(Linear) (Six-base) MAPSORT of: KIEE1802.seq  Check: 5069  from: 1  to: 939



KIEE1802.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AclI AA'CG_TT

Cuts at:      0     594     939 
   Size:        594     345
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     866     939 
   Size:        121     745      73

  Fragments arranged by size: 

                 745     121      73
 
ApoI r'AATT_y

Cuts at:      0     334     363     428     939 
   Size:        334      29      65     511

  Fragments arranged by size: 

                 511     334      65      29
 
AvaI C'yCGr_G

Cuts at:      0     653     939 
   Size:        653     286
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     719     939 
   Size:        719     220
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     727     939 
   Size:        727     212
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     477     939 
   Size:        477     462
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812     939 
   Size:        812     127
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     562     939 
   Size:        562     377
 
BsrDI GCAATG_nn'

Cuts at:      0     560     939 
   Size:        560     379
 
BtgI C'CryG_G

Cuts at:      0     436     939 
   Size:        436     503
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     552     939 
   Size:        552     387
 
DraI TTT'AAA

Cuts at:      0     225     939 
   Size:        225     714
 
DraIII CAC_nnn'GTG

Cuts at:      0     691     939 
   Size:        691     248
 
EaeI y'GGCC_r

Cuts at:      0     557     939 
   Size:        557     382
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     477     866     939 
   Size:        121     356     389      73

  Fragments arranged by size: 

                 389     356     121      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     658     939 
   Size:        181     477     281

  Fragments arranged by size: 

                 477     281     181
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     850     882     939 
   Size:         26     824      32      57

  Fragments arranged by size: 

                 824      57      32      26
 
HindIII A'AGCT_T

Cuts at:      0     830     939 
   Size:        830     109
 
MscI TGG'CCA

Cuts at:      0     559     939 
   Size:        559     380
 
MslI CAynn'nnrTG

Cuts at:      0     608     939 
   Size:        608     331
 
MspA1I CmG'CkG

Cuts at:      0     648     939 
   Size:        648     291
 
NcoI C'CATG_G

Cuts at:      0     436     939 
   Size:        436     503
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     626     658     939 
   Size:        626      32     281

  Fragments arranged by size: 

                 626     281      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     181     658     939 
   Size:        181     477     281

  Fragments arranged by size: 

                 477     281     181
 
PvuII CAG'CTG

Cuts at:      0     648     939 
   Size:        648     291
 
SfcI C'TryA_G

Cuts at:      0     516     939 
   Size:        516     423
 
SmaI CCC'GGG

Cuts at:      0     655     939 
   Size:        655     284
 
SmlI C'TyrA_G

Cuts at:      0     827     939 
   Size:        827     112
 
StyI C'CwwG_G

Cuts at:      0     436     939 
   Size:        436     503
 
Tth111I GACn'n_nGTC

Cuts at:      0     185     939 
   Size:        185     754
 
XmaI C'CCGG_G

Cuts at:      0     653     939 
   Size:        653     286
 
XmnI GAAnn'nnTTC

Cuts at:      0     431     939 
   Size:        431     508
 

 Enzymes that do cut:

     AclI     AcuI     ApoI     AvaI     BbsI     BmrI     BpmI    BpuEI
    BseRI    BsrDI     BtgI    BtgZI     DraI   DraIII     EaeI  Eco57MI
 EcoO109I    Hin4I  HindIII     MscI     MslI   MspA1I     NcoI     PpiI
    PpuMI    PvuII     SfcI     SmaI     SmlI     StyI  Tth111I     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     ZraI