Restriction map for
pFN21AE1717
(Linear) (Six-base) MAPSORT of: KIEE1717.seq Check: 1889 from: 1 to: 669
KIEE1717.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AccI GT'mk_AC
Cuts at: 0 269 669
Size: 269 400
AlwNI CAG_nnn'CTG
Cuts at: 0 435 669
Size: 435 234
BamHI G'GATC_C
Cuts at: 0 398 669
Size: 398 271
BanII G_rGCy'C
Cuts at: 0 356 669
Size: 356 313
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 669
Size: 26 643
BbvCI CC'TCA_GC
Cuts at: 0 595 669
Size: 595 74
BglI GCCn_nnn'nGGC
Cuts at: 0 479 669
Size: 479 190
BglII A'GATC_T
Cuts at: 0 638 669
Size: 638 31
Bme1580I G_kGCm'C
Cuts at: 0 195 669
Size: 195 474
BmgBI CAC'GTC
Cuts at: 0 390 534 564 669
Size: 390 144 30 105
Fragments arranged by size:
390 144 105 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 669
Size: 396 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 340 669
Size: 96 244 329
Fragments arranged by size:
329 244 96
Bpu10I CC'TnA_GC
Cuts at: 0 595 669
Size: 595 74
BsaBI GATnn'nnATC
Cuts at: 0 397 669
Size: 397 272
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 625 669
Size: 331 294 44
Fragments arranged by size:
331 294 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 669
Size: 608 61
Bsp1286I G_dGCh'C
Cuts at: 0 195 356 669
Size: 195 161 313
Fragments arranged by size:
313 195 161
BstAPI GCAn_nnn'nTGC
Cuts at: 0 548 669
Size: 548 121
BstYI r'GATC_y
Cuts at: 0 398 638 669
Size: 398 240 31
Fragments arranged by size:
398 240 31
BtgI C'CryG_G
Cuts at: 0 74 669
Size: 74 595
BtsI GCAGTG_nn'
Cuts at: 0 591 669
Size: 591 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 183 669
Size: 183 486
EaeI y'GGCC_r
Cuts at: 0 102 437 605 669
Size: 102 335 168 64
Fragments arranged by size:
335 168 102 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 460 669
Size: 460 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 340 669
Size: 96 244 329
Fragments arranged by size:
329 244 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 607 669
Size: 313 294 62
Fragments arranged by size:
313 294 62
EcoO109I rG'GnC_Cy
Cuts at: 0 555 669
Size: 555 114
HaeII r_GCGC'y
Cuts at: 0 456 669
Size: 456 213
HincII GTy'rAC
Cuts at: 0 270 669
Size: 270 399
MscI TGG'CCA
Cuts at: 0 104 669
Size: 104 565
MspA1I CmG'CkG
Cuts at: 0 246 669
Size: 246 423
NspI r_CATG'y
Cuts at: 0 90 669
Size: 90 579
PfoI T'CCnGG_A
Cuts at: 0 133 356 669
Size: 133 223 313
Fragments arranged by size:
313 223 133
PpuMI rG'GwC_Cy
Cuts at: 0 555 669
Size: 555 114
PstI C_TGCA'G
Cuts at: 0 149 586 669
Size: 149 437 83
Fragments arranged by size:
437 149 83
SalI G'TCGA_C
Cuts at: 0 268 669
Size: 268 401
SfcI C'TryA_G
Cuts at: 0 145 582 651 669
Size: 145 437 69 18
Fragments arranged by size:
437 145 69 18
SphI G_CATG'C
Cuts at: 0 90 669
Size: 90 579
StuI AGG'CCT
Cuts at: 0 593 669
Size: 593 76
StyI C'CwwG_G
Cuts at: 0 41 669
Size: 41 628
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 396 669
Size: 87 309 273
Fragments arranged by size:
309 273 87
Tth111I GACn'n_nGTC
Cuts at: 0 565 669
Size: 565 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 669
Size: 306 363
XmnI GAAnn'nnTTC
Cuts at: 0 31 340 669
Size: 31 309 329
Fragments arranged by size:
329 309 31
Enzymes that do cut:
AccI AlwNI BamHI BanII BbsI BbvCI BglI BglII
Bme1580I BmgBI BplI BpmI Bpu10I BsaBI BseRI BsgI
Bsp1286I BstAPI BstYI BtgI BtsI DrdI EaeI EciI
Eco57MI EcoNI EcoO109I HaeII HincII MscI MspA1I NspI
PfoI PpuMI PstI SalI SfcI SphI StuI StyI
TaqII Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BcgI BciVI BclI BfrBI BlpI BmrI BmtI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EarI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TatI TstI XbaI XhoI XmaI ZraI