Restriction map for
pFN21AE1725
(Linear) (Six-base) MAPSORT of: KIEE1725.seq Check: 3603 from: 1 to: 705
KIEE1725.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 648 705
Size: 648 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 705
Size: 442 263
AfeI AGC'GCT
Cuts at: 0 241 705
Size: 241 464
AlwNI CAG_nnn'CTG
Cuts at: 0 687 705
Size: 687 18
AvaI C'yCGr_G
Cuts at: 0 63 705
Size: 63 642
BanI G'GyrC_C
Cuts at: 0 12 455 608 660 705
Size: 12 443 153 52 45
Fragments arranged by size:
443 153 52 45 12
BanII G_rGCy'C
Cuts at: 0 28 64 206 705
Size: 28 36 142 499
Fragments arranged by size:
499 142 36 28
BbeI G_GCGC'C
Cuts at: 0 16 705
Size: 16 689
BbsI GAAGACnn'nnnn_
Cuts at: 0 349 705
Size: 349 356
BlpI GC'TnA_GC
Cuts at: 0 58 705
Size: 58 647
Bme1580I G_kGCm'C
Cuts at: 0 665 705
Size: 665 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 163 668 705
Size: 163 505 37
Fragments arranged by size:
505 163 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 313 481 541 705
Size: 42 271 168 60 164
Fragments arranged by size:
271 168 164 60 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 705
Size: 241 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 705
Size: 391 314
BsaHI Gr'CG_yC
Cuts at: 0 13 705
Size: 13 692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 705
Size: 454 30 221
Fragments arranged by size:
454 221 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 102 214 474 550 705
Size: 31 71 112 260 76 155
Fragments arranged by size:
260 155 112 76 71 31
BseYI C'CCAG_C
Cuts at: 0 268 349 705
Size: 268 81 356
Fragments arranged by size:
356 268 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 705
Size: 598 107
BsiHKAI G_wGCw'C
Cuts at: 0 64 206 704 705
Size: 64 142 498 1
Fragments arranged by size:
498 142 64 1
Bsp1286I G_dGCh'C
Cuts at: 0 28 64 206 665 704 705
Size: 28 36 142 459 39 1
Fragments arranged by size:
459 142 39 36 28 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 197 648 695 705
Size: 161 36 451 47 10
Fragments arranged by size:
451 161 47 36 10
BsrBI CCG'CTC
Cuts at: 0 252 705
Size: 252 453
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 705
Size: 288 417
BstXI CCAn_nnnn'nTGG
Cuts at: 0 503 705
Size: 503 202
BtgI C'CryG_G
Cuts at: 0 176 502 578 705
Size: 176 326 76 127
Fragments arranged by size:
326 176 127 76
BtsI GCAGTG_nn'
Cuts at: 0 475 705
Size: 475 230
DrdI GACnn_nn'nnGTC
Cuts at: 0 124 705
Size: 124 581
EaeI y'GGCC_r
Cuts at: 0 596 705
Size: 596 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 648 705
Size: 648 57
EcoICRI GAG'CTC
Cuts at: 0 62 204 705
Size: 62 142 501
Fragments arranged by size:
501 142 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 313 442 481 541 705
Size: 42 271 129 39 60 164
Fragments arranged by size:
271 164 129 60 42 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 705
Size: 391 314
EcoO109I rG'GnC_Cy
Cuts at: 0 77 213 684 705
Size: 77 136 471 21
Fragments arranged by size:
471 136 77 21
HaeII r_GCGC'y
Cuts at: 0 16 243 421 705
Size: 16 227 178 284
Fragments arranged by size:
284 227 178 16
KasI G'GCGC_C
Cuts at: 0 12 705
Size: 12 693
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 140 209 705
Size: 120 20 69 496
Fragments arranged by size:
496 120 69 20
MslI CAynn'nnrTG
Cuts at: 0 501 705
Size: 501 204
MspA1I CmG'CkG
Cuts at: 0 353 411 601 705
Size: 353 58 190 104
Fragments arranged by size:
353 190 104 58
NarI GG'CG_CC
Cuts at: 0 13 705
Size: 13 692
NcoI C'CATG_G
Cuts at: 0 502 705
Size: 502 203
PasI CC'CwG_GG
Cuts at: 0 649 705
Size: 649 56
PpuMI rG'GwC_Cy
Cuts at: 0 77 684 705
Size: 77 607 21
Fragments arranged by size:
607 77 21
PstI C_TGCA'G
Cuts at: 0 194 395 470 705
Size: 194 201 75 235
Fragments arranged by size:
235 201 194 75
PvuII CAG'CTG
Cuts at: 0 353 411 705
Size: 353 58 294
Fragments arranged by size:
353 294 58
SacI G_AGCT'C
Cuts at: 0 64 206 705
Size: 64 142 499
Fragments arranged by size:
499 142 64
SanDI GG'GwC_CC
Cuts at: 0 77 705
Size: 77 628
SbfI CC_TGCA'GG
Cuts at: 0 194 395 705
Size: 194 201 310
Fragments arranged by size:
310 201 194
SfcI C'TryA_G
Cuts at: 0 190 391 466 705
Size: 190 201 75 239
Fragments arranged by size:
239 201 190 75
SfoI GGC'GCC
Cuts at: 0 14 705
Size: 14 691
SmlI C'TyrA_G
Cuts at: 0 256 705
Size: 256 449
StyI C'CwwG_G
Cuts at: 0 502 705
Size: 502 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 705
Size: 454 32 219
Fragments arranged by size:
454 219 32
Enzymes that do cut:
AarI AcuI AfeI AlwNI AvaI BanI BanII BbeI
BbsI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BstAPI BstXI BtgI BtsI DrdI EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KasI MmeI MslI MspA1I
NarI NcoI PasI PpuMI PstI PvuII SacI SanDI
SbfI SfcI SfoI SmlI StyI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EarI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI