Restriction map for
pFN21AE1700
(Linear) (Six-base) MAPSORT of: KIEE1700.seq Check: 6490 from: 1 to: 738
KIEE1700.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 100 388 738
Size: 82 18 288 350
Fragments arranged by size:
350 288 82 18
AflIII A'CryG_T
Cuts at: 0 215 738
Size: 215 523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 370 738
Size: 370 368
AlwNI CAG_nnn'CTG
Cuts at: 0 207 738
Size: 207 531
ApaI G_GGCC'C
Cuts at: 0 656 738
Size: 656 82
ApoI r'AATT_y
Cuts at: 0 552 738
Size: 552 186
AvaI C'yCGr_G
Cuts at: 0 62 738
Size: 62 676
BamHI G'GATC_C
Cuts at: 0 439 738
Size: 439 299
BanI G'GyrC_C
Cuts at: 0 275 738
Size: 275 463
BanII G_rGCy'C
Cuts at: 0 656 738
Size: 656 82
Bme1580I G_kGCm'C
Cuts at: 0 656 738
Size: 656 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 557 650 738
Size: 231 326 93 88
Fragments arranged by size:
326 231 93 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 416 738
Size: 336 80 322
Fragments arranged by size:
336 322 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 738
Size: 386 352
Bpu10I CC'TnA_GC
Cuts at: 0 626 738
Size: 626 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 738
Size: 101 637
BsaI GGTCTCn'nnnn_
Cuts at: 0 656 738
Size: 656 82
BsaAI yAC'GTr
Cuts at: 0 218 738
Size: 218 520
BsaWI w'CCGG_w
Cuts at: 0 158 738
Size: 158 580
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 738
Size: 309 429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 376 738
Size: 220 156 362
Fragments arranged by size:
362 220 156
BsiHKAI G_wGCw'C
Cuts at: 0 230 289 738
Size: 230 59 449
Fragments arranged by size:
449 230 59
Bsp1286I G_dGCh'C
Cuts at: 0 230 289 656 738
Size: 230 59 367 82
Fragments arranged by size:
367 230 82 59
BstYI r'GATC_y
Cuts at: 0 439 669 738
Size: 439 230 69
Fragments arranged by size:
439 230 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 516 738
Size: 516 222
EaeI y'GGCC_r
Cuts at: 0 267 738
Size: 267 471
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 180 738
Size: 180 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 100 386 388 738
Size: 82 18 286 2 350
Fragments arranged by size:
350 286 82 18 2
EcoO109I rG'GnC_Cy
Cuts at: 0 652 738
Size: 652 86
EcoRI G'AATT_C
Cuts at: 0 552 738
Size: 552 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 203 738
Size: 203 535
MfeI C'AATT_G
Cuts at: 0 456 738
Size: 456 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 738
Size: 65 673
MscI TGG'CCA
Cuts at: 0 269 738
Size: 269 469
NdeI CA'TA_TG
Cuts at: 0 481 738
Size: 481 257
NspI r_CATG'y
Cuts at: 0 470 738
Size: 470 268
PasI CC'CwG_GG
Cuts at: 0 240 738
Size: 240 498
PfoI T'CCnGG_A
Cuts at: 0 419 738
Size: 419 319
PspOMI G'GGCC_C
Cuts at: 0 652 738
Size: 652 86
SexAI A'CCwGG_T
Cuts at: 0 350 738
Size: 350 388
SmaI CCC'GGG
Cuts at: 0 64 738
Size: 64 674
SmlI C'TyrA_G
Cuts at: 0 116 738
Size: 116 622
SphI G_CATG'C
Cuts at: 0 470 738
Size: 470 268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 738
Size: 220 32 486
Fragments arranged by size:
486 220 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 544 738
Size: 544 194
XmaI C'CCGG_G
Cuts at: 0 62 738
Size: 62 676
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BseRI BsgI BsiHKAI Bsp1286I BstYI
DrdI EaeI EciI Eco57MI EcoO109I EcoRI FalI MfeI
MmeI MscI NdeI NspI PasI PfoI PspOMI SexAI
SmaI SmlI SphI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BsaBI BsaHI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII EagI EarI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII
TatI Tth111I XbaI XhoI XmnI ZraI