Restriction map for
pFN21AE1739
(Linear) (Six-base) MAPSORT of: KIEE1739.seq Check: 3802 from: 1 to: 732
KIEE1739.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 732
Size: 310 422
AleI CACnn'nnGTG
Cuts at: 0 610 732
Size: 610 122
AlwNI CAG_nnn'CTG
Cuts at: 0 219 732
Size: 219 513
AvaI C'yCGr_G
Cuts at: 0 181 253 523 535 732
Size: 181 72 270 12 197
Fragments arranged by size:
270 197 181 72 12
BanI G'GyrC_C
Cuts at: 0 105 432 502 541 732
Size: 105 327 70 39 191
Fragments arranged by size:
327 191 105 70 39
BanII G_rGCy'C
Cuts at: 0 260 732
Size: 260 472
BbeI G_GCGC'C
Cuts at: 0 436 506 545 732
Size: 436 70 39 187
Fragments arranged by size:
436 187 70 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 732
Size: 709 23
BglII A'GATC_T
Cuts at: 0 243 732
Size: 243 489
Bme1580I G_kGCm'C
Cuts at: 0 9 143 189 520 732
Size: 9 134 46 331 212
Fragments arranged by size:
331 212 134 46 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 214 732
Size: 19 195 518
Fragments arranged by size:
518 195 19
BmtI G_CTAG'C
Cuts at: 0 64 732
Size: 64 668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 37 542 732
Size: 22 15 505 190
Fragments arranged by size:
505 190 22 15
BsaHI Gr'CG_yC
Cuts at: 0 362 433 503 542 732
Size: 362 71 70 39 190
Fragments arranged by size:
362 190 71 70 39
BsaWI w'CCGG_w
Cuts at: 0 638 732
Size: 638 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 540 570 732
Size: 146 30 364 30 162
Fragments arranged by size:
364 162 146 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 241 244 732
Size: 241 3 488
Fragments arranged by size:
488 241 3
BseYI C'CCAG_C
Cuts at: 0 194 261 308 732
Size: 194 67 47 424
Fragments arranged by size:
424 194 67 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 732
Size: 652 80
BsiEI CG_ry'CG
Cuts at: 0 176 732
Size: 176 556
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 307 541 732
Size: 163 144 234 191
Fragments arranged by size:
234 191 163 144
Bsp1286I G_dGCh'C
Cuts at: 0 9 143 189 260 520 732
Size: 9 134 46 71 260 212
Fragments arranged by size:
260 212 134 71 46 9
BspEI T'CCGG_A
Cuts at: 0 638 732
Size: 638 94
BsrBI CCG'CTC
Cuts at: 0 344 464 732
Size: 344 120 268
Fragments arranged by size:
344 268 120
BssSI C'ACGA_G
Cuts at: 0 606 732
Size: 606 126
BstEII G'GTnAC_C
Cuts at: 0 87 732
Size: 87 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 732
Size: 84 648
BstYI r'GATC_y
Cuts at: 0 243 732
Size: 243 489
EaeI y'GGCC_r
Cuts at: 0 173 572 732
Size: 173 399 160
Fragments arranged by size:
399 173 160
EagI C'GGCC_G
Cuts at: 0 173 732
Size: 173 559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 37 310 542 732
Size: 22 15 273 232 190
Fragments arranged by size:
273 232 190 22 15
EcoO109I rG'GnC_Cy
Cuts at: 0 450 732
Size: 450 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 732
Size: 726 6
FspI TGC'GCA
Cuts at: 0 478 732
Size: 478 254
HaeII r_GCGC'y
Cuts at: 0 284 379 436 506 545 732
Size: 284 95 57 70 39 187
Fragments arranged by size:
284 187 95 70 57 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 540 572 732
Size: 540 32 160
Fragments arranged by size:
540 160 32
HincII GTy'rAC
Cuts at: 0 663 732
Size: 663 69
KasI G'GCGC_C
Cuts at: 0 432 502 541 732
Size: 432 70 39 191
Fragments arranged by size:
432 191 70 39
MslI CAynn'nnrTG
Cuts at: 0 610 732
Size: 610 122
MspA1I CmG'CkG
Cuts at: 0 265 288 732
Size: 265 23 444
Fragments arranged by size:
444 265 23
NarI GG'CG_CC
Cuts at: 0 433 503 542 732
Size: 433 70 39 190
Fragments arranged by size:
433 190 70 39
NheI G'CTAG_C
Cuts at: 0 60 732
Size: 60 672
NruI TCG'CGA
Cuts at: 0 694 732
Size: 694 38
PasI CC'CwG_GG
Cuts at: 0 456 732
Size: 456 276
PpuMI rG'GwC_Cy
Cuts at: 0 450 732
Size: 450 282
PspXI vC'TCGA_Gb
Cuts at: 0 535 732
Size: 535 197
PvuII CAG'CTG
Cuts at: 0 265 732
Size: 265 467
SanDI GG'GwC_CC
Cuts at: 0 450 732
Size: 450 282
SfoI GGC'GCC
Cuts at: 0 434 504 543 732
Size: 434 70 39 189
Fragments arranged by size:
434 189 70 39
SmaI CCC'GGG
Cuts at: 0 183 255 732
Size: 183 72 477
Fragments arranged by size:
477 183 72
SmlI C'TyrA_G
Cuts at: 0 535 732
Size: 535 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 456 732
Size: 456 276
XhoI C'TCGA_G
Cuts at: 0 535 732
Size: 535 197
XmaI C'CCGG_G
Cuts at: 0 181 253 732
Size: 181 72 479
Fragments arranged by size:
479 181 72
XmnI GAAnn'nnTTC
Cuts at: 0 706 732
Size: 706 26
Enzymes that do cut:
AcuI AleI AlwNI AvaI BanI BanII BbeI BbsI
BglII Bme1580I BmrI BmtI BpmI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BsrBI
BssSI BstEII BstXI BstYI EaeI EagI Eco57MI EcoO109I
FalI FspI HaeII Hin4I HincII KasI MslI MspA1I
NarI NheI NruI PasI PpuMI PspXI PvuII SanDI
SfoI SmaI SmlI TaqII XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsiHKAI BsiWI BsmI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NotI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XcmI ZraI