Restriction map for
pFN21AE1776
(Linear) (Six-base) MAPSORT of: KIEE1776.seq Check: 4938 from: 1 to: 852
KIEE1776.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 394 852
Size: 394 458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 852
Size: 478 374
AflIII A'CryG_T
Cuts at: 0 287 852
Size: 287 565
AlwNI CAG_nnn'CTG
Cuts at: 0 403 852
Size: 403 449
BanI G'GyrC_C
Cuts at: 0 70 807 852
Size: 70 737 45
Fragments arranged by size:
737 70 45
BanII G_rGCy'C
Cuts at: 0 24 34 152 852
Size: 24 10 118 700
Fragments arranged by size:
700 118 24 10
BbeI G_GCGC'C
Cuts at: 0 74 852
Size: 74 778
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 495 852
Size: 254 241 357
Fragments arranged by size:
357 254 241
BglI GCCn_nnn'nGGC
Cuts at: 0 116 852
Size: 116 736
Bme1580I G_kGCm'C
Cuts at: 0 812 852
Size: 812 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 675 811 852
Size: 409 266 136 41
Fragments arranged by size:
409 266 136 41
BsaAI yAC'GTr
Cuts at: 0 141 561 852
Size: 141 420 291
Fragments arranged by size:
420 291 141
BsaBI GATnn'nnATC
Cuts at: 0 199 852
Size: 199 653
BsaHI Gr'CG_yC
Cuts at: 0 71 698 852
Size: 71 627 154
Fragments arranged by size:
627 154 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 852
Size: 277 30 545
Fragments arranged by size:
545 277 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 796 852
Size: 796 56
BseYI C'CCAG_C
Cuts at: 0 66 852
Size: 66 786
BsiEI CG_ry'CG
Cuts at: 0 181 738 852
Size: 181 557 114
Fragments arranged by size:
557 181 114
BsiHKAI G_wGCw'C
Cuts at: 0 83 295 852
Size: 83 212 557
Fragments arranged by size:
557 212 83
BsmBI CGTCTCn'nnnn_
Cuts at: 0 49 852
Size: 49 803
Bsp1286I G_dGCh'C
Cuts at: 0 24 34 83 152 295 812 852
Size: 24 10 49 69 143 517 40
Fragments arranged by size:
517 143 69 49 40 24 10
BsrFI r'CCGG_y
Cuts at: 0 106 852
Size: 106 746
BssHII G'CGCG_C
Cuts at: 0 102 852
Size: 102 750
BstEII G'GTnAC_C
Cuts at: 0 597 852
Size: 597 255
BstZ17I GTA'TAC
Cuts at: 0 395 852
Size: 395 457
BtgI C'CryG_G
Cuts at: 0 812 852
Size: 812 40
EaeI y'GGCC_r
Cuts at: 0 143 178 448 527 852
Size: 143 35 270 79 325
Fragments arranged by size:
325 270 143 79 35
EagI C'GGCC_G
Cuts at: 0 178 852
Size: 178 674
EarI CTCTTCn'nnn_
Cuts at: 0 796 852
Size: 796 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 14 852
Size: 14 838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 478 675 811 852
Size: 409 69 197 136 41
Fragments arranged by size:
409 197 136 69 41
HaeII r_GCGC'y
Cuts at: 0 74 136 852
Size: 74 62 716
Fragments arranged by size:
716 74 62
KasI G'GCGC_C
Cuts at: 0 70 852
Size: 70 782
MscI TGG'CCA
Cuts at: 0 450 852
Size: 450 402
MspA1I CmG'CkG
Cuts at: 0 93 541 852
Size: 93 448 311
Fragments arranged by size:
448 311 93
NaeI GCC'GGC
Cuts at: 0 108 852
Size: 108 744
NarI GG'CG_CC
Cuts at: 0 71 852
Size: 71 781
NgoMIV G'CCGG_C
Cuts at: 0 106 852
Size: 106 746
NspI r_CATG'y
Cuts at: 0 291 852
Size: 291 561
PciI A'CATG_T
Cuts at: 0 287 852
Size: 287 565
PmlI CAC'GTG
Cuts at: 0 561 852
Size: 561 291
PshAI GACnn'nnGTC
Cuts at: 0 334 852
Size: 334 518
PstI C_TGCA'G
Cuts at: 0 116 852
Size: 116 736
PvuI CG_AT'CG
Cuts at: 0 738 852
Size: 738 114
PvuII CAG'CTG
Cuts at: 0 541 852
Size: 541 311
SbfI CC_TGCA'GG
Cuts at: 0 116 852
Size: 116 736
SfcI C'TryA_G
Cuts at: 0 112 852
Size: 112 740
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 116 852
Size: 116 736
SfoI GGC'GCC
Cuts at: 0 72 852
Size: 72 780
StyI C'CwwG_G
Cuts at: 0 359 852
Size: 359 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 656 688 852
Size: 656 32 164
Fragments arranged by size:
656 164 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 852
Size: 63 789
Enzymes that do cut:
AccI AcuI AflIII AlwNI BanI BanII BbeI BbsI
BglI Bme1580I BpmI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BstEII
BstZ17I BtgI EaeI EagI EarI EciI Eco57MI HaeII
KasI MscI MspA1I NaeI NarI NgoMIV NspI PciI
PmlI PshAI PstI PvuI PvuII SbfI SfcI SfiI
SfoI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaWI BsgI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MslI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PpuMI PsiI PspOMI PspXI PsrI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI