(Linear) (Six-base) MAPSORT of: KIEE1738.seq  Check: 43  from: 1  to: 732



KIEE1738.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     475     732 
   Size:         86     389     257

  Fragments arranged by size: 

                 389     257      86
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     620     652     732 
   Size:        620      32      80

  Fragments arranged by size: 

                 620      80      32
 
ApoI r'AATT_y

Cuts at:      0     194     496     732 
   Size:        194     302     236

  Fragments arranged by size: 

                 302     236     194
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     704     732 
   Size:        704      28
 
BclI T'GATC_A

Cuts at:      0     443     732 
   Size:        443     289
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     499     732 
   Size:        499     233
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     707     732 
   Size:        707      25
 
BseYI C'CCAG_C

Cuts at:      0     418     610     732 
   Size:        418     192     122

  Fragments arranged by size: 

                 418     192     122
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     283     732 
   Size:        283     449
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      31     403     732 
   Size:         31     372     329

  Fragments arranged by size: 

                 372     329      31
 
BtsI GCAGTG_nn'

Cuts at:      0     136     401     732 
   Size:        136     265     331

  Fragments arranged by size: 

                 331     265     136
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     522     557     732 
   Size:        522      35     175

  Fragments arranged by size: 

                 522     175      35
 
DraI TTT'AAA

Cuts at:      0     288     732 
   Size:        288     444
 
EarI CTCTTCn'nnn_

Cuts at:      0     454     732 
   Size:        454     278
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     475     732 
   Size:         86     389     257

  Fragments arranged by size: 

                 389     257      86
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     480     732 
   Size:        480     252
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      12      44     732 
   Size:         12      32     688

  Fragments arranged by size: 

                 688      32      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     674     732 
   Size:        674      58
 
PasI CC'CwG_GG

Cuts at:      0     703     732 
   Size:        703      29
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     732 
   Size:        160      32     540

  Fragments arranged by size: 

                 540     160      32
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     417     449     732 
   Size:        417      32     283

  Fragments arranged by size: 

                 417     283      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0     454     732 
   Size:        454     278
 
TatI w'GTAC_w

Cuts at:      0     243     732 
   Size:        243     489
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     143     516     732 
   Size:        143     373     216

  Fragments arranged by size: 

                 373     216     143
 

 Enzymes that do cut:

     AcuI     AloI     ApoI     BbsI     BclI     BsaI    BseRI    BseYI
    BspMI   BstAPI     BtsI    CspCI     DraI     EarI  Eco57MI     FalI
    Hin4I     MmeI     PasI     PpiI     PsrI     SapI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI