(Linear) (Six-base) MAPSORT of: KIEE1773.seq  Check: 4142  from: 1  to: 840



KIEE1773.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AccI GT'mk_AC

Cuts at:      0     195     511     840 
   Size:        195     316     329

  Fragments arranged by size: 

                 329     316     195
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     840 
   Size:        332     508
 
AlwNI CAG_nnn'CTG

Cuts at:      0     699     840 
   Size:        699     141
 
ApoI r'AATT_y

Cuts at:      0     133     832     840 
   Size:        133     699       8

  Fragments arranged by size: 

                 699     133       8
 
AvaI C'yCGr_G

Cuts at:      0     606     840 
   Size:        606     234
 
BanII G_rGCy'C

Cuts at:      0      41     613     840 
   Size:         41     572     227

  Fragments arranged by size: 

                 572     227      41
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      25      59     840 
   Size:         25      34     781

  Fragments arranged by size: 

                 781      34      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     840 
   Size:        307     533
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     479     509     840 
   Size:        479      30     331

  Fragments arranged by size: 

                 479     331      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     500     840 
   Size:        500     340
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     293     613     840 
   Size:         41     252     320     227

  Fragments arranged by size: 

                 320     252     227      41
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     293     613     840 
   Size:         41     252     320     227

  Fragments arranged by size: 

                 320     252     227      41
 
BstZ17I GTA'TAC

Cuts at:      0     196     840 
   Size:        196     644
 
DraI TTT'AAA

Cuts at:      0     779     794     840 
   Size:        779      15      46

  Fragments arranged by size: 

                 779      46      15
 
EcoICRI GAG'CTC

Cuts at:      0      39     611     840 
   Size:         39     572     229

  Fragments arranged by size: 

                 572     229      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     840 
   Size:        332     508
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      15     840 
   Size:         15     825
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     436     468     662     694     840 
   Size:        436      32     194      32     146

  Fragments arranged by size: 

                 436     194     146      32      32
 
HindIII A'AGCT_T

Cuts at:      0     544     840 
   Size:        544     296
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     510     542     840 
   Size:        510      32     298

  Fragments arranged by size: 

                 510     298      32
 
PspXI vC'TCGA_Gb

Cuts at:      0     606     840 
   Size:        606     234
 
PstI C_TGCA'G

Cuts at:      0     361     840 
   Size:        361     479
 
SacI G_AGCT'C

Cuts at:      0      41     613     840 
   Size:         41     572     227

  Fragments arranged by size: 

                 572     227      41
 
SfcI C'TryA_G

Cuts at:      0     357     666     840 
   Size:        357     309     174

  Fragments arranged by size: 

                 357     309     174
 
SmlI C'TyrA_G

Cuts at:      0     286     606     840 
   Size:        286     320     234

  Fragments arranged by size: 

                 320     286     234
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      49     385     434     840 
   Size:         49     336      49     406

  Fragments arranged by size: 

                 406     336      49      49
 
TatI w'GTAC_w

Cuts at:      0     353     840 
   Size:        353     487
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     283     315     479     511     840 
   Size:        283      32     164      32     329

  Fragments arranged by size: 

                 329     283     164      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     475     840 
   Size:        475     365
 
XhoI C'TCGA_G

Cuts at:      0     606     840 
   Size:        606     234
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AvaI    BanII     BcgI    BpuEI
    BsaXI    BseRI  BsiHKAI Bsp1286I  BstZ17I     DraI  EcoICRI  Eco57MI
    EcoNI    Hin4I  HindIII     PpiI    PspXI     PstI     SacI     SfcI
     SmlI    TaqII     TatI     TstI  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     XbaI     XcmI     XmaI     XmnI     ZraI