Restriction map for
pFN21AE1774
(Linear) (Six-base) MAPSORT of: KIEE1774.seq Check: 3367 from: 1 to: 843
KIEE1774.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 566 843
Size: 566 277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 843
Size: 670 32 141
Fragments arranged by size:
670 141 32
ApoI r'AATT_y
Cuts at: 0 595 843
Size: 595 248
AvaI C'yCGr_G
Cuts at: 0 470 843
Size: 470 373
BamHI G'GATC_C
Cuts at: 0 466 843
Size: 466 377
BanI G'GyrC_C
Cuts at: 0 779 843
Size: 779 64
BbeI G_GCGC'C
Cuts at: 0 783 843
Size: 783 60
BclI T'GATC_A
Cuts at: 0 518 843
Size: 518 325
BglI GCCn_nnn'nGGC
Cuts at: 0 538 843
Size: 538 305
Bme1580I G_kGCm'C
Cuts at: 0 227 843
Size: 227 616
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 804 843
Size: 200 604 39
Fragments arranged by size:
604 200 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 843
Size: 778 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 843
Size: 287 556
BsaBI GATnn'nnATC
Cuts at: 0 100 843
Size: 100 743
BsaHI Gr'CG_yC
Cuts at: 0 4 18 780 843
Size: 4 14 762 63
Fragments arranged by size:
762 63 14 4
BsaWI w'CCGG_w
Cuts at: 0 21 550 843
Size: 21 529 293
Fragments arranged by size:
529 293 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 843
Size: 670 30 143
Fragments arranged by size:
670 143 30
BseYI C'CCAG_C
Cuts at: 0 118 303 843
Size: 118 185 540
Fragments arranged by size:
540 185 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 843
Size: 394 449
BsiEI CG_ry'CG
Cuts at: 0 15 822 843
Size: 15 807 21
Fragments arranged by size:
807 21 15
Bsp1286I G_dGCh'C
Cuts at: 0 227 843
Size: 227 616
BspEI T'CCGG_A
Cuts at: 0 21 843
Size: 21 822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 305 843
Size: 176 129 538
Fragments arranged by size:
538 176 129
BsrBI CCG'CTC
Cuts at: 0 771 843
Size: 771 72
BsrFI r'CCGG_y
Cuts at: 0 14 817 843
Size: 14 803 26
Fragments arranged by size:
803 26 14
BstYI r'GATC_y
Cuts at: 0 466 843
Size: 466 377
BtgI C'CryG_G
Cuts at: 0 9 843
Size: 9 834
BtsI GCAGTG_nn'
Cuts at: 0 382 843
Size: 382 461
EaeI y'GGCC_r
Cuts at: 0 12 819 843
Size: 12 807 24
Fragments arranged by size:
807 24 12
EagI C'GGCC_G
Cuts at: 0 12 819 843
Size: 12 807 24
Fragments arranged by size:
807 24 12
EarI CTCTTCn'nnn_
Cuts at: 0 733 843
Size: 733 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 47 843
Size: 47 796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 843
Size: 778 65
EcoRV GAT'ATC
Cuts at: 0 102 246 843
Size: 102 144 597
Fragments arranged by size:
597 144 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 843
Size: 561 282
HaeII r_GCGC'y
Cuts at: 0 423 783 843
Size: 423 360 60
Fragments arranged by size:
423 360 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 639 671 726 758 843
Size: 639 32 55 32 85
Fragments arranged by size:
639 85 55 32 32
KasI G'GCGC_C
Cuts at: 0 779 843
Size: 779 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 843
Size: 491 352
MspA1I CmG'CkG
Cuts at: 0 11 486 843
Size: 11 475 357
Fragments arranged by size:
475 357 11
NaeI GCC'GGC
Cuts at: 0 16 843
Size: 16 827
NarI GG'CG_CC
Cuts at: 0 780 843
Size: 780 63
NgoMIV G'CCGG_C
Cuts at: 0 14 843
Size: 14 829
PasI CC'CwG_GG
Cuts at: 0 674 843
Size: 674 169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 843
Size: 670 32 141
Fragments arranged by size:
670 141 32
PstI C_TGCA'G
Cuts at: 0 485 843
Size: 485 358
PvuII CAG'CTG
Cuts at: 0 486 843
Size: 486 357
SacII CC_GC'GG
Cuts at: 0 12 843
Size: 12 831
SfcI C'TryA_G
Cuts at: 0 481 843
Size: 481 362
SfoI GGC'GCC
Cuts at: 0 781 843
Size: 781 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 293 741 823 843
Size: 222 71 448 82 20
Fragments arranged by size:
448 222 82 71 20
TatI w'GTAC_w
Cuts at: 0 425 812 843
Size: 425 387 31
Fragments arranged by size:
425 387 31
Enzymes that do cut:
AccI AloI ApoI AvaI BamHI BanI BbeI BclI
BglI Bme1580I BmrI BpmI BsaI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI Bsp1286I BspEI BspMI BsrBI
BsrFI BstYI BtgI BtsI EaeI EagI EarI EciI
Eco57MI EcoRV FalI HaeII Hin4I KasI MmeI MspA1I
NaeI NarI NgoMIV PasI PpiI PstI PvuII SacII
SfcI SfoI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BanII BbsI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BseRI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoO109I EcoRI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI