Restriction map for
pFN21AE1734
(Linear) (Six-base) MAPSORT of: KIEE1734.seq Check: 5122 from: 1 to: 723
KIEE1734.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AccI GT'mk_AC
Cuts at: 0 578 723
Size: 578 145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 723
Size: 145 578
AflIII A'CryG_T
Cuts at: 0 112 723
Size: 112 611
ApoI r'AATT_y
Cuts at: 0 433 445 723
Size: 433 12 278
Fragments arranged by size:
433 278 12
AvaI C'yCGr_G
Cuts at: 0 45 723
Size: 45 678
BanI G'GyrC_C
Cuts at: 0 213 366 723
Size: 213 153 357
Fragments arranged by size:
357 213 153
BanII G_rGCy'C
Cuts at: 0 67 683 722 723
Size: 67 616 39 1
Fragments arranged by size:
616 67 39 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 487 723
Size: 416 71 236
Fragments arranged by size:
416 236 71
BglI GCCn_nnn'nGGC
Cuts at: 0 48 723
Size: 48 675
BsaBI GATnn'nnATC
Cuts at: 0 73 723
Size: 73 650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 723
Size: 475 248
BsiHKAI G_wGCw'C
Cuts at: 0 683 723
Size: 683 40
Bsp1286I G_dGCh'C
Cuts at: 0 67 683 722 723
Size: 67 616 39 1
Fragments arranged by size:
616 67 39 1
BsrDI GCAATG_nn'
Cuts at: 0 563 723
Size: 563 160
BsrFI r'CCGG_y
Cuts at: 0 51 723
Size: 51 672
BssHII G'CGCG_C
Cuts at: 0 38 723
Size: 38 685
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 723
Size: 24 699
BstYI r'GATC_y
Cuts at: 0 624 723
Size: 624 99
Bsu36I CC'TnA_GG
Cuts at: 0 701 723
Size: 701 22
EcoICRI GAG'CTC
Cuts at: 0 681 723
Size: 681 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 723
Size: 145 578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 504 723
Size: 363 141 219
Fragments arranged by size:
363 219 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 723
Size: 99 32 592
Fragments arranged by size:
592 99 32
HindIII A'AGCT_T
Cuts at: 0 329 384 723
Size: 329 55 339
Fragments arranged by size:
339 329 55
MluI A'CGCG_T
Cuts at: 0 112 723
Size: 112 611
MspA1I CmG'CkG
Cuts at: 0 243 723
Size: 243 480
PasI CC'CwG_GG
Cuts at: 0 292 723
Size: 292 431
PfoI T'CCnGG_A
Cuts at: 0 56 723
Size: 56 667
PvuII CAG'CTG
Cuts at: 0 243 723
Size: 243 480
SacI G_AGCT'C
Cuts at: 0 683 723
Size: 683 40
SfcI C'TryA_G
Cuts at: 0 78 723
Size: 78 645
SmaI CCC'GGG
Cuts at: 0 47 723
Size: 47 676
SpeI A'CTAG_T
Cuts at: 0 649 723
Size: 649 74
StuI AGG'CCT
Cuts at: 0 706 723
Size: 706 17
StyI C'CwwG_G
Cuts at: 0 131 374 723
Size: 131 243 349
Fragments arranged by size:
349 243 131
XmaI C'CCGG_G
Cuts at: 0 45 723
Size: 45 678
XmnI GAAnn'nnTTC
Cuts at: 0 434 723
Size: 434 289
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI BanI BanII BbsI
BglI BsaBI BseRI BsiHKAI Bsp1286I BsrDI BsrFI BssHII
BstXI BstYI Bsu36I EcoICRI Eco57MI FalI Hin4I HindIII
MluI MspA1I PasI PfoI PvuII SacI SfcI SmaI
SpeI StuI StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SphI SrfI SspI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI ZraI