(Linear) (Six-base) MAPSORT of: KIEE1825.seq  Check: 2221  from: 1  to: 1023



KIEE1825.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     492     527     709    1023 
   Size:         76     416      35     182     314

  Fragments arranged by size: 

                 416     314     182      76      35
 
AlwNI CAG_nnn'CTG

Cuts at:      0     687    1023 
   Size:        687     336
 
ApoI r'AATT_y

Cuts at:      0     155     299     601    1023 
   Size:        155     144     302     422

  Fragments arranged by size: 

                 422     302     155     144
 
AseI AT'TA_AT

Cuts at:      0     772    1023 
   Size:        772     251
 
BanII G_rGCy'C

Cuts at:      0     236    1023 
   Size:        236     787
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     327     973    1023 
   Size:        327     646      50

  Fragments arranged by size: 

                 646     327      50
 
BbvCI CC'TCA_GC

Cuts at:      0     916    1023 
   Size:        916     107
 
BfrBI ATG'CAT

Cuts at:      0     449     550    1023 
   Size:        449     101     473

  Fragments arranged by size: 

                 473     449     101
 
BlpI GC'TnA_GC

Cuts at:      0    1010    1023 
   Size:       1010      13
 
BmgBI CAC'GTC

Cuts at:      0     291    1023 
   Size:        291     732
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     392    1023 
   Size:         17     375     631

  Fragments arranged by size: 

                 631     375      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     238     916    1023 
   Size:        238     678     107

  Fragments arranged by size: 

                 678     238     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197     871    1023 
   Size:        197     674     152

  Fragments arranged by size: 

                 674     197     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     996    1023 
   Size:        996      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     324     354    1023 
   Size:        324      30     669

  Fragments arranged by size: 

                 669     324      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     874    1023 
   Size:        874     149
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     617    1023 
   Size:        345     272     406

  Fragments arranged by size: 

                 406     345     272
 
BsmI GAATG_Cn'

Cuts at:      0     451    1023 
   Size:        451     572
 
Bsp1286I G_dGCh'C

Cuts at:      0     236    1023 
   Size:        236     787
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     791    1023 
   Size:        791     232
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     138    1023 
   Size:        138     885
 
EarI CTCTTCn'nnn_

Cuts at:      0     994    1023 
   Size:        994      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17      76     392     492     527     709    1023 
   Size:         17      59     316     100      35     182     314

  Fragments arranged by size: 

                 316     314     182     100      59      35      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     434     466    1023 
   Size:        434      32     557

  Fragments arranged by size: 

                 557     434      32
 
HincII GTy'rAC

Cuts at:      0     527    1023 
   Size:        527     496
 
HindIII A'AGCT_T

Cuts at:      0     691    1023 
   Size:        691     332
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    1023 
   Size:        467     556
 
MslI CAynn'nnrTG

Cuts at:      0     733    1023 
   Size:        733     290
 
NsiI A_TGCA'T

Cuts at:      0     451     552    1023 
   Size:        451     101     471

  Fragments arranged by size: 

                 471     451     101
 
PshAI GACnn'nnGTC

Cuts at:      0     797    1023 
   Size:        797     226
 
SfcI C'TryA_G

Cuts at:      0     116    1023 
   Size:        116     907
 
SmlI C'TyrA_G

Cuts at:      0     212     886    1023 
   Size:        212     674     137

  Fragments arranged by size: 

                 674     212     137
 
SspI AAT'ATT

Cuts at:      0     312     375    1023 
   Size:        312      63     648

  Fragments arranged by size: 

                 648     312      63
 
StuI AGG'CCT

Cuts at:      0     820    1023 
   Size:        820     203
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     379    1023 
   Size:        379     644
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AseI    BanII     BbsI    BbvCI    BfrBI
     BlpI    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI
     BsgI     BsmI Bsp1286I    BspMI    BstXI     EarI  Eco57MI    Hin4I
   HincII  HindIII     MmeI     MslI     NsiI    PshAI     SfcI     SmlI
     SspI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI     BglI    BglII Bme1580I     BmrI     BmtI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI