Restriction map for
pFN21AE1745
(Linear) (Six-base) MAPSORT of: KIEE1745.seq Check: 635 from: 1 to: 741
KIEE1745.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 516 538 741
Size: 71 445 22 203
Fragments arranged by size:
445 203 71 22
AlwNI CAG_nnn'CTG
Cuts at: 0 729 741
Size: 729 12
ApaI G_GGCC'C
Cuts at: 0 616 741
Size: 616 125
ApoI r'AATT_y
Cuts at: 0 145 741
Size: 145 596
BamHI G'GATC_C
Cuts at: 0 510 741
Size: 510 231
BanII G_rGCy'C
Cuts at: 0 234 475 616 741
Size: 234 241 141 125
Fragments arranged by size:
241 234 141 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 586 678 741
Size: 260 326 92 63
Fragments arranged by size:
326 260 92 63
BciVI GTATCCnnnnn_n'
Cuts at: 0 660 741
Size: 660 81
Bme1580I G_kGCm'C
Cuts at: 0 76 616 741
Size: 76 540 125
Fragments arranged by size:
540 125 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 741
Size: 27 714
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 741
Size: 292 449
BseYI C'CCAG_C
Cuts at: 0 55 443 628 741
Size: 55 388 185 113
Fragments arranged by size:
388 185 113 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 741
Size: 221 520
BsiEI CG_ry'CG
Cuts at: 0 664 741
Size: 664 77
BsiHKAI G_wGCw'C
Cuts at: 0 43 741
Size: 43 698
BsmI GAATG_Cn'
Cuts at: 0 85 741
Size: 85 656
Bsp1286I G_dGCh'C
Cuts at: 0 43 76 234 475 616 741
Size: 43 33 158 241 141 125
Fragments arranged by size:
241 158 141 125 43 33
BsrFI r'CCGG_y
Cuts at: 0 707 741
Size: 707 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 80 741
Size: 80 661
BstYI r'GATC_y
Cuts at: 0 510 725 741
Size: 510 215 16
Fragments arranged by size:
510 215 16
EarI CTCTTCn'nnn_
Cuts at: 0 323 623 741
Size: 323 300 118
Fragments arranged by size:
323 300 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 71 516 538 741
Size: 27 44 445 22 203
Fragments arranged by size:
445 203 44 27 22
EcoO109I rG'GnC_Cy
Cuts at: 0 612 613 741
Size: 612 1 128
Fragments arranged by size:
612 128 1
EcoRV GAT'ATC
Cuts at: 0 330 741
Size: 330 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 741
Size: 177 32 532
Fragments arranged by size:
532 177 32
HindIII A'AGCT_T
Cuts at: 0 268 741
Size: 268 473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 644 741
Size: 155 489 97
Fragments arranged by size:
489 155 97
MspA1I CmG'CkG
Cuts at: 0 558 741
Size: 558 183
PasI CC'CwG_GG
Cuts at: 0 639 741
Size: 639 102
PsiI TTA'TAA
Cuts at: 0 166 280 741
Size: 166 114 461
Fragments arranged by size:
461 166 114
PspOMI G'GGCC_C
Cuts at: 0 612 741
Size: 612 129
PstI C_TGCA'G
Cuts at: 0 526 734 741
Size: 526 208 7
Fragments arranged by size:
526 208 7
PvuII CAG'CTG
Cuts at: 0 558 741
Size: 558 183
SbfI CC_TGCA'GG
Cuts at: 0 734 741
Size: 734 7
SfcI C'TryA_G
Cuts at: 0 522 730 741
Size: 522 208 11
Fragments arranged by size:
522 208 11
SmlI C'TyrA_G
Cuts at: 0 271 741
Size: 271 470
StuI AGG'CCT
Cuts at: 0 520 741
Size: 520 221
StyI C'CwwG_G
Cuts at: 0 225 741
Size: 225 516
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 374 406 741
Size: 374 32 335
Fragments arranged by size:
374 335 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 741
Size: 366 375
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BamHI BanII BbsI BciVI
Bme1580I BpmI BpuEI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BsrFI BstAPI BstYI EarI Eco57MI EcoO109I EcoRV
Hin4I HindIII MmeI MspA1I PasI PsiI PspOMI PstI
PvuII SbfI SfcI SmlI StuI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI XmnI ZraI