Restriction map for
pFN21AE1781
(Linear) (Six-base) MAPSORT of: KIEE1781.seq Check: 6861 from: 1 to: 867
KIEE1781.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
Acc65I G'GTAC_C
Cuts at: 0 269 867
Size: 269 598
AflIII A'CryG_T
Cuts at: 0 257 867
Size: 257 610
AhdI GACnn_n'nnGTC
Cuts at: 0 700 867
Size: 700 167
AleI CACnn'nnGTG
Cuts at: 0 574 867
Size: 574 293
AlwNI CAG_nnn'CTG
Cuts at: 0 230 530 633 646 867
Size: 230 300 103 13 221
Fragments arranged by size:
300 230 221 103 13
ApaI G_GGCC'C
Cuts at: 0 449 867
Size: 449 418
BanI G'GyrC_C
Cuts at: 0 269 568 867
Size: 269 299 299
Fragments arranged by size:
299 299 269
BanII G_rGCy'C
Cuts at: 0 449 522 867
Size: 449 73 345
Fragments arranged by size:
449 345 73
BciVI GTATCCnnnnn_n'
Cuts at: 0 4 867
Size: 4 863
BglII A'GATC_T
Cuts at: 0 137 867
Size: 137 730
Bme1580I G_kGCm'C
Cuts at: 0 449 867
Size: 449 418
BmrI ACTGGGnnnn_n'
Cuts at: 0 289 867
Size: 289 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 867
Size: 775 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 867
Size: 59 808
BsaWI w'CCGG_w
Cuts at: 0 500 867
Size: 500 367
BseYI C'CCAG_C
Cuts at: 0 592 633 867
Size: 592 41 234
Fragments arranged by size:
592 234 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 867
Size: 785 82
BsiEI CG_ry'CG
Cuts at: 0 501 867
Size: 501 366
BsmI GAATG_Cn'
Cuts at: 0 842 867
Size: 842 25
Bsp1286I G_dGCh'C
Cuts at: 0 449 522 867
Size: 449 73 345
Fragments arranged by size:
449 345 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 867
Size: 239 628
BstXI CCAn_nnnn'nTGG
Cuts at: 0 834 867
Size: 834 33
BstYI r'GATC_y
Cuts at: 0 137 867
Size: 137 730
BtsI GCAGTG_nn'
Cuts at: 0 35 867
Size: 35 832
EaeI y'GGCC_r
Cuts at: 0 195 836 867
Size: 195 641 31
Fragments arranged by size:
641 195 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 867
Size: 775 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 440 757 867
Size: 440 317 110
Fragments arranged by size:
440 317 110
EcoO109I rG'GnC_Cy
Cuts at: 0 227 445 589 867
Size: 227 218 144 278
Fragments arranged by size:
278 227 218 144
EcoRV GAT'ATC
Cuts at: 0 384 867
Size: 384 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 686 718 867
Size: 686 32 149
Fragments arranged by size:
686 149 32
KpnI G_GTAC'C
Cuts at: 0 273 867
Size: 273 594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 867
Size: 357 510
MscI TGG'CCA
Cuts at: 0 838 867
Size: 838 29
MslI CAynn'nnrTG
Cuts at: 0 260 574 832 867
Size: 260 314 258 35
Fragments arranged by size:
314 260 258 35
MspA1I CmG'CkG
Cuts at: 0 787 867
Size: 787 80
NspI r_CATG'y
Cuts at: 0 261 867
Size: 261 606
PciI A'CATG_T
Cuts at: 0 257 867
Size: 257 610
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 867
Size: 315 552
PsiI TTA'TAA
Cuts at: 0 29 867
Size: 29 838
PspOMI G'GGCC_C
Cuts at: 0 445 867
Size: 445 422
PstI C_TGCA'G
Cuts at: 0 42 629 867
Size: 42 587 238
Fragments arranged by size:
587 238 42
PvuII CAG'CTG
Cuts at: 0 787 867
Size: 787 80
SexAI A'CCwGG_T
Cuts at: 0 414 867
Size: 414 453
SfcI C'TryA_G
Cuts at: 0 38 559 625 867
Size: 38 521 66 242
Fragments arranged by size:
521 242 66 38
SmlI C'TyrA_G
Cuts at: 0 74 867
Size: 74 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 867
Size: 307 560
TatI w'GTAC_w
Cuts at: 0 175 658 867
Size: 175 483 209
Fragments arranged by size:
483 209 175
XmnI GAAnn'nnTTC
Cuts at: 0 47 867
Size: 47 820
Enzymes that do cut:
Acc65I AflIII AhdI AleI AlwNI ApaI BanI BanII
BciVI BglII Bme1580I BmrI BpmI BpuEI BsaWI BseYI
BsgI BsiEI BsmI Bsp1286I BspMI BstXI BstYI BtsI
EaeI Eco57MI EcoNI EcoO109I EcoRV Hin4I KpnI MmeI
MscI MslI MspA1I NspI PciI PflMI PsiI PspOMI
PstI PvuII SexAI SfcI SmlI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoRI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI