Restriction map for
pFN21AE1833
(Linear) (Six-base) MAPSORT of: KIEE1833.seq Check: 6487 from: 1 to: 1053
KIEE1833.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 888 1053
Size: 888 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1053
Size: 193 860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 1053
Size: 148 32 873
Fragments arranged by size:
873 148 32
AlwNI CAG_nnn'CTG
Cuts at: 0 645 1035 1053
Size: 645 390 18
Fragments arranged by size:
645 390 18
ApaLI G'TGCA_C
Cuts at: 0 879 1053
Size: 879 174
ApoI r'AATT_y
Cuts at: 0 409 598 943 1053
Size: 409 189 345 110
Fragments arranged by size:
409 345 189 110
BanI G'GyrC_C
Cuts at: 0 136 705 872 1053
Size: 136 569 167 181
Fragments arranged by size:
569 181 167 136
BanII G_rGCy'C
Cuts at: 0 474 1053
Size: 474 579
BbeI G_GCGC'C
Cuts at: 0 876 1053
Size: 876 177
BbvCI CC'TCA_GC
Cuts at: 0 713 1053
Size: 713 340
Bme1580I G_kGCm'C
Cuts at: 0 141 710 883 1029 1053
Size: 141 569 173 146 24
Fragments arranged by size:
569 173 146 141 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 711 1023 1053
Size: 711 312 30
Fragments arranged by size:
711 312 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1053
Size: 302 751
Bpu10I CC'TnA_GC
Cuts at: 0 713 1053
Size: 713 340
BsaHI Gr'CG_yC
Cuts at: 0 873 1053
Size: 873 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 1053
Size: 148 30 875
Fragments arranged by size:
875 148 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 1053
Size: 169 884
BseYI C'CCAG_C
Cuts at: 0 679 1053
Size: 679 374
BsiHKAI G_wGCw'C
Cuts at: 0 883 1053
Size: 883 170
BsmI GAATG_Cn'
Cuts at: 0 49 1053
Size: 49 1004
Bsp1286I G_dGCh'C
Cuts at: 0 141 474 710 883 1029 1053
Size: 141 333 236 173 146 24
Fragments arranged by size:
333 236 173 146 141 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 787 888 1053
Size: 787 101 165
Fragments arranged by size:
787 165 101
BsrFI r'CCGG_y
Cuts at: 0 499 1053
Size: 499 554
BsrGI T'GTAC_A
Cuts at: 0 290 1053
Size: 290 763
BstAPI GCAn_nnn'nTGC
Cuts at: 0 55 1053
Size: 55 998
BstBI TT'CG_AA
Cuts at: 0 326 1053
Size: 326 727
BstYI r'GATC_y
Cuts at: 0 380 422 1053
Size: 380 42 631
Fragments arranged by size:
631 380 42
BtgI C'CryG_G
Cuts at: 0 372 1053
Size: 372 681
BtsI GCAGTG_nn'
Cuts at: 0 518 1029 1053
Size: 518 511 24
Fragments arranged by size:
518 511 24
DraIII CAC_nnn'GTG
Cuts at: 0 705 887 1053
Size: 705 182 166
Fragments arranged by size:
705 182 166
EarI CTCTTCn'nnn_
Cuts at: 0 212 899 1053
Size: 212 687 154
Fragments arranged by size:
687 212 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 754 1053
Size: 754 299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 302 1053
Size: 193 109 751
Fragments arranged by size:
751 193 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1039 1053
Size: 1039 14
EcoO109I rG'GnC_Cy
Cuts at: 0 94 1053
Size: 94 959
EcoRI G'AATT_C
Cuts at: 0 598 1053
Size: 598 455
FspI TGC'GCA
Cuts at: 0 1008 1053
Size: 1008 45
HaeII r_GCGC'y
Cuts at: 0 646 692 876 1053
Size: 646 46 184 177
Fragments arranged by size:
646 184 177 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 1053
Size: 78 32 943
Fragments arranged by size:
943 78 32
KasI G'GCGC_C
Cuts at: 0 872 1053
Size: 872 181
MslI CAynn'nnrTG
Cuts at: 0 540 849 1053
Size: 540 309 204
Fragments arranged by size:
540 309 204
MspA1I CmG'CkG
Cuts at: 0 591 672 712 932 1053
Size: 591 81 40 220 121
Fragments arranged by size:
591 220 121 81 40
NaeI GCC'GGC
Cuts at: 0 501 1053
Size: 501 552
NarI GG'CG_CC
Cuts at: 0 873 1053
Size: 873 180
NcoI C'CATG_G
Cuts at: 0 372 1053
Size: 372 681
NdeI CA'TA_TG
Cuts at: 0 37 1053
Size: 37 1016
NgoMIV G'CCGG_C
Cuts at: 0 499 1053
Size: 499 554
NspI r_CATG'y
Cuts at: 0 268 539 581 988 1053
Size: 268 271 42 407 65
Fragments arranged by size:
407 271 268 65 42
PasI CC'CwG_GG
Cuts at: 0 1035 1053
Size: 1035 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1053
Size: 1035 18
PfoI T'CCnGG_A
Cuts at: 0 602 1053
Size: 602 451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 1053
Size: 148 32 873
Fragments arranged by size:
873 148 32
PpuMI rG'GwC_Cy
Cuts at: 0 94 1053
Size: 94 959
PstI C_TGCA'G
Cuts at: 0 397 568 590 899 1053
Size: 397 171 22 309 154
Fragments arranged by size:
397 309 171 154 22
PvuII CAG'CTG
Cuts at: 0 591 672 932 1053
Size: 591 81 260 121
Fragments arranged by size:
591 260 121 81
SapI GCTCTTCn'nnn_
Cuts at: 0 212 1053
Size: 212 841
SfcI C'TryA_G
Cuts at: 0 309 393 564 586 826 895 1053
Size: 309 84 171 22 240 69 158
Fragments arranged by size:
309 240 171 158 84 69 22
SfoI GGC'GCC
Cuts at: 0 874 1053
Size: 874 179
SpeI A'CTAG_T
Cuts at: 0 121 1053
Size: 121 932
StuI AGG'CCT
Cuts at: 0 103 718 1053
Size: 103 615 335
Fragments arranged by size:
615 335 103
StyI C'CwwG_G
Cuts at: 0 98 131 372 1053
Size: 98 33 241 681
Fragments arranged by size:
681 241 98 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 235 307 693 1053
Size: 124 111 72 386 360
Fragments arranged by size:
386 360 124 111 72
TatI w'GTAC_w
Cuts at: 0 290 1053
Size: 290 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 829 861 1053
Size: 829 32 192
Fragments arranged by size:
829 192 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 1053
Size: 435 618
XmnI GAAnn'nnTTC
Cuts at: 0 79 1053
Size: 79 974
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaLI ApoI BanI BanII
BbeI BbvCI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI
BstAPI BstBI BstYI BtgI BtsI DraIII EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PflMI PfoI PpiI PpuMI PstI PvuII SapI
SfcI SfoI SpeI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI
EcoICRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI