(Linear) (Six-base) MAPSORT of: KIEE1705.seq  Check: 5181  from: 1  to: 627



KIEE1705.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AflII C'TTAA_G

Cuts at:      0      16     627 
   Size:         16     611
 
AflIII A'CryG_T

Cuts at:      0     489     627 
   Size:        489     138
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     257     627 
   Size:        257     370
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      95     627 
   Size:         95     532
 
BglII A'GATC_T

Cuts at:      0     168     627 
   Size:        168     459
 
BsmI GAATG_Cn'

Cuts at:      0     282     627 
   Size:        282     345
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     563     627 
   Size:        563      64
 
BsrDI GCAATG_nn'

Cuts at:      0     101     215     627 
   Size:        101     114     412

  Fragments arranged by size: 

                 412     114     101
 
BstYI r'GATC_y

Cuts at:      0     168     627 
   Size:        168     459
 
EarI CTCTTCn'nnn_

Cuts at:      0     278     627 
   Size:        278     349
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      31     627 
   Size:         31     596
 
HincII GTy'rAC

Cuts at:      0     296     627 
   Size:        296     331
 
HpaI GTT'AAC

Cuts at:      0     296     627 
   Size:        296     331
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     627 
   Size:        213     414
 
MslI CAynn'nnrTG

Cuts at:      0     601     627 
   Size:        601      26
 
NspI r_CATG'y

Cuts at:      0     113     493     627 
   Size:        113     380     134

  Fragments arranged by size: 

                 380     134     113
 
PciI A'CATG_T

Cuts at:      0     489     627 
   Size:        489     138
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     476     508     627 
   Size:        476      32     119

  Fragments arranged by size: 

                 476     119      32
 
PstI C_TGCA'G

Cuts at:      0     510     627 
   Size:        510     117
 
ScaI AGT'ACT

Cuts at:      0     191     627 
   Size:        191     436
 
SfcI C'TryA_G

Cuts at:      0     506     627 
   Size:        506     121
 
SmlI C'TyrA_G

Cuts at:      0      16     627 
   Size:         16     611
 
SspI AAT'ATT

Cuts at:      0     266     303     627 
   Size:        266      37     324

  Fragments arranged by size: 

                 324     266      37
 
TatI w'GTAC_w

Cuts at:      0     189     627 
   Size:        189     438
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      72     627 
   Size:         72     555
 
XmnI GAAnn'nnTTC

Cuts at:      0     491     627 
   Size:        491     136
 

 Enzymes that do cut:

    AflII   AflIII     BbsI    BciVI    BglII     BsmI    BspMI    BsrDI
    BstYI     EarI     FalI   HincII     HpaI     MmeI     MslI     NspI
     PciI     PpiI     PstI     ScaI     SfcI     SmlI     SspI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI