Restriction map for
pFN21AE1755
(Linear) (Six-base) MAPSORT of: KIEE1755.seq Check: 6832 from: 1 to: 783
KIEE1755.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
Acc65I G'GTAC_C
Cuts at: 0 701 783
Size: 701 82
ApoI r'AATT_y
Cuts at: 0 764 783
Size: 764 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 693 726 783
Size: 693 33 57
Fragments arranged by size:
693 57 33
BamHI G'GATC_C
Cuts at: 0 401 783
Size: 401 382
BanI G'GyrC_C
Cuts at: 0 568 701 783
Size: 568 133 82
Fragments arranged by size:
568 133 82
BanII G_rGCy'C
Cuts at: 0 27 547 783
Size: 27 520 236
Fragments arranged by size:
520 236 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 579 703 783
Size: 579 124 80
Fragments arranged by size:
579 124 80
BbvCI CC'TCA_GC
Cuts at: 0 185 227 783
Size: 185 42 556
Fragments arranged by size:
556 185 42
Bme1580I G_kGCm'C
Cuts at: 0 326 783
Size: 326 457
BmgBI CAC'GTC
Cuts at: 0 432 783
Size: 432 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 400 783
Size: 400 383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 606 783
Size: 89 517 177
Fragments arranged by size:
517 177 89
Bpu10I CC'TnA_GC
Cuts at: 0 185 227 783
Size: 185 42 556
Fragments arranged by size:
556 185 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 783
Size: 11 772
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 372 402 783
Size: 372 30 381
Fragments arranged by size:
381 372 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 107 783
Size: 67 40 676
Fragments arranged by size:
676 67 40
BseYI C'CCAG_C
Cuts at: 0 352 783
Size: 352 431
BsiHKAI G_wGCw'C
Cuts at: 0 285 470 512 783
Size: 285 185 42 271
Fragments arranged by size:
285 271 185 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 438 783
Size: 438 345
Bsp1286I G_dGCh'C
Cuts at: 0 27 285 326 470 512 547 783
Size: 27 258 41 144 42 35 236
Fragments arranged by size:
258 236 144 42 41 35 27
BsrDI GCAATG_nn'
Cuts at: 0 548 783
Size: 548 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 783
Size: 321 462
BstXI CCAn_nnnn'nTGG
Cuts at: 0 398 630 783
Size: 398 232 153
Fragments arranged by size:
398 232 153
BstYI r'GATC_y
Cuts at: 0 401 783
Size: 401 382
BtgI C'CryG_G
Cuts at: 0 606 657 739 783
Size: 606 51 82 44
Fragments arranged by size:
606 82 51 44
DraIII CAC_nnn'GTG
Cuts at: 0 321 783
Size: 321 462
EaeI y'GGCC_r
Cuts at: 0 287 783
Size: 287 496
EarI CTCTTCn'nnn_
Cuts at: 0 68 109 783
Size: 68 41 674
Fragments arranged by size:
674 68 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 606 783
Size: 89 517 177
Fragments arranged by size:
517 177 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 624 783
Size: 624 159
EcoO109I rG'GnC_Cy
Cuts at: 0 33 132 160 189 783
Size: 33 99 28 29 594
Fragments arranged by size:
594 99 33 29 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 52 783
Size: 52 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 569 601 783
Size: 418 32 119 32 182
Fragments arranged by size:
418 182 119 32 32
KpnI G_GTAC'C
Cuts at: 0 705 783
Size: 705 78
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 783
Size: 233 550
MscI TGG'CCA
Cuts at: 0 289 783
Size: 289 494
MslI CAynn'nnrTG
Cuts at: 0 515 783
Size: 515 268
MspA1I CmG'CkG
Cuts at: 0 82 651 783
Size: 82 569 132
Fragments arranged by size:
569 132 82
PasI CC'CwG_GG
Cuts at: 0 136 538 783
Size: 136 402 245
Fragments arranged by size:
402 245 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 142 252 783
Size: 142 110 531
Fragments arranged by size:
531 142 110
PfoI T'CCnGG_A
Cuts at: 0 422 622 783
Size: 422 200 161
Fragments arranged by size:
422 200 161
PstI C_TGCA'G
Cuts at: 0 202 577 783
Size: 202 375 206
Fragments arranged by size:
375 206 202
PvuII CAG'CTG
Cuts at: 0 82 783
Size: 82 701
SapI GCTCTTCn'nnn_
Cuts at: 0 68 783
Size: 68 715
SfcI C'TryA_G
Cuts at: 0 198 573 783
Size: 198 375 210
Fragments arranged by size:
375 210 198
SmlI C'TyrA_G
Cuts at: 0 26 783
Size: 26 757
SspI AAT'ATT
Cuts at: 0 271 783
Size: 271 512
StuI AGG'CCT
Cuts at: 0 377 783
Size: 377 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 783
Size: 372 32 379
Fragments arranged by size:
379 372 32
Tth111I GACn'n_nGTC
Cuts at: 0 583 783
Size: 583 200
XbaI T'CTAG_A
Cuts at: 0 411 783
Size: 411 372
Enzymes that do cut:
Acc65I ApoI BaeI BamHI BanI BanII BbsI BbvCI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BsrDI BstAPI BstXI BstYI
BtgI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI
Hin4I KpnI MmeI MscI MslI MspA1I PasI PflMI
PfoI PstI PvuII SapI SfcI SmlI SspI StuI
TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StyI SwaI TaqII TatI XcmI XhoI XmaI XmnI
ZraI