Restriction map for
pFN21AE1749
(Linear) (Six-base) MAPSORT of: KIEE1749.seq Check: 6537 from: 1 to: 756
KIEE1749.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 263 745 756
Size: 35 228 482 11
Fragments arranged by size:
482 228 35 11
AlwNI CAG_nnn'CTG
Cuts at: 0 129 286 756
Size: 129 157 470
Fragments arranged by size:
470 157 129
ApaLI G'TGCA_C
Cuts at: 0 557 756
Size: 557 199
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 129 162 756
Size: 129 33 594
Fragments arranged by size:
594 129 33
BamHI G'GATC_C
Cuts at: 0 472 756
Size: 472 284
BanI G'GyrC_C
Cuts at: 0 381 756
Size: 381 375
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 503 756
Size: 181 322 253
Fragments arranged by size:
322 253 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 118 756
Size: 84 34 638
Fragments arranged by size:
638 84 34
BglI GCCn_nnn'nGGC
Cuts at: 0 233 756
Size: 233 523
Bme1580I G_kGCm'C
Cuts at: 0 384 561 756
Size: 384 177 195
Fragments arranged by size:
384 195 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 739 756
Size: 65 674 17
Fragments arranged by size:
674 65 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 756
Size: 322 434
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 345 375 756
Size: 345 30 381
Fragments arranged by size:
381 345 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 756
Size: 715 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 756
Size: 541 215
BsiHKAI G_wGCw'C
Cuts at: 0 65 561 756
Size: 65 496 195
Fragments arranged by size:
496 195 65
Bsp1286I G_dGCh'C
Cuts at: 0 65 384 561 756
Size: 65 319 177 195
Fragments arranged by size:
319 195 177 65
BsrDI GCAATG_nn'
Cuts at: 0 195 327 538 756
Size: 195 132 211 218
Fragments arranged by size:
218 211 195 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 619 756
Size: 619 137
BstYI r'GATC_y
Cuts at: 0 472 756
Size: 472 284
Bsu36I CC'TnA_GG
Cuts at: 0 468 756
Size: 468 288
BtgI C'CryG_G
Cuts at: 0 653 756
Size: 653 103
BtsI GCAGTG_nn'
Cuts at: 0 561 756
Size: 561 195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 95 130 756
Size: 95 35 626
Fragments arranged by size:
626 95 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 65 263 739 745 756
Size: 35 30 198 476 6 11
Fragments arranged by size:
476 198 35 30 11 6
EcoO109I rG'GnC_Cy
Cuts at: 0 283 756
Size: 283 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 345 377 756
Size: 345 32 379
Fragments arranged by size:
379 345 32
MslI CAynn'nnrTG
Cuts at: 0 313 694 756
Size: 313 381 62
Fragments arranged by size:
381 313 62
NcoI C'CATG_G
Cuts at: 0 653 756
Size: 653 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 439 756
Size: 439 317
PfoI T'CCnGG_A
Cuts at: 0 581 756
Size: 581 175
SexAI A'CCwGG_T
Cuts at: 0 161 756
Size: 161 595
SmlI C'TyrA_G
Cuts at: 0 337 756
Size: 337 419
SspI AAT'ATT
Cuts at: 0 712 756
Size: 712 44
StyI C'CwwG_G
Cuts at: 0 653 756
Size: 653 103
XbaI T'CTAG_A
Cuts at: 0 363 756
Size: 363 393
Enzymes that do cut:
AcuI AlwNI ApaLI BaeI BamHI BanI BbsI BcgI
BglI Bme1580I BpmI BpuEI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BsrDI BstAPI BstYI Bsu36I BtgI BtsI CspCI
Eco57MI EcoO109I Hin4I MslI NcoI PflMI PfoI SexAI
SmlI SspI StyI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvaI AvrII BanII BbeI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI