Restriction map for
pFN21AE1847
(Linear) (Six-base) MAPSORT of: KIEE1847.seq Check: 7425 from: 1 to: 1095
KIEE1847.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AccI GT'mk_AC
Cuts at: 0 656 1095
Size: 656 439
AleI CACnn'nnGTG
Cuts at: 0 994 1095
Size: 994 101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 972 1095
Size: 972 123
BanI G'GyrC_C
Cuts at: 0 217 310 346 1095
Size: 217 93 36 749
Fragments arranged by size:
749 217 93 36
BanII G_rGCy'C
Cuts at: 0 81 1095
Size: 81 1014
BbeI G_GCGC'C
Cuts at: 0 221 314 1095
Size: 221 93 781
Fragments arranged by size:
781 221 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 710 1095
Size: 710 385
BciVI GTATCCnnnnn_n'
Cuts at: 0 778 1095
Size: 778 317
BclI T'GATC_A
Cuts at: 0 278 1095
Size: 278 817
BlpI GC'TnA_GC
Cuts at: 0 337 471 1095
Size: 337 134 624
Fragments arranged by size:
624 337 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 501 907 1095
Size: 501 406 188
Fragments arranged by size:
501 406 188
BsaAI yAC'GTr
Cuts at: 0 1008 1095
Size: 1008 87
BsaHI Gr'CG_yC
Cuts at: 0 218 311 1095
Size: 218 93 784
Fragments arranged by size:
784 218 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 955 1095
Size: 955 140
BseYI C'CCAG_C
Cuts at: 0 132 225 1095
Size: 132 93 870
Fragments arranged by size:
870 132 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 968 1095
Size: 440 528 127
Fragments arranged by size:
528 440 127
BsmI GAATG_Cn'
Cuts at: 0 230 396 931 1095
Size: 230 166 535 164
Fragments arranged by size:
535 230 166 164
Bsp1286I G_dGCh'C
Cuts at: 0 81 1095
Size: 81 1014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 1095
Size: 203 892
BsrDI GCAATG_nn'
Cuts at: 0 104 605 1095
Size: 104 501 490
Fragments arranged by size:
501 490 104
BssHII G'CGCG_C
Cuts at: 0 38 1095
Size: 38 1057
BssSI C'ACGA_G
Cuts at: 0 58 259 1095
Size: 58 201 836
Fragments arranged by size:
836 201 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 926 1095
Size: 926 169
BtgI C'CryG_G
Cuts at: 0 569 669 1095
Size: 569 100 426
Fragments arranged by size:
569 426 100
BtsI GCAGTG_nn'
Cuts at: 0 423 1095
Size: 423 672
EaeI y'GGCC_r
Cuts at: 0 735 975 1095
Size: 735 240 120
Fragments arranged by size:
735 240 120
EcoRV GAT'ATC
Cuts at: 0 776 1095
Size: 776 319
FspI TGC'GCA
Cuts at: 0 802 1095
Size: 802 293
HaeII r_GCGC'y
Cuts at: 0 16 221 314 1095
Size: 16 205 93 781
Fragments arranged by size:
781 205 93 16
KasI G'GCGC_C
Cuts at: 0 217 310 1095
Size: 217 93 785
Fragments arranged by size:
785 217 93
MscI TGG'CCA
Cuts at: 0 737 1095
Size: 737 358
MslI CAynn'nnrTG
Cuts at: 0 614 842 994 1095
Size: 614 228 152 101
Fragments arranged by size:
614 228 152 101
MspA1I CmG'CkG
Cuts at: 0 153 344 665 1095
Size: 153 191 321 430
Fragments arranged by size:
430 321 191 153
NarI GG'CG_CC
Cuts at: 0 218 311 1095
Size: 218 93 784
Fragments arranged by size:
784 218 93
NspI r_CATG'y
Cuts at: 0 528 704 1095
Size: 528 176 391
Fragments arranged by size:
528 391 176
PasI CC'CwG_GG
Cuts at: 0 907 1095
Size: 907 188
PmlI CAC'GTG
Cuts at: 0 1008 1095
Size: 1008 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 1012 1044 1095
Size: 454 32 526 32 51
Fragments arranged by size:
526 454 51 32 32
PshAI GACnn'nnGTC
Cuts at: 0 1078 1095
Size: 1078 17
PstI C_TGCA'G
Cuts at: 0 387 1095
Size: 387 708
ScaI AGT'ACT
Cuts at: 0 590 1095
Size: 590 505
SexAI A'CCwGG_T
Cuts at: 0 406 1095
Size: 406 689
SfcI C'TryA_G
Cuts at: 0 383 1095
Size: 383 712
SfoI GGC'GCC
Cuts at: 0 219 312 1095
Size: 219 93 783
Fragments arranged by size:
783 219 93
StuI AGG'CCT
Cuts at: 0 389 1095
Size: 389 706
StyI C'CwwG_G
Cuts at: 0 81 1095
Size: 81 1014
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1006 1095
Size: 1006 89
TatI w'GTAC_w
Cuts at: 0 588 1095
Size: 588 507
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 661 693 1095
Size: 661 32 402
Fragments arranged by size:
661 402 32
Tth111I GACn'n_nGTC
Cuts at: 0 408 1095
Size: 408 687
Enzymes that do cut:
AccI AleI AlfI BanI BanII BbeI BbsI BciVI
BclI BlpI BmrI BsaAI BsaHI BseRI BseYI BsgI
BsmI Bsp1286I BspMI BsrDI BssHII BssSI BstAPI BtgI
BtsI EaeI EcoRV FspI HaeII KasI MscI MslI
MspA1I NarI NspI PasI PmlI PpiI PshAI PstI
ScaI SexAI SfcI SfoI StuI StyI TaqII TatI
TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PpuMI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI
XmnI ZraI