Restriction map for
pFN21AE1752
(Linear) (Six-base) MAPSORT of: KIEE1752.seq Check: 7769 from: 1 to: 771
KIEE1752.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 657 771
Size: 328 329 114
Fragments arranged by size:
329 328 114
AflIII A'CryG_T
Cuts at: 0 734 771
Size: 734 37
AleI CACnn'nnGTG
Cuts at: 0 721 771
Size: 721 50
AlwNI CAG_nnn'CTG
Cuts at: 0 686 771
Size: 686 85
ApaI G_GGCC'C
Cuts at: 0 45 629 771
Size: 45 584 142
Fragments arranged by size:
584 142 45
ApaLI G'TGCA_C
Cuts at: 0 166 771
Size: 166 605
AvaI C'yCGr_G
Cuts at: 0 479 618 771
Size: 479 139 153
Fragments arranged by size:
479 153 139
BanI G'GyrC_C
Cuts at: 0 470 726 771
Size: 470 256 45
Fragments arranged by size:
470 256 45
BanII G_rGCy'C
Cuts at: 0 45 242 629 771
Size: 45 197 387 142
Fragments arranged by size:
387 197 142 45
BbeI G_GCGC'C
Cuts at: 0 474 730 771
Size: 474 256 41
Fragments arranged by size:
474 256 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 8 771
Size: 8 763
BbvCI CC'TCA_GC
Cuts at: 0 223 771
Size: 223 548
BglI GCCn_nnn'nGGC
Cuts at: 0 40 771
Size: 40 731
Bme1580I G_kGCm'C
Cuts at: 0 45 170 629 771
Size: 45 125 459 142
Fragments arranged by size:
459 142 125 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 392 771
Size: 392 379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 663 771
Size: 343 320 108
Fragments arranged by size:
343 320 108
Bpu10I CC'TnA_GC
Cuts at: 0 223 771
Size: 223 548
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 771
Size: 268 503
BsaAI yAC'GTr
Cuts at: 0 735 771
Size: 735 36
BsaHI Gr'CG_yC
Cuts at: 0 471 727 771
Size: 471 256 44
Fragments arranged by size:
471 256 44
BseYI C'CCAG_C
Cuts at: 0 206 218 234 367 446 562 771
Size: 206 12 16 133 79 116 209
Fragments arranged by size:
209 206 133 116 79 16 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 672 771
Size: 453 219 99
Fragments arranged by size:
453 219 99
BsiEI CG_ry'CG
Cuts at: 0 385 584 762 766 771
Size: 385 199 178 4 5
Fragments arranged by size:
385 199 178 5 4
BsiHKAI G_wGCw'C
Cuts at: 0 170 771
Size: 170 601
Bsp1286I G_dGCh'C
Cuts at: 0 45 170 242 629 771
Size: 45 125 72 387 142
Fragments arranged by size:
387 142 125 72 45
BsrFI r'CCGG_y
Cuts at: 0 442 757 771
Size: 442 315 14
Fragments arranged by size:
442 315 14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 572 771
Size: 572 199
BstEII G'GTnAC_C
Cuts at: 0 753 771
Size: 753 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 771
Size: 723 48
BtgI C'CryG_G
Cuts at: 0 710 771
Size: 710 61
BtsI GCAGTG_nn'
Cuts at: 0 19 124 195 771
Size: 19 105 71 576
Fragments arranged by size:
576 105 71 19
EaeI y'GGCC_r
Cuts at: 0 382 440 581 656 713 759 771
Size: 382 58 141 75 57 46 12
Fragments arranged by size:
382 141 75 58 57 46 12
EagI C'GGCC_G
Cuts at: 0 382 581 759 771
Size: 382 199 178 12
Fragments arranged by size:
382 199 178 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 343 657 663 771
Size: 328 15 314 6 108
Fragments arranged by size:
328 314 108 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 744 771
Size: 744 27
EcoO109I rG'GnC_Cy
Cuts at: 0 41 42 68 626 771
Size: 41 1 26 558 145
Fragments arranged by size:
558 145 41 26 1
HaeII r_GCGC'y
Cuts at: 0 408 474 730 771
Size: 408 66 256 41
Fragments arranged by size:
408 256 66 41
KasI G'GCGC_C
Cuts at: 0 470 726 771
Size: 470 256 45
Fragments arranged by size:
470 256 45
MscI TGG'CCA
Cuts at: 0 715 771
Size: 715 56
MslI CAynn'nnrTG
Cuts at: 0 520 721 771
Size: 520 201 50
Fragments arranged by size:
520 201 50
MspA1I CmG'CkG
Cuts at: 0 210 222 348 721 771
Size: 210 12 126 373 50
Fragments arranged by size:
373 210 126 50 12
NaeI GCC'GGC
Cuts at: 0 444 771
Size: 444 327
NarI GG'CG_CC
Cuts at: 0 471 727 771
Size: 471 256 44
Fragments arranged by size:
471 256 44
NgoMIV G'CCGG_C
Cuts at: 0 442 771
Size: 442 329
NspI r_CATG'y
Cuts at: 0 525 771
Size: 525 246
PasI CC'CwG_GG
Cuts at: 0 36 771
Size: 36 735
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 213 249 414 510 771
Size: 201 12 36 165 96 261
Fragments arranged by size:
261 201 165 96 36 12
PfoI T'CCnGG_A
Cuts at: 0 739 771
Size: 739 32
PpuMI rG'GwC_Cy
Cuts at: 0 68 771
Size: 68 703
PspOMI G'GGCC_C
Cuts at: 0 41 625 771
Size: 41 584 146
Fragments arranged by size:
584 146 41
PstI C_TGCA'G
Cuts at: 0 131 771
Size: 131 640
PvuI CG_AT'CG
Cuts at: 0 766 771
Size: 766 5
PvuII CAG'CTG
Cuts at: 0 222 348 771
Size: 222 126 423
Fragments arranged by size:
423 222 126
SanDI GG'GwC_CC
Cuts at: 0 68 771
Size: 68 703
SfcI C'TryA_G
Cuts at: 0 127 771
Size: 127 644
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 40 771
Size: 40 731
SfoI GGC'GCC
Cuts at: 0 472 728 771
Size: 472 256 43
Fragments arranged by size:
472 256 43
SmaI CCC'GGG
Cuts at: 0 481 771
Size: 481 290
StuI AGG'CCT
Cuts at: 0 7 771
Size: 7 764
StyI C'CwwG_G
Cuts at: 0 590 771
Size: 590 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 185 771
Size: 168 17 586
Fragments arranged by size:
586 168 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 771
Size: 384 387
XmaI C'CCGG_G
Cuts at: 0 479 771
Size: 479 292
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrFI BstAPI BstEII BstXI BtgI BtsI EaeI
EagI Eco57MI EcoNI EcoO109I HaeII KasI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuI PvuII SanDI SfcI SfiI
SfoI SmaI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaBI BsaWI BsaXI BseRI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI