(Linear) (Six-base) MAPSORT of: KIEE1710.seq  Check: 740  from: 1  to: 645



KIEE1710.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

Acc65I G'GTAC_C

Cuts at:      0     301     645 
   Size:        301     344
 
AclI AA'CG_TT

Cuts at:      0     283     645 
   Size:        283     362
 
AvaI C'yCGr_G

Cuts at:      0     312     645 
   Size:        312     333
 
BanI G'GyrC_C

Cuts at:      0     301     364     630     645 
   Size:        301      63     266      15

  Fragments arranged by size: 

                 301     266      63      15
 
BbeI G_GCGC'C

Cuts at:      0     368     645 
   Size:        368     277
 
BglI GCCn_nnn'nGGC

Cuts at:      0     430     645 
   Size:        430     215
 
BmgBI CAC'GTC

Cuts at:      0     225     645 
   Size:        225     420
 
BmtI G_CTAG'C

Cuts at:      0     437     645 
   Size:        437     208
 
Bpu10I CC'TnA_GC

Cuts at:      0      83     560     645 
   Size:         83     477      85

  Fragments arranged by size: 

                 477      85      83
 
BsaHI Gr'CG_yC

Cuts at:      0     340     365     645 
   Size:        340      25     280

  Fragments arranged by size: 

                 340     280      25
 
BsaWI w'CCGG_w

Cuts at:      0     304     645 
   Size:        304     341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      30     645 
   Size:         30     615
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     147     645 
   Size:        147     498
 
BseYI C'CCAG_C

Cuts at:      0     252     645 
   Size:        252     393
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     310     645 
   Size:        163     147     335

  Fragments arranged by size: 

                 335     163     147
 
BsiEI CG_ry'CG

Cuts at:      0     212     645 
   Size:        212     433
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      87     645 
   Size:         87     558
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     400     645 
   Size:        400     245
 
BtgI C'CryG_G

Cuts at:      0     347     425     645 
   Size:        347      78     220

  Fragments arranged by size: 

                 347     220      78
 
EaeI y'GGCC_r

Cuts at:      0     209     581     645 
   Size:        209     372      64

  Fragments arranged by size: 

                 372     209      64
 
EagI C'GGCC_G

Cuts at:      0     209     645 
   Size:        209     436
 
HaeII r_GCGC'y

Cuts at:      0     205     368     645 
   Size:        205     163     277

  Fragments arranged by size: 

                 277     205     163
 
KasI G'GCGC_C

Cuts at:      0     364     645 
   Size:        364     281
 
KpnI G_GTAC'C

Cuts at:      0     305     645 
   Size:        305     340
 
MspA1I CmG'CkG

Cuts at:      0     115     645 
   Size:        115     530
 
NarI GG'CG_CC

Cuts at:      0     365     645 
   Size:        365     280
 
NheI G'CTAG_C

Cuts at:      0     433     645 
   Size:        433     212
 
NotI GC'GGCC_GC

Cuts at:      0     209     645 
   Size:        209     436
 
PstI C_TGCA'G

Cuts at:      0      93     645 
   Size:         93     552
 
SfcI C'TryA_G

Cuts at:      0      89     645 
   Size:         89     556
 
SfoI GGC'GCC

Cuts at:      0     366     645 
   Size:        366     279
 
StyI C'CwwG_G

Cuts at:      0     575     584     645 
   Size:        575       9      61

  Fragments arranged by size: 

                 575      61       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     272     304     645 
   Size:        272      32     341

  Fragments arranged by size: 

                 341     272      32
 

 Enzymes that do cut:

   Acc65I     AclI     AvaI     BanI     BbeI     BglI    BmgBI     BmtI
   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BspMI   BstAPI     BtgI     EaeI     EagI    HaeII     KasI     KpnI
   MspA1I     NarI     NheI     NotI     PstI     SfcI     SfoI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI