(Linear) (Six-base) MAPSORT of: KIEE1745.seq Check: 635 from: 1 to: 741 KIEE1745.orf With 182 enzymes: SgfI * July 15, 2010 18:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 516 538 741 Size: 71 445 22 203 Fragments arranged by size: 445 203 71 22 AlwNI CAG_nnn'CTG Cuts at: 0 729 741 Size: 729 12 ApaI G_GGCC'C Cuts at: 0 616 741 Size: 616 125 ApoI r'AATT_y Cuts at: 0 145 741 Size: 145 596 BamHI G'GATC_C Cuts at: 0 510 741 Size: 510 231 BanII G_rGCy'C Cuts at: 0 234 475 616 741 Size: 234 241 141 125 Fragments arranged by size: 241 234 141 125 BbsI GAAGACnn'nnnn_ Cuts at: 0 260 586 678 741 Size: 260 326 92 63 Fragments arranged by size: 326 260 92 63 BciVI GTATCCnnnnn_n' Cuts at: 0 660 741 Size: 660 81 Bme1580I G_kGCm'C Cuts at: 0 76 616 741 Size: 76 540 125 Fragments arranged by size: 540 125 76 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 741 Size: 27 714 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 741 Size: 292 449 BseYI C'CCAG_C Cuts at: 0 55 443 628 741 Size: 55 388 185 113 Fragments arranged by size: 388 185 113 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 221 741 Size: 221 520 BsiEI CG_ry'CG Cuts at: 0 664 741 Size: 664 77 BsiHKAI G_wGCw'C Cuts at: 0 43 741 Size: 43 698 BsmI GAATG_Cn' Cuts at: 0 85 741 Size: 85 656 Bsp1286I G_dGCh'C Cuts at: 0 43 76 234 475 616 741 Size: 43 33 158 241 141 125 Fragments arranged by size: 241 158 141 125 43 33 BsrFI r'CCGG_y Cuts at: 0 707 741 Size: 707 34 BstAPI GCAn_nnn'nTGC Cuts at: 0 80 741 Size: 80 661 BstYI r'GATC_y Cuts at: 0 510 725 741 Size: 510 215 16 Fragments arranged by size: 510 215 16 EarI CTCTTCn'nnn_ Cuts at: 0 323 623 741 Size: 323 300 118 Fragments arranged by size: 323 300 118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 71 516 538 741 Size: 27 44 445 22 203 Fragments arranged by size: 445 203 44 27 22 EcoO109I rG'GnC_Cy Cuts at: 0 612 613 741 Size: 612 1 128 Fragments arranged by size: 612 128 1 EcoRV GAT'ATC Cuts at: 0 330 741 Size: 330 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 177 209 741 Size: 177 32 532 Fragments arranged by size: 532 177 32 HindIII A'AGCT_T Cuts at: 0 268 741 Size: 268 473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 155 644 741 Size: 155 489 97 Fragments arranged by size: 489 155 97 MspA1I CmG'CkG Cuts at: 0 558 741 Size: 558 183 PasI CC'CwG_GG Cuts at: 0 639 741 Size: 639 102 PsiI TTA'TAA Cuts at: 0 166 280 741 Size: 166 114 461 Fragments arranged by size: 461 166 114 PspOMI G'GGCC_C Cuts at: 0 612 741 Size: 612 129 PstI C_TGCA'G Cuts at: 0 526 734 741 Size: 526 208 7 Fragments arranged by size: 526 208 7 PvuII CAG'CTG Cuts at: 0 558 741 Size: 558 183 SbfI CC_TGCA'GG Cuts at: 0 734 741 Size: 734 7 SfcI C'TryA_G Cuts at: 0 522 730 741 Size: 522 208 11 Fragments arranged by size: 522 208 11 SmlI C'TyrA_G Cuts at: 0 271 741 Size: 271 470 StuI AGG'CCT Cuts at: 0 520 741 Size: 520 221 StyI C'CwwG_G Cuts at: 0 225 741 Size: 225 516 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 374 406 741 Size: 374 32 335 Fragments arranged by size: 374 335 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 366 741 Size: 366 375 Enzymes that do cut: AcuI AlwNI ApaI ApoI BamHI BanII BbsI BciVI Bme1580I BpmI BpuEI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrFI BstAPI BstYI EarI Eco57MI EcoO109I EcoRV Hin4I HindIII MmeI MspA1I PasI PsiI PspOMI PstI PvuII SbfI SfcI SmlI StuI StyI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI