(Linear) (Six-base) MAPSORT of: KIEE1786.seq  Check: 1390  from: 1  to: 882



KIEE1786.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

ApoI r'AATT_y

Cuts at:      0     637     882 
   Size:        637     245
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     792     825     882 
   Size:        792      33      57

  Fragments arranged by size: 

                 792      57      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     317     882 
   Size:        317     565
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     622     882 
   Size:        622     260
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501     882 
   Size:        501     381
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     882 
   Size:        452     430
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     527     882 
   Size:        527     355
 
BsaHI Gr'CG_yC

Cuts at:      0      32     882 
   Size:         32     850
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     882 
   Size:        415     467
 
BseYI C'CCAG_C

Cuts at:      0     327     882 
   Size:        327     555
 
BsiHKAI G_wGCw'C

Cuts at:      0     868     882 
   Size:        868      14
 
BsmI GAATG_Cn'

Cuts at:      0     468     882 
   Size:        468     414
 
Bsp1286I G_dGCh'C

Cuts at:      0     868     882 
   Size:        868      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     228     882 
   Size:        228     654
 
BsrDI GCAATG_nn'

Cuts at:      0     335     882 
   Size:        335     547
 
BtgI C'CryG_G

Cuts at:      0     565     882 
   Size:        565     317
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      19      98     882 
   Size:         19      79     784

  Fragments arranged by size: 

                 784      79      19
 
BtsI GCAGTG_nn'

Cuts at:      0      27     882 
   Size:         27     855
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501     882 
   Size:        501     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     798     830     882 
   Size:        798      32      52

  Fragments arranged by size: 

                 798      52      32
 
MfeI C'AATT_G

Cuts at:      0     344     882 
   Size:        344     538
 
MspA1I CmG'CkG

Cuts at:      0      14     882 
   Size:         14     868
 
NcoI C'CATG_G

Cuts at:      0     565     882 
   Size:        565     317
 
NdeI CA'TA_TG

Cuts at:      0     126     745     882 
   Size:        126     619     137

  Fragments arranged by size: 

                 619     137     126
 
NspI r_CATG'y

Cuts at:      0     157     882 
   Size:        157     725
 
PfoI T'CCnGG_A

Cuts at:      0      35     525     882 
   Size:         35     490     357

  Fragments arranged by size: 

                 490     357      35
 
PstI C_TGCA'G

Cuts at:      0     258     882 
   Size:        258     624
 
SfcI C'TryA_G

Cuts at:      0     254     882 
   Size:        254     628
 
SmlI C'TyrA_G

Cuts at:      0     467     882 
   Size:        467     415
 
SphI G_CATG'C

Cuts at:      0     157     882 
   Size:        157     725
 
StyI C'CwwG_G

Cuts at:      0     565     882 
   Size:        565     317
 

 Enzymes that do cut:

     ApoI     BaeI     BbsI     BmrI     BpmI    BpuEI     BsaI    BsaHI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI     BtgI
    BtgZI     BtsI  Eco57MI    Hin4I     MfeI   MspA1I     NcoI     NdeI
     NspI     PfoI     PstI     SfcI     SmlI     SphI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI