Restriction map for
pFN21AE1759
(Linear) (Six-base) MAPSORT of: KIEE1759.seq Check: 2719 from: 1 to: 789
KIEE1759.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 313 789
Size: 133 180 476
Fragments arranged by size:
476 180 133
ApaI G_GGCC'C
Cuts at: 0 788 789
Size: 788 1
ApoI r'AATT_y
Cuts at: 0 334 628 789
Size: 334 294 161
Fragments arranged by size:
334 294 161
AvaI C'yCGr_G
Cuts at: 0 97 789
Size: 97 692
BanII G_rGCy'C
Cuts at: 0 788 789
Size: 788 1
BglI GCCn_nnn'nGGC
Cuts at: 0 155 789
Size: 155 634
Bme1580I G_kGCm'C
Cuts at: 0 788 789
Size: 788 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 789
Size: 26 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 667 789
Size: 173 494 122
Fragments arranged by size:
494 173 122
Bpu10I CC'TnA_GC
Cuts at: 0 189 789
Size: 189 600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 544 789
Size: 400 144 245
Fragments arranged by size:
400 245 144
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 388 397 789
Size: 289 99 9 392
Fragments arranged by size:
392 289 99 9
BseYI C'CCAG_C
Cuts at: 0 324 789
Size: 324 465
BsiEI CG_ry'CG
Cuts at: 0 264 366 789
Size: 264 102 423
Fragments arranged by size:
423 264 102
BsiHKAI G_wGCw'C
Cuts at: 0 416 482 789
Size: 416 66 307
Fragments arranged by size:
416 307 66
Bsp1286I G_dGCh'C
Cuts at: 0 416 482 788 789
Size: 416 66 306 1
Fragments arranged by size:
416 306 66 1
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 789
Size: 45 744
BstEII G'GTnAC_C
Cuts at: 0 579 789
Size: 579 210
BtgI C'CryG_G
Cuts at: 0 494 789
Size: 494 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 437 789
Size: 437 352
BtsI GCAGTG_nn'
Cuts at: 0 553 789
Size: 553 236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 549 584 789
Size: 549 35 205
Fragments arranged by size:
549 205 35
DraIII CAC_nnn'GTG
Cuts at: 0 579 789
Size: 579 210
EaeI y'GGCC_r
Cuts at: 0 363 491 570 789
Size: 363 128 79 219
Fragments arranged by size:
363 219 128 79
EagI C'GGCC_G
Cuts at: 0 363 789
Size: 363 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 173 313 667 789
Size: 133 40 140 354 122
Fragments arranged by size:
354 140 133 122 40
EcoO109I rG'GnC_Cy
Cuts at: 0 193 308 789
Size: 193 115 481
Fragments arranged by size:
481 193 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 138 789
Size: 27 111 651
Fragments arranged by size:
651 111 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 789
Size: 172 32 585
Fragments arranged by size:
585 172 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 789
Size: 275 514
MscI TGG'CCA
Cuts at: 0 493 572 789
Size: 493 79 217
Fragments arranged by size:
493 217 79
MspA1I CmG'CkG
Cuts at: 0 328 362 400 444 789
Size: 328 34 38 44 345
Fragments arranged by size:
345 328 44 38 34
NcoI C'CATG_G
Cuts at: 0 494 789
Size: 494 295
PasI CC'CwG_GG
Cuts at: 0 50 789
Size: 50 739
PfoI T'CCnGG_A
Cuts at: 0 674 789
Size: 674 115
PpuMI rG'GwC_Cy
Cuts at: 0 193 789
Size: 193 596
PshAI GACnn'nnGTC
Cuts at: 0 586 789
Size: 586 203
PspOMI G'GGCC_C
Cuts at: 0 784 789
Size: 784 5
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 689 721 789
Size: 689 32 68
Fragments arranged by size:
689 68 32
SexAI A'CCwGG_T
Cuts at: 0 575 789
Size: 575 214
SfcI C'TryA_G
Cuts at: 0 72 510 789
Size: 72 438 279
Fragments arranged by size:
438 279 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 155 789
Size: 155 634
SmlI C'TyrA_G
Cuts at: 0 415 559 789
Size: 415 144 230
Fragments arranged by size:
415 230 144
StuI AGG'CCT
Cuts at: 0 158 789
Size: 158 631
StyI C'CwwG_G
Cuts at: 0 162 494 789
Size: 162 332 295
Fragments arranged by size:
332 295 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 789
Size: 57 732
XmnI GAAnn'nnTTC
Cuts at: 0 40 789
Size: 40 749
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanII BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BstAPI BstEII BtgI BtgZI BtsI CspCI DraIII EaeI
EagI Eco57MI EcoO109I FalI Hin4I MmeI MscI MspA1I
NcoI PasI PfoI PpuMI PshAI PspOMI PsrI SexAI
SfcI SfiI SmlI StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI
BstZ17I Bsu36I ClaI DraI DrdI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI ZraI