoO109I HincII HpaI KpnI MslI MspA1I NdeI
NspI PciI PfoI PstI PvuII SacI SalI SapI
ScaI SfcI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII KasI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI