Restriction map for
pFN21AE1740
(Linear) (Six-base) MAPSORT of: KIEE1740.seq Check: 2406 from: 1 to: 732
KIEE1740.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AccI GT'mk_AC
Cuts at: 0 329 732
Size: 329 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 732
Size: 284 448
ApaI G_GGCC'C
Cuts at: 0 74 156 732
Size: 74 82 576
Fragments arranged by size:
576 82 74
ApoI r'AATT_y
Cuts at: 0 445 732
Size: 445 287
AvaI C'yCGr_G
Cuts at: 0 256 272 732
Size: 256 16 460
Fragments arranged by size:
460 256 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 236 269 732
Size: 236 33 463
Fragments arranged by size:
463 236 33
BanI G'GyrC_C
Cuts at: 0 178 319 732
Size: 178 141 413
Fragments arranged by size:
413 178 141
BanII G_rGCy'C
Cuts at: 0 74 156 732
Size: 74 82 576
Fragments arranged by size:
576 82 74
BclI T'GATC_A
Cuts at: 0 558 732
Size: 558 174
BglI GCCn_nnn'nGGC
Cuts at: 0 167 732
Size: 167 565
BglII A'GATC_T
Cuts at: 0 401 732
Size: 401 331
BlpI GC'TnA_GC
Cuts at: 0 336 732
Size: 336 396
Bme1580I G_kGCm'C
Cuts at: 0 74 156 732
Size: 74 82 576
Fragments arranged by size:
576 82 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 622 634 732
Size: 332 290 12 98
Fragments arranged by size:
332 290 98 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 732
Size: 50 682
BsaWI w'CCGG_w
Cuts at: 0 247 732
Size: 247 485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 676 706 732
Size: 574 30 72 30 26
Fragments arranged by size:
574 72 30 30 26
BseYI C'CCAG_C
Cuts at: 0 9 732
Size: 9 723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 732
Size: 90 642
BsiHKAI G_wGCw'C
Cuts at: 0 605 732
Size: 605 127
BsmI GAATG_Cn'
Cuts at: 0 340 732
Size: 340 392
Bsp1286I G_dGCh'C
Cuts at: 0 74 156 605 732
Size: 74 82 449 127
Fragments arranged by size:
449 127 82 74
BspHI T'CATG_A
Cuts at: 0 45 732
Size: 45 687
BsrBI CCG'CTC
Cuts at: 0 335 732
Size: 335 397
BstXI CCAn_nnnn'nTGG
Cuts at: 0 430 646 732
Size: 430 216 86
Fragments arranged by size:
430 216 86
BstYI r'GATC_y
Cuts at: 0 207 401 732
Size: 207 194 331
Fragments arranged by size:
331 207 194
BstZ17I GTA'TAC
Cuts at: 0 330 732
Size: 330 402
Bsu36I CC'TnA_GG
Cuts at: 0 227 732
Size: 227 505
BtsI GCAGTG_nn'
Cuts at: 0 450 732
Size: 450 282
EarI CTCTTCn'nnn_
Cuts at: 0 529 553 732
Size: 529 24 179
Fragments arranged by size:
529 179 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 332 622 634 732
Size: 284 48 290 12 98
Fragments arranged by size:
290 284 98 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 314 732
Size: 94 220 418
Fragments arranged by size:
418 220 94
EcoO109I rG'GnC_Cy
Cuts at: 0 70 101 152 153 268 732
Size: 70 31 51 1 115 464
Fragments arranged by size:
464 115 70 51 31 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 676 708 732
Size: 574 32 70 32 24
Fragments arranged by size:
574 70 32 32 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 732
Size: 94 638
MslI CAynn'nnrTG
Cuts at: 0 428 732
Size: 428 304
NspI r_CATG'y
Cuts at: 0 384 732
Size: 384 348
PasI CC'CwG_GG
Cuts at: 0 130 732
Size: 130 602
PfoI T'CCnGG_A
Cuts at: 0 282 732
Size: 282 450
PpuMI rG'GwC_Cy
Cuts at: 0 268 732
Size: 268 464
PspOMI G'GGCC_C
Cuts at: 0 70 152 732
Size: 70 82 580
Fragments arranged by size:
580 82 70
SapI GCTCTTCn'nnn_
Cuts at: 0 553 732
Size: 553 179
SmaI CCC'GGG
Cuts at: 0 258 732
Size: 258 474
SmlI C'TyrA_G
Cuts at: 0 65 732
Size: 65 667
StuI AGG'CCT
Cuts at: 0 589 732
Size: 589 143
StyI C'CwwG_G
Cuts at: 0 95 291 732
Size: 95 196 441
Fragments arranged by size:
441 196 95
TatI w'GTAC_w
Cuts at: 0 470 732
Size: 470 262
Tth111I GACn'n_nGTC
Cuts at: 0 628 732
Size: 628 104
XmaI C'CCGG_G
Cuts at: 0 256 732
Size: 256 476
Enzymes that do cut:
AccI AcuI ApaI ApoI AvaI BaeI BanI BanII
BclI BglI BglII BlpI Bme1580I BpmI BpuEI BsaWI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BstXI BstYI BstZ17I Bsu36I BtsI EarI Eco57MI EcoNI
EcoO109I Hin4I MmeI MslI NspI PasI PfoI PpuMI
PspOMI SapI SmaI SmlI StuI StyI TatI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI
BfrBI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BseRI BsiEI BsiWI BsmBI BspEI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI XbaI XcmI
XhoI XmnI ZraI