Restriction map for
pFN21AE1832
(Linear) (Six-base) MAPSORT of: KIEE1832.seq Check: 1558 from: 1 to: 1053
KIEE1832.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 343 945 1053
Size: 147 196 602 108
Fragments arranged by size:
602 196 147 108
AatII G_ACGT'C
Cuts at: 0 898 1053
Size: 898 155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1053
Size: 721 332
AflIII A'CryG_T
Cuts at: 0 1043 1053
Size: 1043 10
AleI CACnn'nnGTG
Cuts at: 0 727 1053
Size: 727 326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 1053
Size: 877 176
ApoI r'AATT_y
Cuts at: 0 985 1053
Size: 985 68
AvaI C'yCGr_G
Cuts at: 0 317 1053
Size: 317 736
BanI G'GyrC_C
Cuts at: 0 208 718 765 1053
Size: 208 510 47 288
Fragments arranged by size:
510 288 208 47
BanII G_rGCy'C
Cuts at: 0 552 751 1053
Size: 552 199 302
Fragments arranged by size:
552 302 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 33 42 282 1053
Size: 33 9 240 771
Fragments arranged by size:
771 240 33 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 332 1053
Size: 298 34 721
Fragments arranged by size:
721 298 34
BglI GCCn_nnn'nGGC
Cuts at: 0 217 443 746 1053
Size: 217 226 303 307
Fragments arranged by size:
307 303 226 217
Bme1580I G_kGCm'C
Cuts at: 0 213 762 871 1053
Size: 213 549 109 182
Fragments arranged by size:
549 213 182 109
BmgBI CAC'GTC
Cuts at: 0 125 656 1053
Size: 125 531 397
Fragments arranged by size:
531 397 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 738 1053
Size: 418 320 315
Fragments arranged by size:
418 320 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1053
Size: 121 932
BsaAI yAC'GTr
Cuts at: 0 1046 1053
Size: 1046 7
BsaHI Gr'CG_yC
Cuts at: 0 895 1053
Size: 895 158
BsaWI w'CCGG_w
Cuts at: 0 245 1053
Size: 245 808
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 220 250 1053
Size: 109 30 81 30 803
Fragments arranged by size:
803 109 81 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 130 133 241 741 1053
Size: 25 105 3 108 500 312
Fragments arranged by size:
500 312 108 105 25 3
BseYI C'CCAG_C
Cuts at: 0 212 864 1053
Size: 212 652 189
Fragments arranged by size:
652 212 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 402 1053
Size: 181 221 651
Fragments arranged by size:
651 221 181
BsmI GAATG_Cn'
Cuts at: 0 619 1053
Size: 619 434
BsmBI CGTCTCn'nnnn_
Cuts at: 0 115 1053
Size: 115 938
Bsp1286I G_dGCh'C
Cuts at: 0 213 552 751 762 871 1053
Size: 213 339 199 11 109 182
Fragments arranged by size:
339 213 199 182 109 11
BspEI T'CCGG_A
Cuts at: 0 245 1053
Size: 245 808
BspHI T'CATG_A
Cuts at: 0 554 1053
Size: 554 499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 343 945 1053
Size: 147 196 602 108
Fragments arranged by size:
602 196 147 108
BsrBI CCG'CTC
Cuts at: 0 11 1053
Size: 11 1042
BsrFI r'CCGG_y
Cuts at: 0 251 577 1013 1053
Size: 251 326 436 40
Fragments arranged by size:
436 326 251 40
BssSI C'ACGA_G
Cuts at: 0 12 870 1030 1053
Size: 12 858 160 23
Fragments arranged by size:
858 160 23 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 849 1053
Size: 849 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 762 1053
Size: 729 33 291
Fragments arranged by size:
729 291 33
BtgI C'CryG_G
Cuts at: 0 204 624 936 1053
Size: 204 420 312 117
Fragments arranged by size:
420 312 204 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 459 1053
Size: 459 594
BtsI GCAGTG_nn'
Cuts at: 0 374 393 1024 1053
Size: 374 19 631 29
Fragments arranged by size:
631 374 29 19
DraIII CAC_nnn'GTG
Cuts at: 0 630 1053
Size: 630 423
EaeI y'GGCC_r
Cuts at: 0 83 218 933 1053
Size: 83 135 715 120
Fragments arranged by size:
715 135 120 83
EarI CTCTTCn'nnn_
Cuts at: 0 351 1053
Size: 351 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 670 1053
Size: 670 383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 721 738 1053
Size: 418 303 17 315
Fragments arranged by size:
418 315 303 17
EcoO109I rG'GnC_Cy
Cuts at: 0 141 533 804 1053
Size: 141 392 271 249
Fragments arranged by size:
392 271 249 141
FspI TGC'GCA
Cuts at: 0 853 1053
Size: 853 200
HaeII r_GCGC'y
Cuts at: 0 258 1053
Size: 258 795
MscI TGG'CCA
Cuts at: 0 85 220 1053
Size: 85 135 833
Fragments arranged by size:
833 135 85
MslI CAynn'nnrTG
Cuts at: 0 569 607 727 797 1053
Size: 569 38 120 70 256
Fragments arranged by size:
569 256 120 70 38
MspA1I CmG'CkG
Cuts at: 0 206 216 857 938 980 1026 1053
Size: 206 10 641 81 42 46 27
Fragments arranged by size:
641 206 81 46 42 27 10
NaeI GCC'GGC
Cuts at: 0 579 1015 1053
Size: 579 436 38
Fragments arranged by size:
579 436 38
NgoMIV G'CCGG_C
Cuts at: 0 577 1013 1053
Size: 577 436 40
Fragments arranged by size:
577 436 40
NspI r_CATG'y
Cuts at: 0 606 852 887 1053
Size: 606 246 35 166
Fragments arranged by size:
606 246 166 35
PmlI CAC'GTG
Cuts at: 0 1046 1053
Size: 1046 7
PpuMI rG'GwC_Cy
Cuts at: 0 804 1053
Size: 804 249
PstI C_TGCA'G
Cuts at: 0 979 1053
Size: 979 74
PvuII CAG'CTG
Cuts at: 0 216 980 1053
Size: 216 764 73
Fragments arranged by size:
764 216 73
SacII CC_GC'GG
Cuts at: 0 207 939 1053
Size: 207 732 114
Fragments arranged by size:
732 207 114
SexAI A'CCwGG_T
Cuts at: 0 806 1053
Size: 806 247
SfcI C'TryA_G
Cuts at: 0 975 1053
Size: 975 78
SmlI C'TyrA_G
Cuts at: 0 136 1053
Size: 136 917
StyI C'CwwG_G
Cuts at: 0 899 1053
Size: 899 154
TatI w'GTAC_w
Cuts at: 0 812 1053
Size: 812 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 431 463 604 636 1053
Size: 109 32 290 32 141 32 417
Fragments arranged by size:
417 290 141 109 32 32 32
ZraI GAC'GTC
Cuts at: 0 896 1053
Size: 896 157
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI ApoI AvaI
BanI BanII BbsI BcgI BglI Bme1580I BmgBI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstXI BtgI BtgZI BtsI DraIII EaeI
EarI EciI Eco57MI EcoO109I FspI HaeII MscI MslI
MspA1I NaeI NgoMIV NspI PmlI PpuMI PstI PvuII
SacII SexAI SfcI SmlI StyI TatI TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI Bpu10I BsaI BsaBI BsiEI BsiHKAI
BsiWI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI XmnI