(Linear) (Six-base) MAPSORT of: KIEE1782.seq  Check: 7914  from: 1  to: 723



KIEE1782.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     723 
   Size:        596     127
 
AflII C'TTAA_G

Cuts at:      0     463     723 
   Size:        463     260
 
AflIII A'CryG_T

Cuts at:      0     232     723 
   Size:        232     491
 
AhdI GACnn_n'nnGTC

Cuts at:      0     330     723 
   Size:        330     393
 
AlwNI CAG_nnn'CTG

Cuts at:      0     523     723 
   Size:        523     200
 
ApoI r'AATT_y

Cuts at:      0     351     498     527     723 
   Size:        351     147      29     196

  Fragments arranged by size: 

                 351     196     147      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10      39     723 
   Size:         10      29     684

  Fragments arranged by size: 

                 684      29      10
 
BfrBI ATG'CAT

Cuts at:      0     703     723 
   Size:        703      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      38     723 
   Size:         38     685
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     645     723 
   Size:        645      78
 
BsmI GAATG_Cn'

Cuts at:      0     175     723 
   Size:        175     548
 
BsrDI GCAATG_nn'

Cuts at:      0       7     723 
   Size:          7     716
 
BsrGI T'GTAC_A

Cuts at:      0     105     723 
   Size:        105     618
 
BstYI r'GATC_y

Cuts at:      0     153     723 
   Size:        153     570
 
BtgI C'CryG_G

Cuts at:      0      84     723 
   Size:         84     639
 
EarI CTCTTCn'nnn_

Cuts at:      0     266     723 
   Size:        266     457
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     723 
   Size:        596     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     723 
   Size:        316      32     375

  Fragments arranged by size: 

                 375     316      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     723 
   Size:        244     479
 
MslI CAynn'nnrTG

Cuts at:      0     708     712     723 
   Size:        708       4      11

  Fragments arranged by size: 

                 708      11       4
 
MspA1I CmG'CkG

Cuts at:      0     341     723 
   Size:        341     382
 
NcoI C'CATG_G

Cuts at:      0      84     723 
   Size:         84     639
 
NdeI CA'TA_TG

Cuts at:      0     709     723 
   Size:        709      14
 
NsiI A_TGCA'T

Cuts at:      0     705     723 
   Size:        705      18
 
NspI r_CATG'y

Cuts at:      0     236     723 
   Size:        236     487
 
PciI A'CATG_T

Cuts at:      0     232     723 
   Size:        232     491
 
ScaI AGT'ACT

Cuts at:      0     509     723 
   Size:        509     214
 
SexAI A'CCwGG_T

Cuts at:      0     446     723 
   Size:        446     277
 
SmlI C'TyrA_G

Cuts at:      0     463     660     723 
   Size:        463     197      63

  Fragments arranged by size: 

                 463     197      63
 
StuI AGG'CCT

Cuts at:      0     198     723 
   Size:        198     525
 
StyI C'CwwG_G

Cuts at:      0      84     723 
   Size:         84     639
 
TatI w'GTAC_w

Cuts at:      0     105     507     723 
   Size:        105     402     216

  Fragments arranged by size: 

                 402     216     105
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     646     723 
   Size:        646      77
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AhdI    AlwNI     ApoI     BbsI    BfrBI
     BmrI    BpuEI     BsmI    BsrDI    BsrGI    BstYI     BtgI     EarI
  Eco57MI    Hin4I     MmeI     MslI   MspA1I     NcoI     NdeI     NsiI
     NspI     PciI     ScaI    SexAI     SmlI     StuI     StyI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI