Restriction map for
pFN21AE1812
(Linear) (Six-base) MAPSORT of: KIEE1812.seq Check: 8864 from: 1 to: 984
KIEE1812.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 430 741 984
Size: 430 311 243
Fragments arranged by size:
430 311 243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 179 748 984
Size: 97 82 569 236
Fragments arranged by size:
569 236 97 82
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 984
Size: 937 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 984
Size: 269 32 683
Fragments arranged by size:
683 269 32
AlwNI CAG_nnn'CTG
Cuts at: 0 593 604 984
Size: 593 11 380
Fragments arranged by size:
593 380 11
ApaI G_GGCC'C
Cuts at: 0 930 984
Size: 930 54
ApoI r'AATT_y
Cuts at: 0 329 741 984
Size: 329 412 243
Fragments arranged by size:
412 329 243
BanII G_rGCy'C
Cuts at: 0 930 984
Size: 930 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 858 984
Size: 858 126
BbvCI CC'TCA_GC
Cuts at: 0 605 882 984
Size: 605 277 102
Fragments arranged by size:
605 277 102
BciVI GTATCCnnnnn_n'
Cuts at: 0 122 984
Size: 122 862
Bme1580I G_kGCm'C
Cuts at: 0 930 977 984
Size: 930 47 7
Fragments arranged by size:
930 47 7
BmgBI CAC'GTC
Cuts at: 0 506 984
Size: 506 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 804 984
Size: 146 658 180
Fragments arranged by size:
658 180 146
Bpu10I CC'TnA_GC
Cuts at: 0 605 882 984
Size: 605 277 102
Fragments arranged by size:
605 277 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 984
Size: 637 347
BsaBI GATnn'nnATC
Cuts at: 0 110 984
Size: 110 874
BsaWI w'CCGG_w
Cuts at: 0 114 984
Size: 114 870
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 119 149 269 299 547 577 984
Size: 119 30 120 30 248 30 407
Fragments arranged by size:
407 248 120 119 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 984
Size: 622 362
BseYI C'CCAG_C
Cuts at: 0 597 764 984
Size: 597 167 220
Fragments arranged by size:
597 220 167
Bsp1286I G_dGCh'C
Cuts at: 0 930 977 984
Size: 930 47 7
Fragments arranged by size:
930 47 7
BspEI T'CCGG_A
Cuts at: 0 114 984
Size: 114 870
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 430 741 984
Size: 430 311 243
Fragments arranged by size:
430 311 243
BssSI C'ACGA_G
Cuts at: 0 58 984
Size: 58 926
BtgI C'CryG_G
Cuts at: 0 887 984
Size: 887 97
BtsI GCAGTG_nn'
Cuts at: 0 201 984
Size: 201 783
DraI TTT'AAA
Cuts at: 0 350 984
Size: 350 634
EaeI y'GGCC_r
Cuts at: 0 62 255 984
Size: 62 193 729
Fragments arranged by size:
729 193 62
EarI CTCTTCn'nnn_
Cuts at: 0 489 984
Size: 489 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 146 179 748 804 984
Size: 97 49 33 569 56 180
Fragments arranged by size:
569 180 97 56 49 33
EcoRI G'AATT_C
Cuts at: 0 741 984
Size: 741 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 984
Size: 865 32 87
Fragments arranged by size:
865 87 32
MscI TGG'CCA
Cuts at: 0 64 257 984
Size: 64 193 727
Fragments arranged by size:
727 193 64
MslI CAynn'nnrTG
Cuts at: 0 919 984
Size: 919 65
MspA1I CmG'CkG
Cuts at: 0 601 726 764 984
Size: 601 125 38 220
Fragments arranged by size:
601 220 125 38
NdeI CA'TA_TG
Cuts at: 0 67 984
Size: 67 917
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 984
Size: 15 969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 984
Size: 269 32 683
Fragments arranged by size:
683 269 32
PspOMI G'GGCC_C
Cuts at: 0 926 984
Size: 926 58
PstI C_TGCA'G
Cuts at: 0 531 982 984
Size: 531 451 2
Fragments arranged by size:
531 451 2
PvuII CAG'CTG
Cuts at: 0 601 726 764 984
Size: 601 125 38 220
Fragments arranged by size:
601 220 125 38
SapI GCTCTTCn'nnn_
Cuts at: 0 489 984
Size: 489 495
ScaI AGT'ACT
Cuts at: 0 447 565 789 984
Size: 447 118 224 195
Fragments arranged by size:
447 224 195 118
SfcI C'TryA_G
Cuts at: 0 527 942 978 984
Size: 527 415 36 6
Fragments arranged by size:
527 415 36 6
SmlI C'TyrA_G
Cuts at: 0 652 984
Size: 652 332
StyI C'CwwG_G
Cuts at: 0 182 495 984
Size: 182 313 489
Fragments arranged by size:
489 313 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 726 984
Size: 726 258
TatI w'GTAC_w
Cuts at: 0 260 445 563 787 984
Size: 260 185 118 224 197
Fragments arranged by size:
260 224 197 185 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 984
Size: 119 32 833
Fragments arranged by size:
833 119 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 627 984
Size: 627 357
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApoI BanII
BbsI BbvCI BciVI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaBI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspMI
BssSI BtgI BtsI DraI EaeI EarI Eco57MI EcoRI
Hin4I MscI MslI MspA1I NdeI PflMI PpiI PspOMI
PstI PvuII SapI ScaI SfcI SmlI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI BsaI BsaAI BsaHI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI