Restriction map for
pFN21AE1789
(Linear) (Six-base) MAPSORT of: KIEE1789.seq Check: 4448 from: 1 to: 888
KIEE1789.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 827 888
Size: 827 61
AleI CACnn'nnGTG
Cuts at: 0 363 888
Size: 363 525
ApoI r'AATT_y
Cuts at: 0 550 752 888
Size: 550 202 136
Fragments arranged by size:
550 202 136
AvaI C'yCGr_G
Cuts at: 0 157 888
Size: 157 731
BanII G_rGCy'C
Cuts at: 0 58 392 877 888
Size: 58 334 485 11
Fragments arranged by size:
485 334 58 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 801 888
Size: 801 87
BbvCI CC'TCA_GC
Cuts at: 0 226 888
Size: 226 662
BciVI GTATCCnnnnn_n'
Cuts at: 0 777 888
Size: 777 111
BglII A'GATC_T
Cuts at: 0 816 888
Size: 816 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 337 515 888
Size: 337 178 373
Fragments arranged by size:
373 337 178
Bpu10I CC'TnA_GC
Cuts at: 0 57 226 493 888
Size: 57 169 267 395
Fragments arranged by size:
395 267 169 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 888
Size: 406 482
BsaWI w'CCGG_w
Cuts at: 0 134 888
Size: 134 754
BseYI C'CCAG_C
Cuts at: 0 48 74 258 888
Size: 48 26 184 630
Fragments arranged by size:
630 184 48 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 719 888
Size: 387 332 169
Fragments arranged by size:
387 332 169
BsiHKAI G_wGCw'C
Cuts at: 0 392 591 888
Size: 392 199 297
Fragments arranged by size:
392 297 199
Bsp1286I G_dGCh'C
Cuts at: 0 58 392 591 877 888
Size: 58 334 199 286 11
Fragments arranged by size:
334 286 199 58 11
BspHI T'CATG_A
Cuts at: 0 110 852 888
Size: 110 742 36
Fragments arranged by size:
742 110 36
BsrBI CCG'CTC
Cuts at: 0 164 888
Size: 164 724
BsrDI GCAATG_nn'
Cuts at: 0 491 888
Size: 491 397
BstBI TT'CG_AA
Cuts at: 0 476 564 888
Size: 476 88 324
Fragments arranged by size:
476 324 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 298 888
Size: 298 590
BstYI r'GATC_y
Cuts at: 0 816 888
Size: 816 72
BtgI C'CryG_G
Cuts at: 0 447 888
Size: 447 441
ClaI AT'CG_AT
Cuts at: 0 774 888
Size: 774 114
EaeI y'GGCC_r
Cuts at: 0 11 305 888
Size: 11 294 583
Fragments arranged by size:
583 294 11
EarI CTCTTCn'nnn_
Cuts at: 0 596 888
Size: 596 292
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 888
Size: 100 788
EcoICRI GAG'CTC
Cuts at: 0 390 888
Size: 390 498
EcoO109I rG'GnC_Cy
Cuts at: 0 45 320 411 888
Size: 45 275 91 477
Fragments arranged by size:
477 275 91 45
EcoRI G'AATT_C
Cuts at: 0 752 888
Size: 752 136
HincII GTy'rAC
Cuts at: 0 381 888
Size: 381 507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 888
Size: 76 812
MslI CAynn'nnrTG
Cuts at: 0 363 888
Size: 363 525
MspA1I CmG'CkG
Cuts at: 0 52 399 421 504 733 888
Size: 52 347 22 83 229 155
Fragments arranged by size:
347 229 155 83 52 22
NcoI C'CATG_G
Cuts at: 0 447 888
Size: 447 441
PpuMI rG'GwC_Cy
Cuts at: 0 45 320 411 888
Size: 45 275 91 477
Fragments arranged by size:
477 275 91 45
PvuII CAG'CTG
Cuts at: 0 504 733 888
Size: 504 229 155
Fragments arranged by size:
504 229 155
SacI G_AGCT'C
Cuts at: 0 392 888
Size: 392 496
SanDI GG'GwC_CC
Cuts at: 0 45 320 888
Size: 45 275 568
Fragments arranged by size:
568 275 45
SapI GCTCTTCn'nnn_
Cuts at: 0 596 888
Size: 596 292
ScaI AGT'ACT
Cuts at: 0 725 888
Size: 725 163
SfcI C'TryA_G
Cuts at: 0 789 888
Size: 789 99
SmaI CCC'GGG
Cuts at: 0 159 888
Size: 159 729
SmlI C'TyrA_G
Cuts at: 0 385 888
Size: 385 503
SspI AAT'ATT
Cuts at: 0 569 688 888
Size: 569 119 200
Fragments arranged by size:
569 200 119
StyI C'CwwG_G
Cuts at: 0 447 888
Size: 447 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 417 888
Size: 250 167 471
Fragments arranged by size:
471 250 167
TatI w'GTAC_w
Cuts at: 0 274 723 888
Size: 274 449 165
Fragments arranged by size:
449 274 165
Tth111I GACn'n_nGTC
Cuts at: 0 427 888
Size: 427 461
XmaI C'CCGG_G
Cuts at: 0 157 888
Size: 157 731
Enzymes that do cut:
AccI AleI ApoI AvaI BanII BbsI BbvCI BciVI
BglII BmrI Bpu10I BpuEI BsaWI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BstBI BstXI BstYI BtgI
ClaI EaeI EarI EciI EcoICRI EcoO109I EcoRI HincII
MmeI MslI MspA1I NcoI PpuMI PvuII SacI SanDI
SapI ScaI SfcI SmaI SmlI SspI StyI TaqII
TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EagI Eco57MI EcoNI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI TstI XbaI XcmI XhoI XmnI
ZraI