Restriction map for
pFN21AE1804
(Linear) (Six-base) MAPSORT of: KIEE1804.seq Check: 3493 from: 1 to: 954
KIEE1804.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 432 954
Size: 432 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 954
Size: 769 185
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 745 777 954
Size: 745 32 177
Fragments arranged by size:
745 177 32
AlwNI CAG_nnn'CTG
Cuts at: 0 42 54 954
Size: 42 12 900
Fragments arranged by size:
900 42 12
ApoI r'AATT_y
Cuts at: 0 629 954
Size: 629 325
AseI AT'TA_AT
Cuts at: 0 779 954
Size: 779 175
AvaI C'yCGr_G
Cuts at: 0 178 954
Size: 178 776
BamHI G'GATC_C
Cuts at: 0 240 954
Size: 240 714
BanI G'GyrC_C
Cuts at: 0 112 954
Size: 112 842
BbsI GAAGACnn'nnnn_
Cuts at: 0 488 954
Size: 488 466
BfrBI ATG'CAT
Cuts at: 0 681 954
Size: 681 273
BglII A'GATC_T
Cuts at: 0 668 954
Size: 668 286
Bme1580I G_kGCm'C
Cuts at: 0 115 954
Size: 115 839
BmgBI CAC'GTC
Cuts at: 0 564 954
Size: 564 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 625 954
Size: 19 606 329
Fragments arranged by size:
606 329 19
Bpu10I CC'TnA_GC
Cuts at: 0 385 954
Size: 385 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 954
Size: 441 513
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 954
Size: 183 771
Bsp1286I G_dGCh'C
Cuts at: 0 115 954
Size: 115 839
BssHII G'CGCG_C
Cuts at: 0 88 90 92 954
Size: 88 2 2 862
Fragments arranged by size:
862 88 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 954
Size: 54 900
BstYI r'GATC_y
Cuts at: 0 240 668 954
Size: 240 428 286
Fragments arranged by size:
428 286 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 8 404 954
Size: 8 396 550
Fragments arranged by size:
550 396 8
BtsI GCAGTG_nn'
Cuts at: 0 166 954
Size: 166 788
DraI TTT'AAA
Cuts at: 0 222 954
Size: 222 732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 625 769 954
Size: 19 606 144 185
Fragments arranged by size:
606 185 144 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 643 954
Size: 643 311
EcoO109I rG'GnC_Cy
Cuts at: 0 694 954
Size: 694 260
HaeII r_GCGC'y
Cuts at: 0 232 954
Size: 232 722
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 414 446 448 480 954
Size: 232 32 150 32 2 32 474
Fragments arranged by size:
474 232 150 32 32 32 2
HincII GTy'rAC
Cuts at: 0 348 433 954
Size: 348 85 521
Fragments arranged by size:
521 348 85
HpaI GTT'AAC
Cuts at: 0 348 954
Size: 348 606
MspA1I CmG'CkG
Cuts at: 0 39 149 186 773 954
Size: 39 110 37 587 181
Fragments arranged by size:
587 181 110 39 37
NsiI A_TGCA'T
Cuts at: 0 683 954
Size: 683 271
PasI CC'CwG_GG
Cuts at: 0 9 954
Size: 9 945
PfoI T'CCnGG_A
Cuts at: 0 734 940 954
Size: 734 206 14
Fragments arranged by size:
734 206 14
PpuMI rG'GwC_Cy
Cuts at: 0 694 954
Size: 694 260
PstI C_TGCA'G
Cuts at: 0 50 954
Size: 50 904
PvuII CAG'CTG
Cuts at: 0 39 773 954
Size: 39 734 181
Fragments arranged by size:
734 181 39
SalI G'TCGA_C
Cuts at: 0 431 954
Size: 431 523
SanDI GG'GwC_CC
Cuts at: 0 694 954
Size: 694 260
SfcI C'TryA_G
Cuts at: 0 46 954
Size: 46 908
SmlI C'TyrA_G
Cuts at: 0 420 954
Size: 420 534
StyI C'CwwG_G
Cuts at: 0 539 954
Size: 539 415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 954
Size: 841 113
TatI w'GTAC_w
Cuts at: 0 767 954
Size: 767 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 686 718 954
Size: 686 32 236
Fragments arranged by size:
686 236 32
Enzymes that do cut:
AccI AcuI AloI AlwNI ApoI AseI AvaI BamHI
BanI BbsI BfrBI BglII Bme1580I BmgBI BpmI Bpu10I
BpuEI BsgI Bsp1286I BssHII BstAPI BstYI BtgZI BtsI
DraI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI
MspA1I NsiI PasI PfoI PpuMI PstI PvuII SalI
SanDI SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII
BaeI BanII BbeI BbvCI BcgI BciVI BclI BglI
BlpI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI