Restriction map for
pFN21AE1822
(Linear) (Six-base) MAPSORT of: KIEE1822.seq Check: 263 from: 1 to: 1005
KIEE1822.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 556 1005
Size: 556 449
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 223 616 1005
Size: 77 146 393 389
Fragments arranged by size:
393 389 146 77
AfeI AGC'GCT
Cuts at: 0 54 1005
Size: 54 951
ApaLI G'TGCA_C
Cuts at: 0 364 1005
Size: 364 641
ApoI r'AATT_y
Cuts at: 0 301 1005
Size: 301 704
BanI G'GyrC_C
Cuts at: 0 5 544 1005
Size: 5 539 461
Fragments arranged by size:
539 461 5
BanII G_rGCy'C
Cuts at: 0 341 1005
Size: 341 664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 278 1005
Size: 244 34 727
Fragments arranged by size:
727 244 34
BglII A'GATC_T
Cuts at: 0 95 1005
Size: 95 910
Bme1580I G_kGCm'C
Cuts at: 0 223 368 742 1005
Size: 223 145 374 263
Fragments arranged by size:
374 263 223 145
BmgBI CAC'GTC
Cuts at: 0 294 1005
Size: 294 711
BmrI ACTGGGnnnn_n'
Cuts at: 0 632 891 1005
Size: 632 259 114
Fragments arranged by size:
632 259 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 76 192 325 1005
Size: 22 54 116 133 680
Fragments arranged by size:
680 133 116 54 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1005
Size: 595 410
BsaHI Gr'CG_yC
Cuts at: 0 998 1005
Size: 998 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 937 1005
Size: 25 912 68
Fragments arranged by size:
912 68 25
BseYI C'CCAG_C
Cuts at: 0 147 811 1005
Size: 147 664 194
Fragments arranged by size:
664 194 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1005
Size: 547 458
BsiHKAI G_wGCw'C
Cuts at: 0 341 368 497 1005
Size: 341 27 129 508
Fragments arranged by size:
508 341 129 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 238 1005
Size: 238 767
Bsp1286I G_dGCh'C
Cuts at: 0 223 341 368 497 742 1005
Size: 223 118 27 129 245 263
Fragments arranged by size:
263 245 223 129 118 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 556 1005
Size: 556 449
BsrBI CCG'CTC
Cuts at: 0 197 1005
Size: 197 808
BstYI r'GATC_y
Cuts at: 0 95 1005
Size: 95 910
BtgI C'CryG_G
Cuts at: 0 630 902 1005
Size: 630 272 103
Fragments arranged by size:
630 272 103
BtsI GCAGTG_nn'
Cuts at: 0 244 535 1005
Size: 244 291 470
Fragments arranged by size:
470 291 244
EaeI y'GGCC_r
Cuts at: 0 482 651 1005
Size: 482 169 354
Fragments arranged by size:
482 354 169
EcoICRI GAG'CTC
Cuts at: 0 339 1005
Size: 339 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 76 77 192 223 325 616 1005
Size: 22 54 1 115 31 102 291 389
Fragments arranged by size:
389 291 115 102 54 31 22 1
EcoO109I rG'GnC_Cy
Cuts at: 0 462 1005
Size: 462 543
EcoRI G'AATT_C
Cuts at: 0 301 1005
Size: 301 704
EcoRV GAT'ATC
Cuts at: 0 420 1005
Size: 420 585
HaeII r_GCGC'y
Cuts at: 0 34 56 1005
Size: 34 22 949
Fragments arranged by size:
949 34 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 768 800 1005
Size: 768 32 205
Fragments arranged by size:
768 205 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1005
Size: 569 436
MscI TGG'CCA
Cuts at: 0 484 1005
Size: 484 521
MslI CAynn'nnrTG
Cuts at: 0 490 901 1005
Size: 490 411 104
Fragments arranged by size:
490 411 104
MspA1I CmG'CkG
Cuts at: 0 86 119 170 352 823 1005
Size: 86 33 51 182 471 182
Fragments arranged by size:
471 182 182 86 51 33
NcoI C'CATG_G
Cuts at: 0 902 1005
Size: 902 103
PasI CC'CwG_GG
Cuts at: 0 216 370 1005
Size: 216 154 635
Fragments arranged by size:
635 216 154
PflMI CCAn_nnn'nTGG
Cuts at: 0 620 1005
Size: 620 385
PstI C_TGCA'G
Cuts at: 0 161 254 1005
Size: 161 93 751
Fragments arranged by size:
751 161 93
PvuII CAG'CTG
Cuts at: 0 119 170 823 1005
Size: 119 51 653 182
Fragments arranged by size:
653 182 119 51
SacI G_AGCT'C
Cuts at: 0 341 1005
Size: 341 664
SfcI C'TryA_G
Cuts at: 0 157 250 1005
Size: 157 93 755
Fragments arranged by size:
755 157 93
SmlI C'TyrA_G
Cuts at: 0 574 1005
Size: 574 431
StuI AGG'CCT
Cuts at: 0 619 1005
Size: 619 386
StyI C'CwwG_G
Cuts at: 0 208 332 432 614 902 917 1005
Size: 208 124 100 182 288 15 88
Fragments arranged by size:
288 208 182 124 100 88 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 693 1005
Size: 693 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 1005
Size: 705 32 268
Fragments arranged by size:
705 268 32
Tth111I GACn'n_nGTC
Cuts at: 0 190 871 1005
Size: 190 681 134
Fragments arranged by size:
681 190 134
XbaI T'CTAG_A
Cuts at: 0 855 1005
Size: 855 150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 562 698 1005
Size: 349 213 136 307
Fragments arranged by size:
349 307 213 136
XmnI GAAnn'nnTTC
Cuts at: 0 301 1005
Size: 301 704
Enzymes that do cut:
AarI AcuI AfeI ApaLI ApoI BanI BanII BcgI
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaHI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstYI
BtgI BtsI EaeI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I MmeI MscI MslI MspA1I NcoI PasI
PflMI PstI PvuII SacI SfcI SmlI StuI StyI
TaqII TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoNI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI XhoI XmaI ZraI