(Linear) (Six-base) MAPSORT of: KIEE1703.seq  Check: 8199  from: 1  to: 624



KIEE1703.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

Acc65I G'GTAC_C

Cuts at:      0      48     624 
   Size:         48     576
 
AhdI GACnn_n'nnGTC

Cuts at:      0     148     624 
   Size:        148     476
 
ApoI r'AATT_y

Cuts at:      0     286     547     624 
   Size:        286     261      77

  Fragments arranged by size: 

                 286     261      77
 
AvaI C'yCGr_G

Cuts at:      0     346     513     624 
   Size:        346     167     111

  Fragments arranged by size: 

                 346     167     111
 
BanI G'GyrC_C

Cuts at:      0      48     539     624 
   Size:         48     491      85

  Fragments arranged by size: 

                 491      85      48
 
BanII G_rGCy'C

Cuts at:      0     134     624 
   Size:        134     490
 
BglI GCCn_nnn'nGGC

Cuts at:      0     275     624 
   Size:        275     349
 
Bme1580I G_kGCm'C

Cuts at:      0      68     624 
   Size:         68     556
 
BsaAI yAC'GTr

Cuts at:      0     440     465     624 
   Size:        440      25     159

  Fragments arranged by size: 

                 440     159      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     624 
   Size:         58     566
 
BsmI GAATG_Cn'

Cuts at:      0     278     317     624 
   Size:        278      39     307

  Fragments arranged by size: 

                 307     278      39
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     134     624 
   Size:         68      66     490

  Fragments arranged by size: 

                 490      68      66
 
BsrFI r'CCGG_y

Cuts at:      0      75     624 
   Size:         75     549
 
BstBI TT'CG_AA

Cuts at:      0     419     624 
   Size:        419     205
 
Bsu36I CC'TnA_GG

Cuts at:      0       9     624 
   Size:          9     615
 
BtsI GCAGTG_nn'

Cuts at:      0     308     624 
   Size:        308     316
 
DraI TTT'AAA

Cuts at:      0     170     624 
   Size:        170     454
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     201     624 
   Size:        201     423
 
EcoO109I rG'GnC_Cy

Cuts at:      0     142     509     624 
   Size:        142     367     115

  Fragments arranged by size: 

                 367     142     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     134     166     329     361     624 
   Size:        134      32     163      32     263

  Fragments arranged by size: 

                 263     163     134      32      32
 
KpnI G_GTAC'C

Cuts at:      0      52     624 
   Size:         52     572
 
PasI CC'CwG_GG

Cuts at:      0     137     624 
   Size:        137     487
 
PmlI CAC'GTG

Cuts at:      0     465     624 
   Size:        465     159
 
PpuMI rG'GwC_Cy

Cuts at:      0     142     509     624 
   Size:        142     367     115

  Fragments arranged by size: 

                 367     142     115
 
PstI C_TGCA'G

Cuts at:      0     205     624 
   Size:        205     419
 
SanDI GG'GwC_CC

Cuts at:      0     142     509     624 
   Size:        142     367     115

  Fragments arranged by size: 

                 367     142     115
 
SfcI C'TryA_G

Cuts at:      0     201     624 
   Size:        201     423
 
SnaBI TAC'GTA

Cuts at:      0     440     624 
   Size:        440     184
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     271     624 
   Size:        271     353
 
XmnI GAAnn'nnTTC

Cuts at:      0      28     624 
   Size:         28     596
 

 Enzymes that do cut:

   Acc65I     AhdI     ApoI     AvaI     BanI    BanII     BglI Bme1580I
    BsaAI     BsgI     BsmI Bsp1286I    BsrFI    BstBI   Bsu36I     BtsI
     DraI     EciI EcoO109I    Hin4I     KpnI     PasI     PmlI    PpuMI
     PstI    SanDI     SfcI    SnaBI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI