Restriction map for
pFN21AE1844
(Linear) (Six-base) MAPSORT of: KIEE1844.seq Check: 2267 from: 1 to: 1083
KIEE1844.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
Acc65I G'GTAC_C
Cuts at: 0 393 1083
Size: 393 690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 343 703 1083
Size: 280 63 360 380
Fragments arranged by size:
380 360 280 63
AhdI GACnn_n'nnGTC
Cuts at: 0 386 1083
Size: 386 697
AlwNI CAG_nnn'CTG
Cuts at: 0 140 624 1083
Size: 140 484 459
Fragments arranged by size:
484 459 140
ApaI G_GGCC'C
Cuts at: 0 875 1083
Size: 875 208
ApoI r'AATT_y
Cuts at: 0 340 1083
Size: 340 743
AvaI C'yCGr_G
Cuts at: 0 248 947 1083
Size: 248 699 136
Fragments arranged by size:
699 248 136
BamHI G'GATC_C
Cuts at: 0 26 1083
Size: 26 1057
BanI G'GyrC_C
Cuts at: 0 393 417 1083
Size: 393 24 666
Fragments arranged by size:
666 393 24
BanII G_rGCy'C
Cuts at: 0 875 1083
Size: 875 208
BbeI G_GCGC'C
Cuts at: 0 421 1083
Size: 421 662
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 1083
Size: 467 616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 629 1083
Size: 595 34 454
Fragments arranged by size:
595 454 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 14 1083
Size: 14 1069
BlpI GC'TnA_GC
Cuts at: 0 665 1083
Size: 665 418
Bme1580I G_kGCm'C
Cuts at: 0 875 1083
Size: 875 208
BmgBI CAC'GTC
Cuts at: 0 151 312 1083
Size: 151 161 771
Fragments arranged by size:
771 161 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 869 1083
Size: 394 475 214
Fragments arranged by size:
475 394 214
Bpu10I CC'TnA_GC
Cuts at: 0 31 1083
Size: 31 1052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 991 1083
Size: 823 168 92
Fragments arranged by size:
823 168 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 1032 1083
Size: 1032 51
BsaHI Gr'CG_yC
Cuts at: 0 418 1083
Size: 418 665
BsaWI w'CCGG_w
Cuts at: 0 84 390 396 1083
Size: 84 306 6 687
Fragments arranged by size:
687 306 84 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 515 545 1083
Size: 515 30 538
Fragments arranged by size:
538 515 30
BsiHKAI G_wGCw'C
Cuts at: 0 681 1083
Size: 681 402
BsmI GAATG_Cn'
Cuts at: 0 759 1083
Size: 759 324
Bsp1286I G_dGCh'C
Cuts at: 0 681 875 1083
Size: 681 194 208
Fragments arranged by size:
681 208 194
BsrBI CCG'CTC
Cuts at: 0 180 1083
Size: 180 903
BsrDI GCAATG_nn'
Cuts at: 0 334 1083
Size: 334 749
BstAPI GCAn_nnn'nTGC
Cuts at: 0 514 1083
Size: 514 569
BstXI CCAn_nnnn'nTGG
Cuts at: 0 907 1083
Size: 907 176
BstYI r'GATC_y
Cuts at: 0 26 351 409 1083
Size: 26 325 58 674
Fragments arranged by size:
674 325 58 26
BtgI C'CryG_G
Cuts at: 0 644 1083
Size: 644 439
BtsI GCAGTG_nn'
Cuts at: 0 515 1083
Size: 515 568
EaeI y'GGCC_r
Cuts at: 0 584 1083
Size: 584 499
EarI CTCTTCn'nnn_
Cuts at: 0 718 1083
Size: 718 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 343 703 1083
Size: 280 63 360 380
Fragments arranged by size:
380 360 280 63
EcoO109I rG'GnC_Cy
Cuts at: 0 871 1083
Size: 871 212
EcoRI G'AATT_C
Cuts at: 0 340 1083
Size: 340 743
HaeII r_GCGC'y
Cuts at: 0 16 421 1083
Size: 16 405 662
Fragments arranged by size:
662 405 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 178 184 188 210 216 372 404
Size: 156 22 6 4 22 6 156 32
Cuts at: 404 474 506 952 984 1083
Size: 70 32 446 32 99
Fragments arranged by size:
446 156 156 99 70 32 32 32
22 22 6 6 4
KasI G'GCGC_C
Cuts at: 0 417 1083
Size: 417 666
KpnI G_GTAC'C
Cuts at: 0 397 1083
Size: 397 686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1083
Size: 176 907
MscI TGG'CCA
Cuts at: 0 586 1083
Size: 586 497
NarI GG'CG_CC
Cuts at: 0 418 1083
Size: 418 665
NcoI C'CATG_G
Cuts at: 0 644 1083
Size: 644 439
NdeI CA'TA_TG
Cuts at: 0 746 1083
Size: 746 337
PspOMI G'GGCC_C
Cuts at: 0 871 1083
Size: 871 212
PstI C_TGCA'G
Cuts at: 0 602 629 1083
Size: 602 27 454
Fragments arranged by size:
602 454 27
SfcI C'TryA_G
Cuts at: 0 20 69 598 625 1083
Size: 20 49 529 27 458
Fragments arranged by size:
529 458 49 27 20
SfoI GGC'GCC
Cuts at: 0 419 1083
Size: 419 664
SmlI C'TyrA_G
Cuts at: 0 802 1006 1083
Size: 802 204 77
Fragments arranged by size:
802 204 77
StuI AGG'CCT
Cuts at: 0 269 1004 1014 1083
Size: 269 735 10 69
Fragments arranged by size:
735 269 69 10
StyI C'CwwG_G
Cuts at: 0 644 1083
Size: 644 439
XmnI GAAnn'nnTTC
Cuts at: 0 778 1083
Size: 778 305
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BlpI Bme1580I
BmgBI BmrI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BstAPI BstXI BstYI
BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRI HaeII
Hin4I KasI KpnI MmeI MscI NarI NcoI NdeI
PspOMI PstI SfcI SfoI SmlI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BclI BfrBI BglI BglII BmtI BplI
BpmI BsaAI BsaBI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MslI MspA1I NaeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI