Restriction map for
pFN21AE1713
(Linear) (Six-base) MAPSORT of: KIEE1713.seq Check: 645 from: 1 to: 648
KIEE1713.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
Acc65I G'GTAC_C
Cuts at: 0 296 648
Size: 296 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 648
Size: 322 326
AflIII A'CryG_T
Cuts at: 0 557 648
Size: 557 91
AlwNI CAG_nnn'CTG
Cuts at: 0 333 648
Size: 333 315
ApaI G_GGCC'C
Cuts at: 0 95 648
Size: 95 553
ApaLI G'TGCA_C
Cuts at: 0 100 283 648
Size: 100 183 365
Fragments arranged by size:
365 183 100
BamHI G'GATC_C
Cuts at: 0 32 648
Size: 32 616
BanI G'GyrC_C
Cuts at: 0 65 134 296 310 648
Size: 65 69 162 14 338
Fragments arranged by size:
338 162 69 65 14
BanII G_rGCy'C
Cuts at: 0 95 164 648
Size: 95 69 484
Fragments arranged by size:
484 95 69
BbeI G_GCGC'C
Cuts at: 0 69 314 648
Size: 69 245 334
Fragments arranged by size:
334 245 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 648
Size: 359 289
BglI GCCn_nnn'nGGC
Cuts at: 0 386 648
Size: 386 262
Bme1580I G_kGCm'C
Cuts at: 0 95 104 287 648
Size: 95 9 183 361
Fragments arranged by size:
361 183 95 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 259 595 637 648
Size: 150 109 336 42 11
Fragments arranged by size:
336 150 109 42 11
Bpu10I CC'TnA_GC
Cuts at: 0 433 648
Size: 433 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 432 571 648
Size: 432 139 77
Fragments arranged by size:
432 139 77
BsaAI yAC'GTr
Cuts at: 0 27 99 648
Size: 27 72 549
Fragments arranged by size:
549 72 27
BsaHI Gr'CG_yC
Cuts at: 0 66 197 311 648
Size: 66 131 114 337
Fragments arranged by size:
337 131 114 66
BsaWI w'CCGG_w
Cuts at: 0 610 648
Size: 610 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 148 178 648
Size: 27 121 30 470
Fragments arranged by size:
470 121 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 385 535 642 648
Size: 382 3 150 107 6
Fragments arranged by size:
382 150 107 6 3
BseYI C'CCAG_C
Cuts at: 0 110 634 648
Size: 110 524 14
Fragments arranged by size:
524 110 14
BsiHKAI G_wGCw'C
Cuts at: 0 104 287 648
Size: 104 183 361
Fragments arranged by size:
361 183 104
Bsp1286I G_dGCh'C
Cuts at: 0 95 104 164 287 648
Size: 95 9 60 123 361
Fragments arranged by size:
361 123 95 60 9
BsrBI CCG'CTC
Cuts at: 0 8 648
Size: 8 640
BsrFI r'CCGG_y
Cuts at: 0 40 648
Size: 40 608
BsrGI T'GTAC_A
Cuts at: 0 140 458 648
Size: 140 318 190
Fragments arranged by size:
318 190 140
BssHII G'CGCG_C
Cuts at: 0 209 224 648
Size: 209 15 424
Fragments arranged by size:
424 209 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 291 333 648
Size: 234 57 42 315
Fragments arranged by size:
315 234 57 42
BstYI r'GATC_y
Cuts at: 0 32 374 648
Size: 32 342 274
Fragments arranged by size:
342 274 32
Bsu36I CC'TnA_GG
Cuts at: 0 527 535 648
Size: 527 8 113
Fragments arranged by size:
527 113 8
BtgI C'CryG_G
Cuts at: 0 56 156 266 509 648
Size: 56 100 110 243 139
Fragments arranged by size:
243 139 110 100 56
BtsI GCAGTG_nn'
Cuts at: 0 444 648
Size: 444 204
DraIII CAC_nnn'GTG
Cuts at: 0 291 648
Size: 291 357
EaeI y'GGCC_r
Cuts at: 0 38 53 59 269 648
Size: 38 15 6 210 379
Fragments arranged by size:
379 210 38 15 6
EarI CTCTTCn'nnn_
Cuts at: 0 570 648
Size: 570 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 367 648
Size: 115 252 281
Fragments arranged by size:
281 252 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 259 322 595 637 648
Size: 150 109 63 273 42 11
Fragments arranged by size:
273 150 109 63 42 11
EcoO109I rG'GnC_Cy
Cuts at: 0 91 92 531 648
Size: 91 1 439 117
Fragments arranged by size:
439 117 91 1
FseI GG_CCGG'CC
Cuts at: 0 44 648
Size: 44 604
FspI TGC'GCA
Cuts at: 0 181 648
Size: 181 467
HaeII r_GCGC'y
Cuts at: 0 69 314 648
Size: 69 245 334
Fragments arranged by size:
334 245 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 648
Size: 547 32 69
Fragments arranged by size:
547 69 32
KasI G'GCGC_C
Cuts at: 0 65 310 648
Size: 65 245 338
Fragments arranged by size:
338 245 65
KpnI G_GTAC'C
Cuts at: 0 300 648
Size: 300 348
MscI TGG'CCA
Cuts at: 0 271 648
Size: 271 377
MspA1I CmG'CkG
Cuts at: 0 170 450 456 648
Size: 170 280 6 192
Fragments arranged by size:
280 192 170 6
NaeI GCC'GGC
Cuts at: 0 42 648
Size: 42 606
NarI GG'CG_CC
Cuts at: 0 66 311 648
Size: 66 245 337
Fragments arranged by size:
337 245 66
NcoI C'CATG_G
Cuts at: 0 509 648
Size: 509 139
NgoMIV G'CCGG_C
Cuts at: 0 40 648
Size: 40 608
NspI r_CATG'y
Cuts at: 0 561 648
Size: 561 87
PciI A'CATG_T
Cuts at: 0 557 648
Size: 557 91
PmlI CAC'GTG
Cuts at: 0 99 648
Size: 99 549
PpuMI rG'GwC_Cy
Cuts at: 0 531 648
Size: 531 117
PspOMI G'GGCC_C
Cuts at: 0 91 648
Size: 91 557
PvuII CAG'CTG
Cuts at: 0 170 456 648
Size: 170 286 192
Fragments arranged by size:
286 192 170
RsrII CG'GwC_CG
Cuts at: 0 263 648
Size: 263 385
ScaI AGT'ACT
Cuts at: 0 343 648
Size: 343 305
SfoI GGC'GCC
Cuts at: 0 67 312 648
Size: 67 245 336
Fragments arranged by size:
336 245 67
StyI C'CwwG_G
Cuts at: 0 509 648
Size: 509 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 648
Size: 595 53
TatI w'GTAC_w
Cuts at: 0 140 341 458 648
Size: 140 201 117 190
Fragments arranged by size:
201 190 140 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 148 180 648
Size: 29 119 32 468
Fragments arranged by size:
468 119 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 196 648
Size: 196 452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 507 648
Size: 507 141
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaI ApaLI BamHI BanI
BanII BbeI BbsI BglI Bme1580I BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BsrBI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI
BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I FseI
FspI HaeII Hin4I KasI KpnI MscI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmlI PpuMI PspOMI
PvuII RsrII ScaI SfoI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FspAI HincII HindIII HpaI MfeI MluI MmeI MslI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmaI
XmnI ZraI