(Linear) (Six-base) MAPSORT of: KIEE1748.seq  Check: 6902  from: 1  to: 753



KIEE1748.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     121     753 
   Size:        121     632
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     269     753 
   Size:        269     484
 
ApaI G_GGCC'C

Cuts at:      0     183     753 
   Size:        183     570
 
ApoI r'AATT_y

Cuts at:      0     409     753 
   Size:        409     344
 
BanII G_rGCy'C

Cuts at:      0     108     183     753 
   Size:        108      75     570

  Fragments arranged by size: 

                 570     108      75
 
Bme1580I G_kGCm'C

Cuts at:      0     183     291     753 
   Size:        183     108     462

  Fragments arranged by size: 

                 462     183     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     363     753 
   Size:        363     390
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     329     753 
   Size:        329     424
 
Bpu10I CC'TnA_GC

Cuts at:      0     566     753 
   Size:        566     187
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     571     753 
   Size:        571     182
 
BseYI C'CCAG_C

Cuts at:      0     101     342     575     753 
   Size:        101     241     233     178

  Fragments arranged by size: 

                 241     233     178     101
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     334     504     753 
   Size:        220     114     170     249

  Fragments arranged by size: 

                 249     220     170     114
 
BsiHKAI G_wGCw'C

Cuts at:      0      62     753 
   Size:         62     691
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     108     183     291     753 
   Size:         62      46      75     108     462

  Fragments arranged by size: 

                 462     108      75      62      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     121     167     753 
   Size:        121      46     586

  Fragments arranged by size: 

                 586     121      46
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     534     753 
   Size:        534     219
 
BstYI r'GATC_y

Cuts at:      0       9     753 
   Size:          9     744
 
BtgI C'CryG_G

Cuts at:      0     465     753 
   Size:        465     288
 
BtsI GCAGTG_nn'

Cuts at:      0     492     753 
   Size:        492     261
 
DraI TTT'AAA

Cuts at:      0     330     753 
   Size:        330     423
 
EaeI y'GGCC_r

Cuts at:      0       2     753 
   Size:          2     751
 
MslI CAynn'nnrTG

Cuts at:      0     532     753 
   Size:        532     221
 
MspA1I CmG'CkG

Cuts at:      0      68     753 
   Size:         68     685
 
PasI CC'CwG_GG

Cuts at:      0     183     753 
   Size:        183     570
 
PspOMI G'GGCC_C

Cuts at:      0     179     753 
   Size:        179     574
 
PstI C_TGCA'G

Cuts at:      0      73     753 
   Size:         73     680
 
PvuII CAG'CTG

Cuts at:      0      68     753 
   Size:         68     685
 
SfcI C'TryA_G

Cuts at:      0      69     753 
   Size:         69     684
 
StuI AGG'CCT

Cuts at:      0     193     371     753 
   Size:        193     178     382

  Fragments arranged by size: 

                 382     193     178
 
StyI C'CwwG_G

Cuts at:      0      77     283     545     753 
   Size:         77     206     262     208

  Fragments arranged by size: 

                 262     208     206      77
 
Tth111I GACn'n_nGTC

Cuts at:      0     594     753 
   Size:        594     159
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     350     753 
   Size:        350     403
 

 Enzymes that do cut:

     AarI     AlfI     ApaI     ApoI    BanII Bme1580I     BmrI     BplI
   Bpu10I    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BstXI
    BstYI     BtgI     BtsI     DraI     EaeI     MslI   MspA1I     PasI
   PspOMI     PstI    PvuII     SfcI     StuI     StyI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI