Restriction map for
pFN21AE1719
(Linear) (Six-base) MAPSORT of: KIEE1719.seq Check: 5100 from: 1 to: 675
KIEE1719.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
Acc65I G'GTAC_C
Cuts at: 0 512 675
Size: 512 163
AvaI C'yCGr_G
Cuts at: 0 52 643 675
Size: 52 591 32
Fragments arranged by size:
591 52 32
BanI G'GyrC_C
Cuts at: 0 100 277 512 675
Size: 100 177 235 163
Fragments arranged by size:
235 177 163 100
BanII G_rGCy'C
Cuts at: 0 53 675
Size: 53 622
BbsI GAAGACnn'nnnn_
Cuts at: 0 571 675
Size: 571 104
BbvCI CC'TCA_GC
Cuts at: 0 197 386 675
Size: 197 189 289
Fragments arranged by size:
289 197 189
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 675
Size: 282 393
BglI GCCn_nnn'nGGC
Cuts at: 0 174 675
Size: 174 501
Bme1580I G_kGCm'C
Cuts at: 0 280 675
Size: 280 395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 622 675
Size: 232 390 53
Fragments arranged by size:
390 232 53
Bpu10I CC'TnA_GC
Cuts at: 0 197 386 675
Size: 197 189 289
Fragments arranged by size:
289 197 189
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 675
Size: 118 557
BseYI C'CCAG_C
Cuts at: 0 360 675
Size: 360 315
BsiEI CG_ry'CG
Cuts at: 0 67 178 675
Size: 67 111 497
Fragments arranged by size:
497 111 67
BsmI GAATG_Cn'
Cuts at: 0 387 675
Size: 387 288
Bsp1286I G_dGCh'C
Cuts at: 0 53 280 675
Size: 53 227 395
Fragments arranged by size:
395 227 53
BspHI T'CATG_A
Cuts at: 0 542 675
Size: 542 133
BsrFI r'CCGG_y
Cuts at: 0 62 66 675
Size: 62 4 609
Fragments arranged by size:
609 62 4
BstEII G'GTnAC_C
Cuts at: 0 339 675
Size: 339 336
BstXI CCAn_nnnn'nTGG
Cuts at: 0 600 675
Size: 600 75
BtgI C'CryG_G
Cuts at: 0 96 183 675
Size: 96 87 492
Fragments arranged by size:
492 96 87
EaeI y'GGCC_r
Cuts at: 0 11 64 175 374 501 608 675
Size: 11 53 111 199 127 107 67
Fragments arranged by size:
199 127 111 107 67 53 11
EagI C'GGCC_G
Cuts at: 0 64 175 675
Size: 64 111 500
Fragments arranged by size:
500 111 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 622 675
Size: 232 390 53
Fragments arranged by size:
390 232 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 675
Size: 526 149
HaeII r_GCGC'y
Cuts at: 0 10 356 675
Size: 10 346 319
Fragments arranged by size:
346 319 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 675
Size: 280 32 363
Fragments arranged by size:
363 280 32
KpnI G_GTAC'C
Cuts at: 0 516 675
Size: 516 159
MscI TGG'CCA
Cuts at: 0 13 376 610 675
Size: 13 363 234 65
Fragments arranged by size:
363 234 65 13
MslI CAynn'nnrTG
Cuts at: 0 376 675
Size: 376 299
MspA1I CmG'CkG
Cuts at: 0 81 98 185 421 577 675
Size: 81 17 87 236 156 98
Fragments arranged by size:
236 156 98 87 81 17
NaeI GCC'GGC
Cuts at: 0 64 675
Size: 64 611
NgoMIV G'CCGG_C
Cuts at: 0 62 675
Size: 62 613
PfoI T'CCnGG_A
Cuts at: 0 599 675
Size: 599 76
PvuII CAG'CTG
Cuts at: 0 421 577 675
Size: 421 156 98
Fragments arranged by size:
421 156 98
RsrII CG'GwC_CG
Cuts at: 0 180 675
Size: 180 495
SacII CC_GC'GG
Cuts at: 0 99 186 675
Size: 99 87 489
Fragments arranged by size:
489 99 87
SfcI C'TryA_G
Cuts at: 0 399 675
Size: 399 276
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 174 675
Size: 174 501
SmaI CCC'GGG
Cuts at: 0 54 675
Size: 54 621
StuI AGG'CCT
Cuts at: 0 649 675
Size: 649 26
StyI C'CwwG_G
Cuts at: 0 143 569 675
Size: 143 426 106
Fragments arranged by size:
426 143 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 675
Size: 319 356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 496 675
Size: 496 179
XmaI C'CCGG_G
Cuts at: 0 52 675
Size: 52 623
Enzymes that do cut:
Acc65I AvaI BanI BanII BbsI BbvCI BciVI BglI
Bme1580I BpmI Bpu10I BseRI BseYI BsiEI BsmI Bsp1286I
BspHI BsrFI BstEII BstXI BtgI EaeI EagI Eco57MI
EcoNI HaeII Hin4I KpnI MscI MslI MspA1I NaeI
NgoMIV PfoI PvuII RsrII SacII SfcI SfiI SmaI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MmeI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI