Restriction map for
pFN21AE1836
(Linear) (Six-base) MAPSORT of: KIEE1836.seq Check: 3473 from: 1 to: 1062
KIEE1836.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 940 1062
Size: 940 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1062
Size: 650 412
AflIII A'CryG_T
Cuts at: 0 413 1062
Size: 413 649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1020 1062
Size: 1020 42
AlwNI CAG_nnn'CTG
Cuts at: 0 526 1062
Size: 526 536
ApaI G_GGCC'C
Cuts at: 0 1043 1062
Size: 1043 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 506 539 1062
Size: 506 33 523
Fragments arranged by size:
523 506 33
BanII G_rGCy'C
Cuts at: 0 64 1043 1062
Size: 64 979 19
Fragments arranged by size:
979 64 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 1062
Size: 746 316
BbvCI CC'TCA_GC
Cuts at: 0 205 1062
Size: 205 857
BglII A'GATC_T
Cuts at: 0 837 1062
Size: 837 225
BlpI GC'TnA_GC
Cuts at: 0 240 1062
Size: 240 822
Bme1580I G_kGCm'C
Cuts at: 0 1043 1062
Size: 1043 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 739 910 1062
Size: 48 691 171 152
Fragments arranged by size:
691 171 152 48
Bpu10I CC'TnA_GC
Cuts at: 0 205 1062
Size: 205 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 886 1062
Size: 886 176
BseYI C'CCAG_C
Cuts at: 0 369 1062
Size: 369 693
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 351 1062
Size: 76 275 711
Fragments arranged by size:
711 275 76
Bsp1286I G_dGCh'C
Cuts at: 0 64 1043 1062
Size: 64 979 19
Fragments arranged by size:
979 64 19
BspHI T'CATG_A
Cuts at: 0 32 1062
Size: 32 1030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 904 940 1062
Size: 904 36 122
Fragments arranged by size:
904 122 36
BsrDI GCAATG_nn'
Cuts at: 0 444 1002 1062
Size: 444 558 60
Fragments arranged by size:
558 444 60
BsrGI T'GTAC_A
Cuts at: 0 2 1062
Size: 2 1060
BstBI TT'CG_AA
Cuts at: 0 162 1062
Size: 162 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 1062
Size: 369 693
BstYI r'GATC_y
Cuts at: 0 613 623 837 1062
Size: 613 10 214 225
Fragments arranged by size:
613 225 214 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 246 281 1062
Size: 246 35 781
Fragments arranged by size:
781 246 35
EaeI y'GGCC_r
Cuts at: 0 359 1062
Size: 359 703
EarI CTCTTCn'nnn_
Cuts at: 0 370 437 1062
Size: 370 67 625
Fragments arranged by size:
625 370 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 650 739 910 1062
Size: 48 602 89 171 152
Fragments arranged by size:
602 171 152 89 48
EcoO109I rG'GnC_Cy
Cuts at: 0 1039 1040 1062
Size: 1039 1 22
Fragments arranged by size:
1039 22 1
EcoRV GAT'ATC
Cuts at: 0 503 1062
Size: 503 559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 466 1062
Size: 466 596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 1062
Size: 223 32 807
Fragments arranged by size:
807 223 32
MscI TGG'CCA
Cuts at: 0 361 1062
Size: 361 701
MspA1I CmG'CkG
Cuts at: 0 51 938 1062
Size: 51 887 124
Fragments arranged by size:
887 124 51
NspI r_CATG'y
Cuts at: 0 678 995 1062
Size: 678 317 67
Fragments arranged by size:
678 317 67
PasI CC'CwG_GG
Cuts at: 0 1035 1062
Size: 1035 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 730 1062
Size: 730 332
PfoI T'CCnGG_A
Cuts at: 0 755 1062
Size: 755 307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 784 1062
Size: 752 32 278
Fragments arranged by size:
752 278 32
PspOMI G'GGCC_C
Cuts at: 0 1039 1062
Size: 1039 23
PstI C_TGCA'G
Cuts at: 0 865 937 1062
Size: 865 72 125
Fragments arranged by size:
865 125 72
PvuII CAG'CTG
Cuts at: 0 51 938 1062
Size: 51 887 124
Fragments arranged by size:
887 124 51
SfcI C'TryA_G
Cuts at: 0 861 933 1062
Size: 861 72 129
Fragments arranged by size:
861 129 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 242 938 1062
Size: 242 696 124
Fragments arranged by size:
696 242 124
TatI w'GTAC_w
Cuts at: 0 2 1062
Size: 2 1060
XmnI GAAnn'nnTTC
Cuts at: 0 28 158 415 1062
Size: 28 130 257 647
Fragments arranged by size:
647 257 130 28
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI BaeI BanII
BbsI BbvCI BglII BlpI Bme1580I BpmI Bpu10I BsaI
BseYI BsgI Bsp1286I BspHI BspMI BsrDI BsrGI BstBI
BstXI BstYI CspCI EaeI EarI Eco57MI EcoO109I EcoRV
FalI Hin4I MscI MspA1I NspI PasI PflMI PfoI
PpiI PspOMI PstI PvuII SfcI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI