Restriction map for
pFN21AE1723
(Linear) (Six-base) MAPSORT of: KIEE1723.seq Check: 6655 from: 1 to: 702
KIEE1723.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AflIII A'CryG_T
Cuts at: 0 114 702
Size: 114 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 702
Size: 31 671
AlwNI CAG_nnn'CTG
Cuts at: 0 194 681 702
Size: 194 487 21
Fragments arranged by size:
487 194 21
AvaI C'yCGr_G
Cuts at: 0 50 73 702
Size: 50 23 629
Fragments arranged by size:
629 50 23
BanI G'GyrC_C
Cuts at: 0 417 702
Size: 417 285
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 702
Size: 355 347
BclI T'GATC_A
Cuts at: 0 155 702
Size: 155 547
BglI GCCn_nnn'nGGC
Cuts at: 0 259 594 702
Size: 259 335 108
Fragments arranged by size:
335 259 108
BmgBI CAC'GTC
Cuts at: 0 249 702
Size: 249 453
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 702
Size: 613 89
BsaWI w'CCGG_w
Cuts at: 0 206 702
Size: 206 496
BseYI C'CCAG_C
Cuts at: 0 40 702
Size: 40 662
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 350 702
Size: 180 170 352
Fragments arranged by size:
352 180 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 105 239 702
Size: 105 134 463
Fragments arranged by size:
463 134 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 702
Size: 23 679
BsrDI GCAATG_nn'
Cuts at: 0 691 702
Size: 691 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 194 464 681 702
Size: 194 270 217 21
Fragments arranged by size:
270 217 194 21
BstYI r'GATC_y
Cuts at: 0 59 702
Size: 59 643
Bsu36I CC'TnA_GG
Cuts at: 0 579 702
Size: 579 123
BtgI C'CryG_G
Cuts at: 0 215 332 644 702
Size: 215 117 312 58
Fragments arranged by size:
312 215 117 58
DraIII CAC_nnn'GTG
Cuts at: 0 549 702
Size: 549 153
DrdI GACnn_nn'nnGTC
Cuts at: 0 119 702
Size: 119 583
EaeI y'GGCC_r
Cuts at: 0 335 425 702
Size: 335 90 277
Fragments arranged by size:
335 277 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 384 702
Size: 384 318
EcoO109I rG'GnC_Cy
Cuts at: 0 261 377 702
Size: 261 116 325
Fragments arranged by size:
325 261 116
HaeII r_GCGC'y
Cuts at: 0 226 702
Size: 226 476
MluI A'CGCG_T
Cuts at: 0 114 702
Size: 114 588
MscI TGG'CCA
Cuts at: 0 337 702
Size: 337 365
MspA1I CmG'CkG
Cuts at: 0 423 702
Size: 423 279
NcoI C'CATG_G
Cuts at: 0 215 332 702
Size: 215 117 370
Fragments arranged by size:
370 215 117
PasI CC'CwG_GG
Cuts at: 0 264 702
Size: 264 438
PfoI T'CCnGG_A
Cuts at: 0 470 702
Size: 470 232
PpuMI rG'GwC_Cy
Cuts at: 0 377 702
Size: 377 325
SexAI A'CCwGG_T
Cuts at: 0 413 702
Size: 413 289
SfcI C'TryA_G
Cuts at: 0 180 702
Size: 180 522
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 259 702
Size: 259 443
SmaI CCC'GGG
Cuts at: 0 52 702
Size: 52 650
StuI AGG'CCT
Cuts at: 0 577 702
Size: 577 125
StyI C'CwwG_G
Cuts at: 0 215 332 702
Size: 215 117 370
Fragments arranged by size:
370 215 117
TatI w'GTAC_w
Cuts at: 0 140 702
Size: 140 562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 702
Size: 411 291
XmaI C'CCGG_G
Cuts at: 0 50 702
Size: 50 652
Enzymes that do cut:
AflIII AlfI AlwNI AvaI BanI BbsI BclI BglI
BmgBI BmrI BsaWI BseYI BsgI BsmBI BspMI BsrDI
BstAPI BstYI Bsu36I BtgI DraIII DrdI EaeI EcoNI
EcoO109I HaeII MluI MscI MspA1I NcoI PasI PfoI
PpuMI SexAI SfcI SfiI SmaI StuI StyI TatI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BfrBI BglII BlpI Bme1580I BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI ZraI