Restriction map for
pFN21AE1720
(Linear) (Six-base) MAPSORT of: KIEE1720.seq Check: 5326 from: 1 to: 681
KIEE1720.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 451 587 681
Size: 388 63 136 94
Fragments arranged by size:
388 136 94 63
AvaI C'yCGr_G
Cuts at: 0 243 512 681
Size: 243 269 169
Fragments arranged by size:
269 243 169
BamHI G'GATC_C
Cuts at: 0 285 681
Size: 285 396
BanI G'GyrC_C
Cuts at: 0 358 681
Size: 358 323
BanII G_rGCy'C
Cuts at: 0 647 681
Size: 647 34
BbeI G_GCGC'C
Cuts at: 0 362 681
Size: 362 319
BglI GCCn_nnn'nGGC
Cuts at: 0 97 409 681
Size: 97 312 272
Fragments arranged by size:
312 272 97
Bme1580I G_kGCm'C
Cuts at: 0 93 681
Size: 93 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 49 573 681
Size: 49 524 108
Fragments arranged by size:
524 108 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 348 601 681
Size: 83 265 253 80
Fragments arranged by size:
265 253 83 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 681
Size: 49 632
BsaHI Gr'CG_yC
Cuts at: 0 359 681
Size: 359 322
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 599 629 681
Size: 599 30 52
Fragments arranged by size:
599 52 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 59 681
Size: 59 622
Bsp1286I G_dGCh'C
Cuts at: 0 93 647 681
Size: 93 554 34
Fragments arranged by size:
554 93 34
BspHI T'CATG_A
Cuts at: 0 47 584 681
Size: 47 537 97
Fragments arranged by size:
537 97 47
BsrFI r'CCGG_y
Cuts at: 0 423 681
Size: 423 258
BstYI r'GATC_y
Cuts at: 0 285 681
Size: 285 396
BtgI C'CryG_G
Cuts at: 0 251 681
Size: 251 430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 427 681
Size: 427 254
DraIII CAC_nnn'GTG
Cuts at: 0 433 681
Size: 433 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 679 681
Size: 679 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 348 388 451 587 601 681
Size: 83 265 40 63 136 14 80
Fragments arranged by size:
265 136 83 80 63 40 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 681
Size: 496 185
EcoO109I rG'GnC_Cy
Cuts at: 0 99 132 681
Size: 99 33 549
Fragments arranged by size:
549 99 33
EcoRV GAT'ATC
Cuts at: 0 351 681
Size: 351 330
HaeII r_GCGC'y
Cuts at: 0 362 681
Size: 362 319
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 270 302 681
Size: 194 32 44 32 379
Fragments arranged by size:
379 194 44 32 32
KasI G'GCGC_C
Cuts at: 0 358 681
Size: 358 323
MslI CAynn'nnrTG
Cuts at: 0 52 681
Size: 52 629
NarI GG'CG_CC
Cuts at: 0 359 681
Size: 359 322
PfoI T'CCnGG_A
Cuts at: 0 217 681
Size: 217 464
PspXI vC'TCGA_Gb
Cuts at: 0 512 681
Size: 512 169
SfcI C'TryA_G
Cuts at: 0 198 681
Size: 198 483
SfoI GGC'GCC
Cuts at: 0 360 681
Size: 360 321
SmaI CCC'GGG
Cuts at: 0 245 681
Size: 245 436
SmlI C'TyrA_G
Cuts at: 0 512 681
Size: 512 169
StyI C'CwwG_G
Cuts at: 0 497 681
Size: 497 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 681
Size: 592 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 599 631 681
Size: 599 32 50
Fragments arranged by size:
599 50 32
XhoI C'TCGA_G
Cuts at: 0 512 681
Size: 512 169
XmaI C'CCGG_G
Cuts at: 0 243 681
Size: 243 438
XmnI GAAnn'nnTTC
Cuts at: 0 547 681
Size: 547 134
Enzymes that do cut:
AcuI AvaI BamHI BanI BanII BbeI BglI Bme1580I
BmrI BpmI BsaI BsaHI BsaXI BsmBI Bsp1286I BspHI
BsrFI BstYI BtgI BtgZI DraIII EciI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I KasI MslI NarI PfoI
PspXI SfcI SfoI SmaI SmlI StyI TaqII TstI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EcoICRI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
ZraI