Restriction map for
pFN21AE1770
(Linear) (Six-base) MAPSORT of: KIEE1770.seq Check: 709 from: 1 to: 831
KIEE1770.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 130 285 831
Size: 130 155 546
Fragments arranged by size:
546 155 130
AccI GT'mk_AC
Cuts at: 0 575 740 831
Size: 575 165 91
Fragments arranged by size:
575 165 91
AflIII A'CryG_T
Cuts at: 0 599 831
Size: 599 232
ApaI G_GGCC'C
Cuts at: 0 661 826 831
Size: 661 165 5
Fragments arranged by size:
661 165 5
AvrII C'CTAG_G
Cuts at: 0 255 270 831
Size: 255 15 561
Fragments arranged by size:
561 255 15
BamHI G'GATC_C
Cuts at: 0 770 831
Size: 770 61
BanI G'GyrC_C
Cuts at: 0 460 487 831
Size: 460 27 344
Fragments arranged by size:
460 344 27
BanII G_rGCy'C
Cuts at: 0 245 661 826 831
Size: 245 416 165 5
Fragments arranged by size:
416 245 165 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 59 831
Size: 59 772
Bme1580I G_kGCm'C
Cuts at: 0 490 661 826 831
Size: 490 171 165 5
Fragments arranged by size:
490 171 165 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 831
Size: 666 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 831
Size: 403 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 831
Size: 738 93
BsaWI w'CCGG_w
Cuts at: 0 335 831
Size: 335 496
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 376 406 831
Size: 179 30 167 30 425
Fragments arranged by size:
425 179 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 831
Size: 574 257
BseYI C'CCAG_C
Cuts at: 0 482 800 831
Size: 482 318 31
Fragments arranged by size:
482 318 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 699 831
Size: 699 132
Bsp1286I G_dGCh'C
Cuts at: 0 245 490 661 826 831
Size: 245 245 171 165 5
Fragments arranged by size:
245 245 171 165 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 130 285 831
Size: 130 155 546
Fragments arranged by size:
546 155 130
BsrFI r'CCGG_y
Cuts at: 0 610 728 831
Size: 610 118 103
Fragments arranged by size:
610 118 103
BstYI r'GATC_y
Cuts at: 0 770 831
Size: 770 61
BtsI GCAGTG_nn'
Cuts at: 0 215 831
Size: 215 616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 831
Size: 403 428
EcoO109I rG'GnC_Cy
Cuts at: 0 210 657 658 822 831
Size: 210 447 1 164 9
Fragments arranged by size:
447 210 164 9 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 376 408 831
Size: 179 32 165 32 423
Fragments arranged by size:
423 179 165 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 831
Size: 567 264
MslI CAynn'nnrTG
Cuts at: 0 365 831
Size: 365 466
NaeI GCC'GGC
Cuts at: 0 612 831
Size: 612 219
NgoMIV G'CCGG_C
Cuts at: 0 610 831
Size: 610 221
NspI r_CATG'y
Cuts at: 0 603 831
Size: 603 228
PciI A'CATG_T
Cuts at: 0 599 831
Size: 599 232
PpuMI rG'GwC_Cy
Cuts at: 0 210 831
Size: 210 621
PspOMI G'GGCC_C
Cuts at: 0 657 822 831
Size: 657 165 9
Fragments arranged by size:
657 165 9
PstI C_TGCA'G
Cuts at: 0 392 831
Size: 392 439
SfcI C'TryA_G
Cuts at: 0 388 831
Size: 388 443
SmlI C'TyrA_G
Cuts at: 0 753 831
Size: 753 78
SpeI A'CTAG_T
Cuts at: 0 159 831
Size: 159 672
StyI C'CwwG_G
Cuts at: 0 35 71 255 270 831
Size: 35 36 184 15 561
Fragments arranged by size:
561 184 36 35 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 831
Size: 364 467
Enzymes that do cut:
AarI AccI AflIII ApaI AvrII BamHI BanI BanII
BbsI Bme1580I BplI BpmI BpuEI BsaWI BsaXI BseRI
BseYI BsmBI Bsp1286I BspMI BsrFI BstYI BtsI Eco57MI
EcoO109I Hin4I MmeI MslI NaeI NgoMIV NspI PciI
PpuMI PspOMI PstI SfcI SmlI SpeI StyI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI