(Linear) (Six-base) MAPSORT of: KIEE1696.seq  Check: 2596  from: 1  to: 618



KIEE1696.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     399     618 
   Size:        399     219
 
AatII G_ACGT'C

Cuts at:      0     472     618 
   Size:        472     146
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     368     618 
   Size:         79     289     250

  Fragments arranged by size: 

                 289     250      79
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     317     349     487     519     618 
   Size:        317      32     138      32      99

  Fragments arranged by size: 

                 317     138      99      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     310     618 
   Size:        310     308
 
ApoI r'AATT_y

Cuts at:      0     316     359     618 
   Size:        316      43     259

  Fragments arranged by size: 

                 316     259      43
 
AvaI C'yCGr_G

Cuts at:      0     482     618 
   Size:        482     136
 
BanI G'GyrC_C

Cuts at:      0     411     618 
   Size:        411     207
 
BanII G_rGCy'C

Cuts at:      0      31     618 
   Size:         31     587
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     128     484     618 
   Size:        128     356     134

  Fragments arranged by size: 

                 356     134     128
 
BclI T'GATC_A

Cuts at:      0      50     618 
   Size:         50     568
 
BsaAI yAC'GTr

Cuts at:      0     462     618 
   Size:        462     156
 
BsaHI Gr'CG_yC

Cuts at:      0     469     618 
   Size:        469     149
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     317     347     618 
   Size:        317      30     271

  Fragments arranged by size: 

                 317     271      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     436     618 
   Size:        412      24     182

  Fragments arranged by size: 

                 412     182      24
 
BseYI C'CCAG_C

Cuts at:      0     208     618 
   Size:        208     410
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     618 
   Size:        475     143
 
BsiHKAI G_wGCw'C

Cuts at:      0      31     618 
   Size:         31     587
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     460     618 
   Size:        460     158
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     618 
   Size:         31     587
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     399     618 
   Size:        399     219
 
BsrBI CCG'CTC

Cuts at:      0     402     618 
   Size:        402     216
 
BtsI GCAGTG_nn'

Cuts at:      0     364     618 
   Size:        364     254
 
DraI TTT'AAA

Cuts at:      0     597     618 
   Size:        597      21
 
DraIII CAC_nnn'GTG

Cuts at:      0      48     618 
   Size:         48     570
 
EarI CTCTTCn'nnn_

Cuts at:      0     437     618 
   Size:        437     181
 
EcoICRI GAG'CTC

Cuts at:      0      29     618 
   Size:         29     589
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     368     618 
   Size:         79     289     250

  Fragments arranged by size: 

                 289     250      79
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      43     618 
   Size:         43     575
 
EcoRI G'AATT_C

Cuts at:      0     316     618 
   Size:        316     302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     184     283     522     618 
   Size:        184      99     239      96

  Fragments arranged by size: 

                 239     184      99      96
 
MspA1I CmG'CkG

Cuts at:      0      65     208     618 
   Size:         65     143     410

  Fragments arranged by size: 

                 410     143      65
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     317     349     618 
   Size:        317      32     269

  Fragments arranged by size: 

                 317     269      32
 
SacI G_AGCT'C

Cuts at:      0      31     618 
   Size:         31     587
 
SmaI CCC'GGG

Cuts at:      0     484     618 
   Size:        484     134
 
StyI C'CwwG_G

Cuts at:      0      32     618 
   Size:         32     586
 
XmaI C'CCGG_G

Cuts at:      0     482     618 
   Size:        482     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     182     263     329     618 
   Size:        182      81      66     289

  Fragments arranged by size: 

                 289     182      81      66
 
ZraI GAC'GTC

Cuts at:      0     470     618 
   Size:        470     148
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AloI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI     BclI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI     BtsI     DraI
   DraIII     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI   MspA1I
     PpiI     SacI     SmaI     StyI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DrdI     EaeI     EagI     EciI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI