Restriction map for
pFN21AE1796
(Linear) (Six-base) MAPSORT of: KIEE1796.seq Check: 6667 from: 1 to: 939
KIEE1796.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 578 939
Size: 578 361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 337 939
Size: 337 602
ApaI G_GGCC'C
Cuts at: 0 174 182 263 939
Size: 174 8 81 676
Fragments arranged by size:
676 174 81 8
BanI G'GyrC_C
Cuts at: 0 196 939
Size: 196 743
BanII G_rGCy'C
Cuts at: 0 75 127 174 182 263 284 572 939
Size: 75 52 47 8 81 21 288 367
Fragments arranged by size:
367 288 81 75 52 47 21 8
BciVI GTATCCnnnnn_n'
Cuts at: 0 886 939
Size: 886 53
BglI GCCn_nnn'nGGC
Cuts at: 0 122 299 643 939
Size: 122 177 344 296
Fragments arranged by size:
344 296 177 122
Bme1580I G_kGCm'C
Cuts at: 0 174 182 263 338 939
Size: 174 8 81 75 601
Fragments arranged by size:
601 174 81 75 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 483 939
Size: 483 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 139 939
Size: 61 78 800
Fragments arranged by size:
800 78 61
Bpu10I CC'TnA_GC
Cuts at: 0 343 939
Size: 343 596
BsaAI yAC'GTr
Cuts at: 0 482 939
Size: 482 457
BsaWI w'CCGG_w
Cuts at: 0 760 939
Size: 760 179
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 227 939
Size: 227 712
BseYI C'CCAG_C
Cuts at: 0 284 507 522 939
Size: 284 223 15 417
Fragments arranged by size:
417 284 223 15
BsiHKAI G_wGCw'C
Cuts at: 0 273 395 572 939
Size: 273 122 177 367
Fragments arranged by size:
367 273 177 122
Bsp1286I G_dGCh'C
Cuts at: 0 75 127 174 182 263 273 284 338
Size: 75 52 47 8 81 10 11 54
Cuts at: 338 395 572 939
Size: 57 177 367
Fragments arranged by size:
367 177 81 75 57 54 52 47
11 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 319 329 374 939
Size: 319 10 45 565
Fragments arranged by size:
565 319 45 10
BsrBI CCG'CTC
Cuts at: 0 347 939
Size: 347 592
BsrFI r'CCGG_y
Cuts at: 0 377 939
Size: 377 562
Bsu36I CC'TnA_GG
Cuts at: 0 838 939
Size: 838 101
BtsI GCAGTG_nn'
Cuts at: 0 693 939
Size: 693 246
EaeI y'GGCC_r
Cuts at: 0 635 939
Size: 635 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 661 939
Size: 661 278
EcoICRI GAG'CTC
Cuts at: 0 570 939
Size: 570 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 139 939
Size: 61 78 800
Fragments arranged by size:
800 78 61
EcoO109I rG'GnC_Cy
Cuts at: 0 24 170 171 318 671 939
Size: 24 146 1 147 353 268
Fragments arranged by size:
353 268 147 146 24 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 510 939
Size: 510 429
HaeII r_GCGC'y
Cuts at: 0 35 939
Size: 35 904
MspA1I CmG'CkG
Cuts at: 0 438 939
Size: 438 501
NaeI GCC'GGC
Cuts at: 0 379 939
Size: 379 560
NgoMIV G'CCGG_C
Cuts at: 0 377 939
Size: 377 562
PasI CC'CwG_GG
Cuts at: 0 175 555 939
Size: 175 380 384
Fragments arranged by size:
384 380 175
PpuMI rG'GwC_Cy
Cuts at: 0 24 318 939
Size: 24 294 621
Fragments arranged by size:
621 294 24
PshAI GACnn'nnGTC
Cuts at: 0 92 939
Size: 92 847
PspOMI G'GGCC_C
Cuts at: 0 170 178 259 939
Size: 170 8 81 680
Fragments arranged by size:
680 170 81 8
SacI G_AGCT'C
Cuts at: 0 572 939
Size: 572 367
SexAI A'CCwGG_T
Cuts at: 0 581 939
Size: 581 358
StyI C'CwwG_G
Cuts at: 0 182 939
Size: 182 757
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 344 939
Size: 344 595
TatI w'GTAC_w
Cuts at: 0 617 653 939
Size: 617 36 286
Fragments arranged by size:
617 286 36
Enzymes that do cut:
AccI AlfI ApaI BanI BanII BciVI BglI Bme1580I
BmrI BpmI Bpu10I BsaAI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI Bsu36I BtsI EaeI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII MspA1I NaeI NgoMIV
PasI PpuMI PshAI PspOMI SacI SexAI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI