Restriction map for
pFN21AE1793
(Linear) (Six-base) MAPSORT of: KIEE1793.seq Check: 5844 from: 1 to: 912
KIEE1793.orf
With 182 enzymes: SgfI *
July 3, 2012 17:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 912
Size: 683 229
ApaI G_GGCC'C
Cuts at: 0 535 912
Size: 535 377
ApoI r'AATT_y
Cuts at: 0 244 912
Size: 244 668
AvaI C'yCGr_G
Cuts at: 0 405 912
Size: 405 507
BanI G'GyrC_C
Cuts at: 0 694 912
Size: 694 218
BanII G_rGCy'C
Cuts at: 0 104 314 326 535 912
Size: 104 210 12 209 377
Fragments arranged by size:
377 210 209 104 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 671 830 912
Size: 671 159 82
Fragments arranged by size:
671 159 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 493 912
Size: 493 419
BlpI GC'TnA_GC
Cuts at: 0 832 912
Size: 832 80
Bme1580I G_kGCm'C
Cuts at: 0 535 697 912
Size: 535 162 215
Fragments arranged by size:
535 215 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 599 912
Size: 599 313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 912
Size: 709 203
Bpu10I CC'TnA_GC
Cuts at: 0 224 912
Size: 224 688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 912
Size: 451 461
BsaAI yAC'GTr
Cuts at: 0 489 912
Size: 489 423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 301 331 401 431 912
Size: 250 30 21 30 70 30 481
Fragments arranged by size:
481 250 70 30 30 30 21
BseYI C'CCAG_C
Cuts at: 0 62 325 912
Size: 62 263 587
Fragments arranged by size:
587 263 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 912
Size: 163 749
BsiHKAI G_wGCw'C
Cuts at: 0 314 912
Size: 314 598
BsmBI CGTCTCn'nnnn_
Cuts at: 0 709 912
Size: 709 203
Bsp1286I G_dGCh'C
Cuts at: 0 104 314 326 535 697 912
Size: 104 210 12 209 162 215
Fragments arranged by size:
215 210 209 162 104 12
BsrBI CCG'CTC
Cuts at: 0 228 912
Size: 228 684
BstEII G'GTnAC_C
Cuts at: 0 417 912
Size: 417 495
BstYI r'GATC_y
Cuts at: 0 339 478 912
Size: 339 139 434
Fragments arranged by size:
434 339 139
BtgI C'CryG_G
Cuts at: 0 203 912
Size: 203 709
BtsI GCAGTG_nn'
Cuts at: 0 440 752 912
Size: 440 312 160
Fragments arranged by size:
440 312 160
EarI CTCTTCn'nnn_
Cuts at: 0 835 912
Size: 835 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 912
Size: 532 380
EcoICRI GAG'CTC
Cuts at: 0 312 912
Size: 312 600
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 709 912
Size: 683 26 203
Fragments arranged by size:
683 203 26
EcoO109I rG'GnC_Cy
Cuts at: 0 532 912
Size: 532 380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 211 438 912
Size: 211 227 474
Fragments arranged by size:
474 227 211
HaeII r_GCGC'y
Cuts at: 0 62 912
Size: 62 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 301 333 463 495 912
Size: 250 32 19 32 130 32 417
Fragments arranged by size:
417 250 130 32 32 32 19
MslI CAynn'nnrTG
Cuts at: 0 564 912
Size: 564 348
MspA1I CmG'CkG
Cuts at: 0 177 290 912
Size: 177 113 622
Fragments arranged by size:
622 177 113
NspI r_CATG'y
Cuts at: 0 456 912
Size: 456 456
PasI CC'CwG_GG
Cuts at: 0 528 912
Size: 528 384
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 912
Size: 111 801
PfoI T'CCnGG_A
Cuts at: 0 254 387 912
Size: 254 133 525
Fragments arranged by size:
525 254 133
PspOMI G'GGCC_C
Cuts at: 0 531 912
Size: 531 381
PstI C_TGCA'G
Cuts at: 0 684 912
Size: 684 228
PvuII CAG'CTG
Cuts at: 0 177 290 912
Size: 177 113 622
Fragments arranged by size:
622 177 113
SacI G_AGCT'C
Cuts at: 0 314 912
Size: 314 598
SfcI C'TryA_G
Cuts at: 0 680 912
Size: 680 232
SmlI C'TyrA_G
Cuts at: 0 466 912
Size: 466 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 384 912
Size: 384 528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 401 415 433 912
Size: 383 18 14 18 479
Fragments arranged by size:
479 383 18 18 14
XbaI T'CTAG_A
Cuts at: 0 435 912
Size: 435 477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 333 912
Size: 154 179 579
Fragments arranged by size:
579 179 154
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanI BanII BbsI BciVI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BstEII BstYI
BtgI BtsI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I MslI MspA1I NspI PasI PflMI PfoI
PspOMI PstI PvuII SacI SfcI SmlI TaqII TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BmgBI BmtI BplI BsaI
BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XhoI XmaI
XmnI ZraI