Restriction map for
pFN21AE1783
(Linear) (Six-base) MAPSORT of: KIEE1783.seq Check: 1100 from: 1 to: 867
KIEE1783.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 398 624 867
Size: 398 226 243
Fragments arranged by size:
398 243 226
AccI GT'mk_AC
Cuts at: 0 710 867
Size: 710 157
AflIII A'CryG_T
Cuts at: 0 488 867
Size: 488 379
AlwNI CAG_nnn'CTG
Cuts at: 0 657 867
Size: 657 210
AvaI C'yCGr_G
Cuts at: 0 217 431 867
Size: 217 214 436
Fragments arranged by size:
436 217 214
BanI G'GyrC_C
Cuts at: 0 331 867
Size: 331 536
BanII G_rGCy'C
Cuts at: 0 224 867
Size: 224 643
BbsI GAAGACnn'nnnn_
Cuts at: 0 525 867
Size: 525 342
BclI T'GATC_A
Cuts at: 0 26 867
Size: 26 841
BglI GCCn_nnn'nGGC
Cuts at: 0 161 188 500 773 833 867
Size: 161 27 312 273 60 34
Fragments arranged by size:
312 273 161 60 34 27
BlpI GC'TnA_GC
Cuts at: 0 682 867
Size: 682 185
Bme1580I G_kGCm'C
Cuts at: 0 102 754 867
Size: 102 652 113
Fragments arranged by size:
652 113 102
BmgBI CAC'GTC
Cuts at: 0 176 489 867
Size: 176 313 378
Fragments arranged by size:
378 313 176
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 867
Size: 67 800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 867
Size: 712 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 867
Size: 571 296
BsaAI yAC'GTr
Cuts at: 0 519 867
Size: 519 348
BsaHI Gr'CG_yC
Cuts at: 0 527 581 867
Size: 527 54 286
Fragments arranged by size:
527 286 54
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 451 867
Size: 34 417 416
Fragments arranged by size:
417 416 34
BseYI C'CCAG_C
Cuts at: 0 191 464 867
Size: 191 273 403
Fragments arranged by size:
403 273 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 190 376 867
Size: 109 81 186 491
Fragments arranged by size:
491 186 109 81
BsiEI CG_ry'CG
Cuts at: 0 153 183 867
Size: 153 30 684
Fragments arranged by size:
684 153 30
BsiHKAI G_wGCw'C
Cuts at: 0 224 452 688 867
Size: 224 228 236 179
Fragments arranged by size:
236 228 224 179
Bsp1286I G_dGCh'C
Cuts at: 0 102 224 452 688 754 867
Size: 102 122 228 236 66 113
Fragments arranged by size:
236 228 122 113 102 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 398 624 867
Size: 245 153 226 243
Fragments arranged by size:
245 243 226 153
BsrDI GCAATG_nn'
Cuts at: 0 77 371 867
Size: 77 294 496
Fragments arranged by size:
496 294 77
BsrFI r'CCGG_y
Cuts at: 0 334 772 832 867
Size: 334 438 60 35
Fragments arranged by size:
438 334 60 35
BssHII G'CGCG_C
Cuts at: 0 664 867
Size: 664 203
BstEII G'GTnAC_C
Cuts at: 0 385 867
Size: 385 482
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 554 867
Size: 294 260 313
Fragments arranged by size:
313 294 260
BstYI r'GATC_y
Cuts at: 0 254 867
Size: 254 613
BtgI C'CryG_G
Cuts at: 0 63 380 563 821 867
Size: 63 317 183 258 46
Fragments arranged by size:
317 258 183 63 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 867
Size: 258 609
BtsI GCAGTG_nn'
Cuts at: 0 86 345 504 867
Size: 86 259 159 363
Fragments arranged by size:
363 259 159 86
DraIII CAC_nnn'GTG
Cuts at: 0 399 456 867
Size: 399 57 411
Fragments arranged by size:
411 399 57
EaeI y'GGCC_r
Cuts at: 0 44 150 180 521 867
Size: 44 106 30 341 346
Fragments arranged by size:
346 341 106 44 30
EagI C'GGCC_G
Cuts at: 0 150 180 867
Size: 150 30 687
Fragments arranged by size:
687 150 30
EcoICRI GAG'CTC
Cuts at: 0 222 867
Size: 222 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 867
Size: 712 155
FspI TGC'GCA
Cuts at: 0 478 721 867
Size: 478 243 146
Fragments arranged by size:
478 243 146
FspAI rTGC'GCAy
Cuts at: 0 478 721 867
Size: 478 243 146
Fragments arranged by size:
478 243 146
HaeII r_GCGC'y
Cuts at: 0 606 658 867
Size: 606 52 209
Fragments arranged by size:
606 209 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 867
Size: 532 32 303
Fragments arranged by size:
532 303 32
HincII GTy'rAC
Cuts at: 0 141 867
Size: 141 726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 803 867
Size: 275 528 64
Fragments arranged by size:
528 275 64
MslI CAynn'nnrTG
Cuts at: 0 286 289 808 867
Size: 286 3 519 59
Fragments arranged by size:
519 286 59 3
MspA1I CmG'CkG
Cuts at: 0 417 464 800 867
Size: 417 47 336 67
Fragments arranged by size:
417 336 67 47
NaeI GCC'GGC
Cuts at: 0 774 867
Size: 774 93
NcoI C'CATG_G
Cuts at: 0 63 867
Size: 63 804
NgoMIV G'CCGG_C
Cuts at: 0 772 867
Size: 772 95
NspI r_CATG'y
Cuts at: 0 650 867
Size: 650 217
PasI CC'CwG_GG
Cuts at: 0 554 867
Size: 554 313
PflMI CCAn_nnn'nTGG
Cuts at: 0 657 867
Size: 657 210
PfoI T'CCnGG_A
Cuts at: 0 443 536 867
Size: 443 93 331
Fragments arranged by size:
443 331 93
PmlI CAC'GTG
Cuts at: 0 519 867
Size: 519 348
SacI G_AGCT'C
Cuts at: 0 224 867
Size: 224 643
SmaI CCC'GGG
Cuts at: 0 433 867
Size: 433 434
SmlI C'TyrA_G
Cuts at: 0 586 867
Size: 586 281
StyI C'CwwG_G
Cuts at: 0 63 167 867
Size: 63 104 700
Fragments arranged by size:
700 104 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 393 456 496 867
Size: 183 210 63 40 371
Fragments arranged by size:
371 210 183 63 40
Tth111I GACn'n_nGTC
Cuts at: 0 529 867
Size: 529 338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 115 867
Size: 54 61 752
Fragments arranged by size:
752 61 54
XmaI C'CCGG_G
Cuts at: 0 431 867
Size: 431 436
Enzymes that do cut:
AarI AccI AflIII AlwNI AvaI BanI BanII BbsI
BclI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BssHII BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EcoICRI Eco57MI FspI
FspAI HaeII Hin4I HincII MmeI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI SacI
SmaI SmlI StyI TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglII BmtI BplI Bpu10I BsaI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EarI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI HindIII HpaI KasI KpnI MfeI MluI MscI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI XhoI XmnI ZraI