Restriction map for
pFN21AE1848
(Linear) (Six-base) MAPSORT of: KIEE1848.seq Check: 7028 from: 1 to: 1095
KIEE1848.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 649 781 820 1093 1095
Size: 649 132 39 273 2
Fragments arranged by size:
649 273 132 39 2
AccI GT'mk_AC
Cuts at: 0 1079 1095
Size: 1079 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 748 1095
Size: 205 543 347
Fragments arranged by size:
543 347 205
AfeI AGC'GCT
Cuts at: 0 301 442 1095
Size: 301 141 653
Fragments arranged by size:
653 301 141
AflII C'TTAA_G
Cuts at: 0 514 1095
Size: 514 581
AleI CACnn'nnGTG
Cuts at: 0 273 1095
Size: 273 822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 586 1095
Size: 291 295 509
Fragments arranged by size:
509 295 291
ApaLI G'TGCA_C
Cuts at: 0 266 868 1059 1095
Size: 266 602 191 36
Fragments arranged by size:
602 266 191 36
ApoI r'AATT_y
Cuts at: 0 541 1095
Size: 541 554
AvaI C'yCGr_G
Cuts at: 0 225 271 822 915 1095
Size: 225 46 551 93 180
Fragments arranged by size:
551 225 180 93 46
BanI G'GyrC_C
Cuts at: 0 459 699 793 919 935 1095
Size: 459 240 94 126 16 160
Fragments arranged by size:
459 240 160 126 94 16
BanII G_rGCy'C
Cuts at: 0 113 579 1095
Size: 113 466 516
Fragments arranged by size:
516 466 113
BbeI G_GCGC'C
Cuts at: 0 703 1095
Size: 703 392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 513 547 775 809 1095
Size: 513 34 228 34 286
Fragments arranged by size:
513 286 228 34 34
BclI T'GATC_A
Cuts at: 0 617 1095
Size: 617 478
BglI GCCn_nnn'nGGC
Cuts at: 0 157 787 826 902 1095
Size: 157 630 39 76 193
Fragments arranged by size:
630 193 157 76 39
Bme1580I G_kGCm'C
Cuts at: 0 270 872 922 1063 1095
Size: 270 602 50 141 32
Fragments arranged by size:
602 270 141 50 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 211 337 490 842 1095
Size: 157 54 126 153 352 253
Fragments arranged by size:
352 253 157 153 126 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 736 1095
Size: 328 408 359
Fragments arranged by size:
408 359 328
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 1095
Size: 628 467
BsaHI Gr'CG_yC
Cuts at: 0 700 1095
Size: 700 395
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 375 1087 1095
Size: 375 712 8
Fragments arranged by size:
712 375 8
BseYI C'CCAG_C
Cuts at: 0 625 1095
Size: 625 470
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 441 1071 1095
Size: 294 147 630 24
Fragments arranged by size:
630 294 147 24
BsiEI CG_ry'CG
Cuts at: 0 83 152 180 1095
Size: 83 69 28 915
Fragments arranged by size:
915 83 69 28
BsiHKAI G_wGCw'C
Cuts at: 0 270 872 1063 1095
Size: 270 602 191 32
Fragments arranged by size:
602 270 191 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 232 736 1095
Size: 232 504 359
Fragments arranged by size:
504 359 232
Bsp1286I G_dGCh'C
Cuts at: 0 113 270 579 872 922 1063 1095
Size: 113 157 309 293 50 141 32
Fragments arranged by size:
309 293 157 141 113 50 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 457 649 691 781 820 1093 1095
Size: 457 192 42 90 39 273 2
Fragments arranged by size:
457 273 192 90 42 39 2
BsrBI CCG'CTC
Cuts at: 0 195 1095
Size: 195 900
BsrFI r'CCGG_y
Cuts at: 0 179 781 1095
Size: 179 602 314
Fragments arranged by size:
602 314 179
BssHII G'CGCG_C
Cuts at: 0 22 1095
Size: 22 1073
BssSI C'ACGA_G
Cuts at: 0 601 1095
Size: 601 494
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1058 1095
Size: 1058 37
BstYI r'GATC_y
Cuts at: 0 567 1095
Size: 567 528
BtgI C'CryG_G
Cuts at: 0 35 651 808 975 1095
Size: 35 616 157 167 120
Fragments arranged by size:
616 167 157 120 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 467 1095
Size: 467 628
BtsI GCAGTG_nn'
Cuts at: 0 496 1063 1095
Size: 496 567 32
Fragments arranged by size:
567 496 32
EaeI y'GGCC_r
Cuts at: 0 32 80 149 410 903 927 972 978
Size: 32 48 69 261 493 24 45 6
Cuts at: 978 1095
Size: 117
Fragments arranged by size:
493 261 117 69 48 45 32 24
6
EagI C'GGCC_G
Cuts at: 0 80 149 1095
Size: 80 69 946
Fragments arranged by size:
946 80 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 205 211 337 490 748 842 1095
Size: 157 48 6 126 153 258 94 253
Fragments arranged by size:
258 253 157 153 126 94 48 6
EcoO109I rG'GnC_Cy
Cuts at: 0 11 222 1010 1095
Size: 11 211 788 85
Fragments arranged by size:
788 211 85 11
EcoRI G'AATT_C
Cuts at: 0 541 1095
Size: 541 554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 1095
Size: 369 726
HaeII r_GCGC'y
Cuts at: 0 303 444 703 1095
Size: 303 141 259 392
Fragments arranged by size:
392 303 259 141
KasI G'GCGC_C
Cuts at: 0 699 1095
Size: 699 396
MscI TGG'CCA
Cuts at: 0 412 1095
Size: 412 683
MslI CAynn'nnrTG
Cuts at: 0 273 737 875 1095
Size: 273 464 138 220
Fragments arranged by size:
464 273 220 138
MspA1I CmG'CkG
Cuts at: 0 18 37 129 204 846 925 977 1095
Size: 18 19 92 75 642 79 52 118
Fragments arranged by size:
642 118 92 79 75 52 19 18
NaeI GCC'GGC
Cuts at: 0 783 1095
Size: 783 312
NarI GG'CG_CC
Cuts at: 0 700 1095
Size: 700 395
NcoI C'CATG_G
Cuts at: 0 651 1095
Size: 651 444
NgoMIV G'CCGG_C
Cuts at: 0 781 1095
Size: 781 314
NotI GC'GGCC_GC
Cuts at: 0 149 1095
Size: 149 946
NspI r_CATG'y
Cuts at: 0 213 1095
Size: 213 882
PflMI CCAn_nnn'nTGG
Cuts at: 0 714 1095
Size: 714 381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 1095
Size: 265 32 798
Fragments arranged by size:
798 265 32
PpuMI rG'GwC_Cy
Cuts at: 0 11 222 1095
Size: 11 211 873
Fragments arranged by size:
873 211 11
PspXI vC'TCGA_Gb
Cuts at: 0 271 1095
Size: 271 824
SacII CC_GC'GG
Cuts at: 0 38 978 1095
Size: 38 940 117
Fragments arranged by size:
940 117 38
SanDI GG'GwC_CC
Cuts at: 0 11 222 1095
Size: 11 211 873
Fragments arranged by size:
873 211 11
SfoI GGC'GCC
Cuts at: 0 701 1095
Size: 701 394
SmaI CCC'GGG
Cuts at: 0 227 917 1095
Size: 227 690 178
Fragments arranged by size:
690 227 178
SmlI C'TyrA_G
Cuts at: 0 271 343 514 751 1095
Size: 271 72 171 237 344
Fragments arranged by size:
344 271 237 171 72
StuI AGG'CCT
Cuts at: 0 172 484 1095
Size: 172 312 611
Fragments arranged by size:
611 312 172
StyI C'CwwG_G
Cuts at: 0 413 651 1095
Size: 413 238 444
Fragments arranged by size:
444 413 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1095
Size: 595 500
TatI w'GTAC_w
Cuts at: 0 518 1095
Size: 518 577
XhoI C'TCGA_G
Cuts at: 0 271 1095
Size: 271 824
XmaI C'CCGG_G
Cuts at: 0 225 915 1095
Size: 225 690 180
Fragments arranged by size:
690 225 180
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AleI AlfI ApaLI
ApoI AvaI BanI BanII BbeI BcgI BclI BglI
Bme1580I BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstYI BtgI BtgZI BtsI EaeI EagI
Eco57MI EcoO109I EcoRI FalI HaeII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI
PpiI PpuMI PspXI SacII SanDI SfoI SmaI SmlI
StuI StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AhdI AloI AlwNI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XmnI ZraI