Restriction map for
pFN21AE1733
(Linear) (Six-base) MAPSORT of: KIEE1733.seq Check: 5740 from: 1 to: 720
KIEE1733.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 720
Size: 443 277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 446 720
Size: 446 274
AlwNI CAG_nnn'CTG
Cuts at: 0 441 720
Size: 441 279
AvaI C'yCGr_G
Cuts at: 0 509 720
Size: 509 211
AvrII C'CTAG_G
Cuts at: 0 523 720
Size: 523 197
BanII G_rGCy'C
Cuts at: 0 70 188 720
Size: 70 118 532
Fragments arranged by size:
532 118 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 602 636 720
Size: 602 34 84
Fragments arranged by size:
602 84 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 160 720
Size: 160 560
Bme1580I G_kGCm'C
Cuts at: 0 139 720
Size: 139 581
BmgBI CAC'GTC
Cuts at: 0 50 720
Size: 50 670
BmrI ACTGGGnnnn_n'
Cuts at: 0 292 720
Size: 292 428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 720
Size: 379 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 573 720
Size: 390 183 147
Fragments arranged by size:
390 183 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 273 303 720
Size: 273 30 417
Fragments arranged by size:
417 273 30
BseYI C'CCAG_C
Cuts at: 0 122 309 720
Size: 122 187 411
Fragments arranged by size:
411 187 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 720
Size: 679 41
BsmI GAATG_Cn'
Cuts at: 0 23 553 720
Size: 23 530 167
Fragments arranged by size:
530 167 23
Bsp1286I G_dGCh'C
Cuts at: 0 70 139 188 720
Size: 70 69 49 532
Fragments arranged by size:
532 70 69 49
BsrDI GCAATG_nn'
Cuts at: 0 196 720
Size: 196 524
BsrGI T'GTAC_A
Cuts at: 0 268 720
Size: 268 452
BstAPI GCAn_nnn'nTGC
Cuts at: 0 217 720
Size: 217 503
Bsu36I CC'TnA_GG
Cuts at: 0 569 720
Size: 569 151
DraIII CAC_nnn'GTG
Cuts at: 0 300 720
Size: 300 420
EaeI y'GGCC_r
Cuts at: 0 2 720
Size: 2 718
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 720
Size: 106 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 443 720
Size: 379 64 277
Fragments arranged by size:
379 277 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 358 720
Size: 358 362
EcoO109I rG'GnC_Cy
Cuts at: 0 575 600 635 720
Size: 575 25 35 85
Fragments arranged by size:
575 85 35 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 720
Size: 495 225
MslI CAynn'nnrTG
Cuts at: 0 384 428 720
Size: 384 44 292
Fragments arranged by size:
384 292 44
MspA1I CmG'CkG
Cuts at: 0 98 720
Size: 98 622
NspI r_CATG'y
Cuts at: 0 121 720
Size: 121 599
PfoI T'CCnGG_A
Cuts at: 0 356 720
Size: 356 364
PpuMI rG'GwC_Cy
Cuts at: 0 575 600 635 720
Size: 575 25 35 85
Fragments arranged by size:
575 85 35 25
PshAI GACnn'nnGTC
Cuts at: 0 98 720
Size: 98 622
PstI C_TGCA'G
Cuts at: 0 206 720
Size: 206 514
RsrII CG'GwC_CG
Cuts at: 0 93 720
Size: 93 627
SanDI GG'GwC_CC
Cuts at: 0 575 720
Size: 575 145
SfcI C'TryA_G
Cuts at: 0 202 720
Size: 202 518
SmaI CCC'GGG
Cuts at: 0 511 720
Size: 511 209
SmlI C'TyrA_G
Cuts at: 0 405 552 720
Size: 405 147 168
Fragments arranged by size:
405 168 147
SphI G_CATG'C
Cuts at: 0 121 720
Size: 121 599
StuI AGG'CCT
Cuts at: 0 275 720
Size: 275 445
StyI C'CwwG_G
Cuts at: 0 523 720
Size: 523 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 720
Size: 311 409
TatI w'GTAC_w
Cuts at: 0 268 720
Size: 268 452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 273 305 720
Size: 273 32 415
Fragments arranged by size:
415 273 32
Tth111I GACn'n_nGTC
Cuts at: 0 175 720
Size: 175 545
XmaI C'CCGG_G
Cuts at: 0 509 720
Size: 509 211
Enzymes that do cut:
AcuI AlfI AlwNI AvaI AvrII BanII BcgI BciVI
Bme1580I BmgBI BmrI BpmI BpuEI BsaXI BseYI BsgI
BsmI Bsp1286I BsrDI BsrGI BstAPI Bsu36I DraIII EaeI
EciI Eco57MI EcoNI EcoO109I MmeI MslI MspA1I NspI
PfoI PpuMI PshAI PstI RsrII SanDI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BanI BbeI BbsI BbvCI
BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XcmI
XhoI XmnI ZraI