Restriction map for
pFN21AE1849
(Linear) (Six-base) MAPSORT of: KIEE1849.seq Check: 4835 from: 1 to: 1095
KIEE1849.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 829 977 1095
Size: 587 242 148 118
Fragments arranged by size:
587 242 148 118
AlwNI CAG_nnn'CTG
Cuts at: 0 533 837 948 1095
Size: 533 304 111 147
Fragments arranged by size:
533 304 147 111
ApaI G_GGCC'C
Cuts at: 0 560 1095
Size: 560 535
ApoI r'AATT_y
Cuts at: 0 675 1095
Size: 675 420
AvaI C'yCGr_G
Cuts at: 0 1077 1095
Size: 1077 18
BanII G_rGCy'C
Cuts at: 0 560 1095
Size: 560 535
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 223 293 739 925 1095
Size: 128 95 70 446 186 170
Fragments arranged by size:
446 186 170 128 95 70
BciVI GTATCCnnnnn_n'
Cuts at: 0 107 1095
Size: 107 988
BfrBI ATG'CAT
Cuts at: 0 993 1095
Size: 993 102
BglI GCCn_nnn'nGGC
Cuts at: 0 428 1095
Size: 428 667
BlpI GC'TnA_GC
Cuts at: 0 542 608 1095
Size: 542 66 487
Fragments arranged by size:
542 487 66
Bme1580I G_kGCm'C
Cuts at: 0 560 1095
Size: 560 535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 776 1095
Size: 340 436 319
Fragments arranged by size:
436 340 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 1095
Size: 339 756
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1095
Size: 787 308
BsaAI yAC'GTr
Cuts at: 0 279 1095
Size: 279 816
BsaHI Gr'CG_yC
Cuts at: 0 506 1095
Size: 506 589
BsaWI w'CCGG_w
Cuts at: 0 842 930 1095
Size: 842 88 165
Fragments arranged by size:
842 165 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 195 225 337 367 898 928 1095
Size: 195 30 112 30 531 30 167
Fragments arranged by size:
531 195 167 112 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 919 1095
Size: 919 176
BseYI C'CCAG_C
Cuts at: 0 431 455 1095
Size: 431 24 640
Fragments arranged by size:
640 431 24
Bsp1286I G_dGCh'C
Cuts at: 0 560 1095
Size: 560 535
BspEI T'CCGG_A
Cuts at: 0 842 1095
Size: 842 253
BsrDI GCAATG_nn'
Cuts at: 0 122 1095
Size: 122 973
BssSI C'ACGA_G
Cuts at: 0 638 1095
Size: 638 457
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 1095
Size: 837 258
BstEII G'GTnAC_C
Cuts at: 0 85 1095
Size: 85 1010
BstXI CCAn_nnnn'nTGG
Cuts at: 0 431 783 1095
Size: 431 352 312
Fragments arranged by size:
431 352 312
Bsu36I CC'TnA_GG
Cuts at: 0 295 1095
Size: 295 800
BtgI C'CryG_G
Cuts at: 0 109 368 1019 1041 1095
Size: 109 259 651 22 54
Fragments arranged by size:
651 259 109 54 22
BtsI GCAGTG_nn'
Cuts at: 0 477 1095
Size: 477 618
DraIII CAC_nnn'GTG
Cuts at: 0 858 1095
Size: 858 237
DrdI GACnn_nn'nnGTC
Cuts at: 0 511 1095
Size: 511 584
EaeI y'GGCC_r
Cuts at: 0 1060 1095
Size: 1060 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 587 829 977 1095
Size: 339 248 242 148 118
Fragments arranged by size:
339 248 242 148 118
EcoO109I rG'GnC_Cy
Cuts at: 0 530 556 557 571 825 1095
Size: 530 26 1 14 254 270
Fragments arranged by size:
530 270 254 26 14 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 872 904 1095
Size: 742 32 98 32 191
Fragments arranged by size:
742 191 98 32 32
HindIII A'AGCT_T
Cuts at: 0 362 1095
Size: 362 733
MfeI C'AATT_G
Cuts at: 0 144 1095
Size: 144 951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 1095
Size: 309 786
MslI CAynn'nnrTG
Cuts at: 0 95 1001 1095
Size: 95 906 94
Fragments arranged by size:
906 95 94
MspA1I CmG'CkG
Cuts at: 0 541 1095
Size: 541 554
NcoI C'CATG_G
Cuts at: 0 109 1041 1095
Size: 109 932 54
Fragments arranged by size:
932 109 54
NsiI A_TGCA'T
Cuts at: 0 995 1095
Size: 995 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 533 1095
Size: 533 562
PfoI T'CCnGG_A
Cuts at: 0 199 1095
Size: 199 896
PmlI CAC'GTG
Cuts at: 0 279 1095
Size: 279 816
PpuMI rG'GwC_Cy
Cuts at: 0 571 1095
Size: 571 524
PspOMI G'GGCC_C
Cuts at: 0 556 1095
Size: 556 539
PstI C_TGCA'G
Cuts at: 0 833 1095
Size: 833 262
SexAI A'CCwGG_T
Cuts at: 0 854 1095
Size: 854 241
SfcI C'TryA_G
Cuts at: 0 829 1095
Size: 829 266
StyI C'CwwG_G
Cuts at: 0 109 1041 1095
Size: 109 932 54
Fragments arranged by size:
932 109 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 1095
Size: 57 1038
TatI w'GTAC_w
Cuts at: 0 389 1095
Size: 389 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 898 930 1095
Size: 898 32 165
Fragments arranged by size:
898 165 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 871 1095
Size: 871 224
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BbsI BciVI
BfrBI BglI BlpI Bme1580I BplI BpmI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BsrDI
BssSI BstAPI BstEII BstXI Bsu36I BtgI BtsI DraIII
DrdI EaeI Eco57MI EcoO109I Hin4I HindIII MfeI MmeI
MslI MspA1I NcoI NsiI PflMI PfoI PmlI PpuMI
PspOMI PstI SexAI SfcI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstBI BstYI BstZ17I BtgZI
ClaI CspCI DraI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI