(Linear) (Six-base) MAPSORT of: KIEE1765.seq  Check: 9452  from: 1  to: 810



KIEE1765.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AccI GT'mk_AC

Cuts at:      0     265     755     810 
   Size:        265     490      55

  Fragments arranged by size: 

                 490     265      55
 
ApoI r'AATT_y

Cuts at:      0      32     344     768     810 
   Size:         32     312     424      42

  Fragments arranged by size: 

                 424     312      42      32
 
BanII G_rGCy'C

Cuts at:      0     614     810 
   Size:        614     196
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     449     810 
   Size:        449     361
 
BclI T'GATC_A

Cuts at:      0     390     810 
   Size:        390     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     810 
   Size:        728      82
 
BsaBI GATnn'nnATC

Cuts at:      0     734     810 
   Size:        734      76
 
BseYI C'CCAG_C

Cuts at:      0      84     810 
   Size:         84     726
 
BsiHKAI G_wGCw'C

Cuts at:      0     321     614     810 
   Size:        321     293     196

  Fragments arranged by size: 

                 321     293     196
 
BsmI GAATG_Cn'

Cuts at:      0     697     810 
   Size:        697     113
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     614     810 
   Size:        321     293     196

  Fragments arranged by size: 

                 321     293     196
 
BspHI T'CATG_A

Cuts at:      0     324     810 
   Size:        324     486
 
BsrBI CCG'CTC

Cuts at:      0     143     810 
   Size:        143     667
 
BstEII G'GTnAC_C

Cuts at:      0     549     810 
   Size:        549     261
 
BtsI GCAGTG_nn'

Cuts at:      0     631     810 
   Size:        631     179
 
DraIII CAC_nnn'GTG

Cuts at:      0     606     810 
   Size:        606     204
 
EarI CTCTTCn'nnn_

Cuts at:      0     765     810 
   Size:        765      45
 
EcoICRI GAG'CTC

Cuts at:      0     612     810 
   Size:        612     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     810 
   Size:        728      82
 
HincII GTy'rAC

Cuts at:      0     256     810 
   Size:        256     554
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     810 
   Size:        698     112
 
MslI CAynn'nnrTG

Cuts at:      0     620     690     810 
   Size:        620      70     120

  Fragments arranged by size: 

                 620     120      70
 
NspI r_CATG'y

Cuts at:      0     586     810 
   Size:        586     224
 
PfoI T'CCnGG_A

Cuts at:      0     705     810 
   Size:        705     105
 
SacI G_AGCT'C

Cuts at:      0     614     810 
   Size:        614     196
 
SphI G_CATG'C

Cuts at:      0     586     810 
   Size:        586     224
 
StuI AGG'CCT

Cuts at:      0     169     201     646     810 
   Size:        169      32     445     164

  Fragments arranged by size: 

                 445     169     164      32
 
TatI w'GTAC_w

Cuts at:      0     125     775     810 
   Size:        125     650      35

  Fragments arranged by size: 

                 650     125      35
 

 Enzymes that do cut:

     AccI     ApoI    BanII     BbsI     BclI     BpmI    BsaBI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI   BstEII     BtsI   DraIII
     EarI  EcoICRI  Eco57MI   HincII     MmeI     MslI     NspI     PfoI
     SacI     SphI     StuI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI