Restriction map for
pFN21AE1805
(Linear) (Six-base) MAPSORT of: KIEE1805.seq Check: 7690 from: 1 to: 960
KIEE1805.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 73 960
Size: 73 887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 960
Size: 420 540
AflII C'TTAA_G
Cuts at: 0 955 960
Size: 955 5
ApaLI G'TGCA_C
Cuts at: 0 88 454 960
Size: 88 366 506
Fragments arranged by size:
506 366 88
ApoI r'AATT_y
Cuts at: 0 324 592 960
Size: 324 268 368
Fragments arranged by size:
368 324 268
AscI GG'CGCG_CC
Cuts at: 0 38 960
Size: 38 922
BanII G_rGCy'C
Cuts at: 0 293 799 960
Size: 293 506 161
Fragments arranged by size:
506 293 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 586 960
Size: 586 374
BclI T'GATC_A
Cuts at: 0 368 960
Size: 368 592
Bme1580I G_kGCm'C
Cuts at: 0 92 458 616 960
Size: 92 366 158 344
Fragments arranged by size:
366 344 158 92
BmgBI CAC'GTC
Cuts at: 0 834 960
Size: 834 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 960
Size: 838 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 481 931 960
Size: 184 297 450 29
Fragments arranged by size:
450 297 184 29
BsaAI yAC'GTr
Cuts at: 0 129 960
Size: 129 831
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 448 478 960
Size: 370 30 48 30 482
Fragments arranged by size:
482 370 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 960
Size: 456 504
BseYI C'CCAG_C
Cuts at: 0 108 546 704 808 960
Size: 108 438 158 104 152
Fragments arranged by size:
438 158 152 108 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 397 916 960
Size: 151 246 519 44
Fragments arranged by size:
519 246 151 44
BsiHKAI G_wGCw'C
Cuts at: 0 92 434 458 960
Size: 92 342 24 502
Fragments arranged by size:
502 342 92 24
Bsp1286I G_dGCh'C
Cuts at: 0 92 293 434 458 616 799 960
Size: 92 201 141 24 158 183 161
Fragments arranged by size:
201 183 161 158 141 92 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 73 960
Size: 73 887
BsrBI CCG'CTC
Cuts at: 0 770 960
Size: 770 190
BsrDI GCAATG_nn'
Cuts at: 0 635 960
Size: 635 325
BsrFI r'CCGG_y
Cuts at: 0 74 960
Size: 74 886
BssHII G'CGCG_C
Cuts at: 0 38 960
Size: 38 922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 420 960
Size: 420 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 315 960
Size: 315 645
BtgI C'CryG_G
Cuts at: 0 449 960
Size: 449 511
BtsI GCAGTG_nn'
Cuts at: 0 879 960
Size: 879 81
DraI TTT'AAA
Cuts at: 0 188 960
Size: 188 772
EaeI y'GGCC_r
Cuts at: 0 123 663 960
Size: 123 540 297
Fragments arranged by size:
540 297 123
EarI CTCTTCn'nnn_
Cuts at: 0 131 787 960
Size: 131 656 173
Fragments arranged by size:
656 173 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 838 960
Size: 420 418 122
Fragments arranged by size:
420 418 122
EcoO109I rG'GnC_Cy
Cuts at: 0 441 960
Size: 441 519
EcoRI G'AATT_C
Cuts at: 0 324 960
Size: 324 636
HaeII r_GCGC'y
Cuts at: 0 115 122 355 960
Size: 115 7 233 605
Fragments arranged by size:
605 233 115 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 960
Size: 211 32 717
Fragments arranged by size:
717 211 32
HincII GTy'rAC
Cuts at: 0 684 960
Size: 684 276
MscI TGG'CCA
Cuts at: 0 125 665 960
Size: 125 540 295
Fragments arranged by size:
540 295 125
MslI CAynn'nnrTG
Cuts at: 0 67 960
Size: 67 893
MspA1I CmG'CkG
Cuts at: 0 546 760 960
Size: 546 214 200
Fragments arranged by size:
546 214 200
NspI r_CATG'y
Cuts at: 0 72 960
Size: 72 888
PmlI CAC'GTG
Cuts at: 0 129 960
Size: 129 831
PpuMI rG'GwC_Cy
Cuts at: 0 441 960
Size: 441 519
PstI C_TGCA'G
Cuts at: 0 216 874 960
Size: 216 658 86
Fragments arranged by size:
658 216 86
PvuII CAG'CTG
Cuts at: 0 546 960
Size: 546 414
SapI GCTCTTCn'nnn_
Cuts at: 0 787 960
Size: 787 173
SexAI A'CCwGG_T
Cuts at: 0 11 960
Size: 11 949
SfcI C'TryA_G
Cuts at: 0 212 870 960
Size: 212 658 90
Fragments arranged by size:
658 212 90
SmlI C'TyrA_G
Cuts at: 0 163 496 946 955 960
Size: 163 333 450 9 5
Fragments arranged by size:
450 333 163 9 5
StyI C'CwwG_G
Cuts at: 0 799 960
Size: 799 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 459 960
Size: 459 501
TatI w'GTAC_w
Cuts at: 0 849 960
Size: 849 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 448 480 960
Size: 448 32 480
Fragments arranged by size:
480 448 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 960
Size: 312 648
XmnI GAAnn'nnTTC
Cuts at: 0 491 960
Size: 491 469
Enzymes that do cut:
AarI AcuI AflII ApaLI ApoI AscI BanII BbsI
BclI Bme1580I BmgBI BpmI BpuEI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstXI BtgI BtsI DraI EaeI EarI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII MscI MslI
MspA1I NspI PmlI PpuMI PstI PvuII SapI SexAI
SfcI SmlI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I
BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI