Restriction map for
pFN21AE1801
(Linear) (Six-base) MAPSORT of: KIEE1801.seq Check: 103 from: 1 to: 939
KIEE1801.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 939
Size: 650 289
AflIII A'CryG_T
Cuts at: 0 382 738 939
Size: 382 356 201
Fragments arranged by size:
382 356 201
ApaLI G'TGCA_C
Cuts at: 0 3 302 939
Size: 3 299 637
Fragments arranged by size:
637 299 3
ApoI r'AATT_y
Cuts at: 0 229 342 623 939
Size: 229 113 281 316
Fragments arranged by size:
316 281 229 113
AvaI C'yCGr_G
Cuts at: 0 309 939
Size: 309 630
BanI G'GyrC_C
Cuts at: 0 190 356 939
Size: 190 166 583
Fragments arranged by size:
583 190 166
BanII G_rGCy'C
Cuts at: 0 19 316 939
Size: 19 297 623
Fragments arranged by size:
623 297 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 566 939
Size: 566 373
BfrBI ATG'CAT
Cuts at: 0 54 939
Size: 54 885
BglII A'GATC_T
Cuts at: 0 114 818 939
Size: 114 704 121
Fragments arranged by size:
704 121 114
Bme1580I G_kGCm'C
Cuts at: 0 7 306 939
Size: 7 299 633
Fragments arranged by size:
633 299 7
BmtI G_CTAG'C
Cuts at: 0 76 939
Size: 76 863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 693 939
Size: 693 246
BsaI GGTCTCn'nnnn_
Cuts at: 0 909 939
Size: 909 30
BsaBI GATnn'nnATC
Cuts at: 0 53 939
Size: 53 886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 773 939
Size: 773 166
BseYI C'CCAG_C
Cuts at: 0 764 939
Size: 764 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 939
Size: 712 227
BsiHKAI G_wGCw'C
Cuts at: 0 7 306 939
Size: 7 299 633
Fragments arranged by size:
633 299 7
BsmI GAATG_Cn'
Cuts at: 0 815 939
Size: 815 124
Bsp1286I G_dGCh'C
Cuts at: 0 7 19 306 316 939
Size: 7 12 287 10 623
Fragments arranged by size:
623 287 12 10 7
BsrBI CCG'CTC
Cuts at: 0 672 939
Size: 672 267
BstYI r'GATC_y
Cuts at: 0 114 284 633 707 818 939
Size: 114 170 349 74 111 121
Fragments arranged by size:
349 170 121 114 111 74
BtsI GCAGTG_nn'
Cuts at: 0 5 36 620 939
Size: 5 31 584 319
Fragments arranged by size:
584 319 31 5
EaeI y'GGCC_r
Cuts at: 0 456 939
Size: 456 483
EarI CTCTTCn'nnn_
Cuts at: 0 718 939
Size: 718 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 939
Size: 650 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 550 729 939
Size: 550 179 210
Fragments arranged by size:
550 210 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 692 724 939
Size: 692 32 215
Fragments arranged by size:
692 215 32
HincII GTy'rAC
Cuts at: 0 131 205 939
Size: 131 74 734
Fragments arranged by size:
734 131 74
HpaI GTT'AAC
Cuts at: 0 131 939
Size: 131 808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 889 939
Size: 161 728 50
Fragments arranged by size:
728 161 50
MslI CAynn'nnrTG
Cuts at: 0 721 939
Size: 721 218
NheI G'CTAG_C
Cuts at: 0 72 939
Size: 72 867
NsiI A_TGCA'T
Cuts at: 0 56 939
Size: 56 883
NspI r_CATG'y
Cuts at: 0 742 939
Size: 742 197
PciI A'CATG_T
Cuts at: 0 738 939
Size: 738 201
PsiI TTA'TAA
Cuts at: 0 917 939
Size: 917 22
PstI C_TGCA'G
Cuts at: 0 818 939
Size: 818 121
SfcI C'TryA_G
Cuts at: 0 814 939
Size: 814 125
StyI C'CwwG_G
Cuts at: 0 681 939
Size: 681 258
TatI w'GTAC_w
Cuts at: 0 589 939
Size: 589 350
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 939
Size: 367 32 540
Fragments arranged by size:
540 367 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 477 939
Size: 477 462
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AvaI BanI BanII BbsI
BfrBI BglII Bme1580I BmtI BplI BsaI BsaBI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BstYI BtsI
EaeI EarI Eco57MI FalI Hin4I HincII HpaI MmeI
MslI NheI NsiI NspI PciI PsiI PstI SfcI
StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglI BlpI BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI