Restriction map for
pFN21AE1837
(Linear) (Six-base) MAPSORT of: KIEE1837.seq Check: 2254 from: 1 to: 1062
KIEE1837.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 673 1016 1027 1062
Size: 673 343 11 35
Fragments arranged by size:
673 343 35 11
AccI GT'mk_AC
Cuts at: 0 570 1062
Size: 570 492
AfeI AGC'GCT
Cuts at: 0 1048 1062
Size: 1048 14
AleI CACnn'nnGTG
Cuts at: 0 205 578 1062
Size: 205 373 484
Fragments arranged by size:
484 373 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 1062
Size: 259 32 771
Fragments arranged by size:
771 259 32
AlwNI CAG_nnn'CTG
Cuts at: 0 600 687 1062
Size: 600 87 375
Fragments arranged by size:
600 375 87
ApaI G_GGCC'C
Cuts at: 0 449 1062
Size: 449 613
ApaLI G'TGCA_C
Cuts at: 0 208 742 1062
Size: 208 534 320
Fragments arranged by size:
534 320 208
AvaI C'yCGr_G
Cuts at: 0 323 380 442 448 1062
Size: 323 57 62 6 614
Fragments arranged by size:
614 323 62 57 6
BamHI G'GATC_C
Cuts at: 0 544 1062
Size: 544 518
BanI G'GyrC_C
Cuts at: 0 757 774 983 1062
Size: 757 17 209 79
Fragments arranged by size:
757 209 79 17
BanII G_rGCy'C
Cuts at: 0 194 287 449 494 835 871 1062
Size: 194 93 162 45 341 36 191
Fragments arranged by size:
341 194 191 162 93 45 36
BbvCI CC'TCA_GC
Cuts at: 0 481 1062
Size: 481 581
BglI GCCn_nnn'nGGC
Cuts at: 0 444 1062
Size: 444 618
Bme1580I G_kGCm'C
Cuts at: 0 212 449 746 760 779 1062
Size: 212 237 297 14 19 283
Fragments arranged by size:
297 283 237 212 19 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 614 1062
Size: 614 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 163 208 331 1062
Size: 34 129 45 123 731
Fragments arranged by size:
731 129 123 45 34
Bpu10I CC'TnA_GC
Cuts at: 0 481 1062
Size: 481 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1062
Size: 694 368
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 1062
Size: 259 30 773
Fragments arranged by size:
773 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 264 484 1062
Size: 264 220 578
Fragments arranged by size:
578 264 220
BseYI C'CCAG_C
Cuts at: 0 458 1062
Size: 458 604
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 187 372 1062
Size: 143 44 185 690
Fragments arranged by size:
690 185 143 44
BsiEI CG_ry'CG
Cuts at: 0 13 356 1062
Size: 13 343 706
Fragments arranged by size:
706 343 13
BsiHKAI G_wGCw'C
Cuts at: 0 194 212 287 746 835 1062
Size: 194 18 75 459 89 227
Fragments arranged by size:
459 227 194 89 75 18
Bsp1286I G_dGCh'C
Cuts at: 0 194 212 287 449 494 746 760 779
Size: 194 18 75 162 45 252 14 19
Cuts at: 779 835 871 1062
Size: 56 36 191
Fragments arranged by size:
252 194 191 162 75 56 45 36
19 18 14
BspHI T'CATG_A
Cuts at: 0 227 1062
Size: 227 835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 6 410 673 1016 1027 1062
Size: 6 404 263 343 11 35
Fragments arranged by size:
404 343 263 35 11 6
BsrBI CCG'CTC
Cuts at: 0 981 1062
Size: 981 81
BsrFI r'CCGG_y
Cuts at: 0 55 1062
Size: 55 1007
BssHII G'CGCG_C
Cuts at: 0 603 1062
Size: 603 459
BstAPI GCAn_nnn'nTGC
Cuts at: 0 687 774 1062
Size: 687 87 288
Fragments arranged by size:
687 288 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 1062
Size: 786 276
BstYI r'GATC_y
Cuts at: 0 544 1062
Size: 544 518
BtgI C'CryG_G
Cuts at: 0 633 752 873 1040 1062
Size: 633 119 121 167 22
Fragments arranged by size:
633 167 121 119 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 1062
Size: 360 702
BtsI GCAGTG_nn'
Cuts at: 0 129 898 1062
Size: 129 769 164
Fragments arranged by size:
769 164 129
DraIII CAC_nnn'GTG
Cuts at: 0 204 774 1062
Size: 204 570 288
Fragments arranged by size:
570 288 204
DrdI GACnn_nn'nnGTC
Cuts at: 0 327 973 1062
Size: 327 646 89
Fragments arranged by size:
646 327 89
EaeI y'GGCC_r
Cuts at: 0 10 353 728 1062
Size: 10 343 375 334
Fragments arranged by size:
375 343 334 10
EagI C'GGCC_G
Cuts at: 0 10 353 1062
Size: 10 343 709
Fragments arranged by size:
709 343 10
EarI CTCTTCn'nnn_
Cuts at: 0 40 1062
Size: 40 1022
EcoICRI GAG'CTC
Cuts at: 0 192 285 833 1062
Size: 192 93 548 229
Fragments arranged by size:
548 229 192 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 163 208 331 1062
Size: 34 129 45 123 731
Fragments arranged by size:
731 129 123 45 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1023 1062
Size: 1023 39
EcoO109I rG'GnC_Cy
Cuts at: 0 320 783 1062
Size: 320 463 279
Fragments arranged by size:
463 320 279
EcoRV GAT'ATC
Cuts at: 0 792 1062
Size: 792 270
HaeII r_GCGC'y
Cuts at: 0 31 49 88 1050 1062
Size: 31 18 39 962 12
Fragments arranged by size:
962 39 31 18 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1062
Size: 932 130
MslI CAynn'nnrTG
Cuts at: 0 205 578 1062
Size: 205 373 484
Fragments arranged by size:
484 373 205
MspA1I CmG'CkG
Cuts at: 0 141 171 458 923 1062
Size: 141 30 287 465 139
Fragments arranged by size:
465 287 141 139 30
NcoI C'CATG_G
Cuts at: 0 633 752 1062
Size: 633 119 310
Fragments arranged by size:
633 310 119
NotI GC'GGCC_GC
Cuts at: 0 10 1062
Size: 10 1052
NspI r_CATG'y
Cuts at: 0 261 1062
Size: 261 801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 1062
Size: 259 32 771
Fragments arranged by size:
771 259 32
PpuMI rG'GwC_Cy
Cuts at: 0 320 783 1062
Size: 320 463 279
Fragments arranged by size:
463 320 279
PspOMI G'GGCC_C
Cuts at: 0 445 1062
Size: 445 617
PstI C_TGCA'G
Cuts at: 0 683 692 830 1062
Size: 683 9 138 232
Fragments arranged by size:
683 232 138 9
PvuII CAG'CTG
Cuts at: 0 141 171 923 1062
Size: 141 30 752 139
Fragments arranged by size:
752 141 139 30
SacI G_AGCT'C
Cuts at: 0 194 287 835 1062
Size: 194 93 548 227
Fragments arranged by size:
548 227 194 93
SanDI GG'GwC_CC
Cuts at: 0 320 1062
Size: 320 742
SapI GCTCTTCn'nnn_
Cuts at: 0 40 1062
Size: 40 1022
SexAI A'CCwGG_T
Cuts at: 0 770 1062
Size: 770 292
SfcI C'TryA_G
Cuts at: 0 679 688 826 1062
Size: 679 9 138 236
Fragments arranged by size:
679 236 138 9
SmaI CCC'GGG
Cuts at: 0 325 382 444 450 1062
Size: 325 57 62 6 612
Fragments arranged by size:
612 325 62 57 6
SmlI C'TyrA_G
Cuts at: 0 709 1062
Size: 709 353
SrfI GCCC'GGGC
Cuts at: 0 382 1062
Size: 382 680
StuI AGG'CCT
Cuts at: 0 235 1062
Size: 235 827
StyI C'CwwG_G
Cuts at: 0 633 734 752 779 1062
Size: 633 101 18 27 283
Fragments arranged by size:
633 283 101 27 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 607 639 1062
Size: 607 32 423
Fragments arranged by size:
607 423 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 640 1062
Size: 640 422
XmaI C'CCGG_G
Cuts at: 0 323 380 442 448 1062
Size: 323 57 62 6 614
Fragments arranged by size:
614 323 62 57 6
Enzymes that do cut:
AarI AccI AfeI AleI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbvCI BglI Bme1580I BplI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII
MmeI MslI MspA1I NcoI NotI NspI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI
SmaI SmlI SrfI StuI StyI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI EciI EcoRI FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TaqII TatI Tth111I
XbaI XhoI XmnI ZraI